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Strain Name:
C57BL/6J-MtgxR6167Btlr/Mmmh
Stock Number:
044313-MU
Citation ID:
RRID:MMRRC_044313-MU
Other Names:
R6167 (G1)
Major Collection:

Strain Information

Camkk2
Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
Synonyms: 6330570N16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 207565
HGNC: HGNC:1470
Homologene: 32756
Atn1
Name: atrophin 1
Synonyms: atrophin-1, Atr1, Drpla
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13498
HGNC: HGNC:3033
Homologene: 1461
Etv1
Name: ets variant 1
Synonyms: ER81, Etsrp81
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14009
HGNC: HGNC:3490
Homologene: 3636
Slc1a6
Name: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
Synonyms: EAAT4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20513
VEGA: 10
Homologene: 21055
Iars1
Name: isoleucyl-tRNA synthetase 1
Synonyms: E430001P04Rik, 2510016L12Rik, Iars
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105148
HGNC: HGNC:5330
Homologene: 5325
Sh3glb1
Name: SH3-domain GRB2-like B1 (endophilin)
Synonyms: Bif-1, Endophilin B1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54673
Homologene: 9337
Gtf2f1
Name: general transcription factor IIF, polypeptide 1
Synonyms: 2810405L04Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 98053
VEGA: 17
HGNC: HGNC:4652
Homologene: 1585
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 23,376,228 bp
  • G to A, chromosome 1 at 34,819,603 bp
  • C to T, chromosome 1 at 58,263,935 bp
  • A to G, chromosome 1 at 133,279,407 bp
  • T to A, chromosome 2 at 52,147,237 bp
  • A to C, chromosome 2 at 74,659,262 bp
  • G to A, chromosome 2 at 92,367,088 bp
  • A to G, chromosome 2 at 152,915,753 bp
  • C to T, chromosome 3 at 129,988,901 bp
  • A to T, chromosome 3 at 144,691,903 bp
  • A to G, chromosome 4 at 104,945,464 bp
  • A to T, chromosome 5 at 112,872,507 bp
  • A to T, chromosome 5 at 121,828,355 bp
  • A to G, chromosome 5 at 122,764,124 bp
  • T to C, chromosome 6 at 124,746,737 bp
  • G to A, chromosome 7 at 19,861,257 bp
  • T to C, chromosome 8 at 23,245,888 bp
  • T to C, chromosome 8 at 48,254,622 bp
  • T to C, chromosome 8 at 84,097,997 bp
  • T to C, chromosome 8 at 84,995,013 bp
  • G to A, chromosome 8 at 95,559,545 bp
  • A to G, chromosome 8 at 105,270,849 bp
  • T to A, chromosome 9 at 106,156,492 bp
  • A to T, chromosome 9 at 119,066,864 bp
  • A to G, chromosome 10 at 78,801,837 bp
  • C to T, chromosome 10 at 91,162,938 bp
  • T to C, chromosome 10 at 119,897,797 bp
  • C to T, chromosome 10 at 127,517,862 bp
  • G to A, chromosome 11 at 4,915,497 bp
  • A to G, chromosome 11 at 73,665,334 bp
  • C to A, chromosome 11 at 100,411,845 bp
  • A to T, chromosome 11 at 106,037,251 bp
  • T to A, chromosome 11 at 110,292,105 bp
  • A to G, chromosome 11 at 111,072,489 bp
  • A to G, chromosome 11 at 115,615,551 bp
  • G to A, chromosome 12 at 29,974,001 bp
  • A to T, chromosome 12 at 38,865,641 bp
  • A to T, chromosome 12 at 65,094,895 bp
  • T to C, chromosome 12 at 112,783,122 bp
  • T to A, chromosome 13 at 34,344,559 bp
  • C to A, chromosome 13 at 41,053,352 bp
  • T to A, chromosome 13 at 49,722,714 bp
  • C to A, chromosome 14 at 50,534,155 bp
  • A to C, chromosome 14 at 70,422,893 bp
  • C to A, chromosome 15 at 97,767,411 bp
  • C to T, chromosome 16 at 55,233,599 bp
  • T to C, chromosome 16 at 63,612,924 bp
  • T to G, chromosome 17 at 21,587,649 bp
  • T to C, chromosome 17 at 37,063,379 bp
  • G to T, chromosome 17 at 57,004,161 bp
  • A to G, chromosome 18 at 34,428,244 bp
  • A to T, chromosome 18 at 43,313,409 bp
  • T to G, chromosome 19 at 4,115,188 bp
  • T to C, chromosome 19 at 4,273,053 bp
  • T to C, chromosome 19 at 46,515,187 bp
  • G to C, chromosome 19 at 47,239,928 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6167 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044313-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.