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Strain Name:
C57BL/6J-MtgxR6158Btlr/Mmmh
Stock Number:
044305-MU
Citation ID:
RRID:MMRRC_044305-MU
Other Names:
R6158 (G1)
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Kl
Name: klotho
Synonyms: alpha-kl
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16591
HGNC: HGNC:6344
Homologene: 68415
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Mix23
Name: mitochondrial matrix import factor 23
Synonyms: A930007B11Rik, Ccdc58
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 381045
VEGA: 16
Homologene: 18028
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 79,435,400 bp
  • A to T, chromosome 1 at 84,761,012 bp
  • T to C, chromosome 1 at 153,804,021 bp
  • T to C, chromosome 1 at 162,320,987 bp
  • A to G, chromosome 2 at 14,985,601 bp
  • G to T, chromosome 2 at 23,132,772 bp
  • A to T, chromosome 2 at 36,569,116 bp
  • A to G, chromosome 2 at 86,851,515 bp
  • T to C, chromosome 2 at 88,831,146 bp
  • A to T, chromosome 2 at 110,665,875 bp
  • T to C, chromosome 2 at 120,301,071 bp
  • A to T, chromosome 2 at 120,360,103 bp
  • T to C, chromosome 2 at 129,130,517 bp
  • A to T, chromosome 2 at 146,424,676 bp
  • C to A, chromosome 2 at 152,873,104 bp
  • G to T, chromosome 2 at 167,056,726 bp
  • A to C, chromosome 2 at 172,363,596 bp
  • A to T, chromosome 2 at 174,643,858 bp
  • A to T, chromosome 3 at 38,983,262 bp
  • A to G, chromosome 4 at 34,029,176 bp
  • A to T, chromosome 4 at 45,285,401 bp
  • A to G, chromosome 4 at 138,967,254 bp
  • T to C, chromosome 5 at 34,907,086 bp
  • A to T, chromosome 5 at 102,892,912 bp
  • A to T, chromosome 5 at 112,874,172 bp
  • A to C, chromosome 5 at 117,867,574 bp
  • T to A, chromosome 5 at 128,747,876 bp
  • C to T, chromosome 5 at 134,940,576 bp
  • T to C, chromosome 5 at 150,454,572 bp
  • T to A, chromosome 5 at 150,988,853 bp
  • A to T, chromosome 6 at 3,994,311 bp
  • T to A, chromosome 6 at 40,757,714 bp
  • T to A, chromosome 6 at 57,036,289 bp
  • A to G, chromosome 7 at 4,125,562 bp
  • A to G, chromosome 7 at 12,512,672 bp
  • T to A, chromosome 7 at 42,933,680 bp
  • T to A, chromosome 7 at 89,156,444 bp
  • T to A, chromosome 7 at 110,270,023 bp
  • C to T, chromosome 8 at 13,224,977 bp
  • G to T, chromosome 8 at 33,319,172 bp
  • C to T, chromosome 9 at 39,737,076 bp
  • A to T, chromosome 9 at 44,108,970 bp
  • G to T, chromosome 9 at 81,927,576 bp
  • G to T, chromosome 9 at 108,964,603 bp
  • A to T, chromosome 9 at 110,087,030 bp
  • C to T, chromosome 10 at 12,690,822 bp
  • T to A, chromosome 10 at 24,284,777 bp
  • C to T, chromosome 11 at 60,232,927 bp
  • T to G, chromosome 11 at 62,118,124 bp
  • T to A, chromosome 11 at 99,914,918 bp
  • A to G, chromosome 12 at 16,954,918 bp
  • T to C, chromosome 12 at 71,170,636 bp
  • T to A, chromosome 13 at 38,210,236 bp
  • T to C, chromosome 13 at 55,245,621 bp
  • A to T, chromosome 14 at 26,773,685 bp
  • G to A, chromosome 14 at 51,894,224 bp
  • T to A, chromosome 14 at 101,900,137 bp
  • A to T, chromosome 15 at 81,399,601 bp
  • A to C, chromosome 15 at 90,571,988 bp
  • C to A, chromosome 16 at 19,605,925 bp
  • T to A, chromosome 16 at 36,089,929 bp
  • A to G, chromosome 17 at 5,986,212 bp
  • T to A, chromosome 17 at 32,978,389 bp
  • T to G, chromosome 17 at 58,973,627 bp
  • T to C, chromosome 18 at 31,988,549 bp
  • C to T, chromosome 19 at 10,644,221 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6158 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044305-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.