Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6181Btlr/Mmmh
Stock Number:
044323-MU
Citation ID:
RRID:MMRRC_044323-MU
Other Names:
R6181 (G1)
Major Collection:

Strain Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Rbm47
Name: RNA binding motif protein 47
Synonyms: 9530077J19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 245945
Homologene: 36932
Lrp5
Name: low density lipoprotein receptor-related protein 5
Synonyms: LRP7, LR3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16973
HGNC: HGNC:6697
Homologene: 1746
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 14,302,872 bp
  • C to T, chromosome 1 at 54,512,777 bp
  • T to C, chromosome 1 at 58,158,946 bp
  • T to A, chromosome 1 at 90,816,374 bp
  • T to C, chromosome 1 at 93,395,359 bp
  • T to G, chromosome 1 at 118,419,817 bp
  • T to A, chromosome 1 at 133,132,523 bp
  • T to C, chromosome 1 at 150,333,270 bp
  • G to A, chromosome 2 at 13,349,876 bp
  • A to G, chromosome 2 at 36,251,119 bp
  • G to T, chromosome 2 at 82,257,142 bp
  • A to G, chromosome 3 at 59,209,907 bp
  • T to A, chromosome 3 at 88,635,620 bp
  • A to T, chromosome 3 at 95,127,807 bp
  • G to A, chromosome 3 at 127,587,941 bp
  • C to A, chromosome 3 at 133,487,759 bp
  • A to T, chromosome 3 at 145,090,708 bp
  • A to G, chromosome 4 at 32,715,953 bp
  • G to C, chromosome 4 at 96,536,086 bp
  • A to G, chromosome 4 at 136,543,267 bp
  • T to A, chromosome 4 at 143,616,258 bp
  • T to C, chromosome 4 at 152,308,848 bp
  • A to G, chromosome 5 at 31,488,055 bp
  • T to A, chromosome 5 at 34,443,540 bp
  • T to C, chromosome 5 at 66,026,490 bp
  • A to G, chromosome 5 at 69,561,716 bp
  • A to G, chromosome 5 at 72,372,096 bp
  • T to A, chromosome 5 at 139,716,751 bp
  • T to C, chromosome 5 at 146,483,772 bp
  • C to T, chromosome 5 at 151,647,520 bp
  • A to G, chromosome 6 at 4,619,331 bp
  • T to C, chromosome 6 at 17,694,364 bp
  • A to T, chromosome 6 at 30,505,971 bp
  • A to G, chromosome 6 at 34,415,378 bp
  • T to C, chromosome 6 at 39,485,620 bp
  • C to T, chromosome 6 at 46,759,808 bp
  • T to A, chromosome 6 at 70,340,984 bp
  • T to A, chromosome 6 at 118,548,877 bp
  • G to T, chromosome 6 at 125,566,146 bp
  • A to T, chromosome 7 at 3,683,885 bp
  • C to A, chromosome 7 at 23,606,267 bp
  • T to A, chromosome 7 at 24,003,215 bp
  • A to G, chromosome 7 at 128,297,460 bp
  • T to A, chromosome 7 at 135,158,522 bp
  • A to C, chromosome 8 at 104,356,365 bp
  • G to A, chromosome 9 at 40,207,342 bp
  • A to T, chromosome 9 at 106,501,975 bp
  • A to G, chromosome 10 at 12,739,456 bp
  • T to C, chromosome 10 at 72,656,599 bp
  • A to C, chromosome 10 at 127,050,410 bp
  • T to A, chromosome 11 at 32,742,575 bp
  • T to A, chromosome 11 at 49,552,293 bp
  • A to G, chromosome 11 at 97,242,004 bp
  • T to C, chromosome 11 at 104,831,333 bp
  • G to A, chromosome 12 at 30,131,885 bp
  • A to G, chromosome 12 at 98,699,999 bp
  • T to A, chromosome 13 at 22,187,282 bp
  • T to C, chromosome 13 at 65,112,584 bp
  • A to G, chromosome 13 at 75,910,689 bp
  • C to T, chromosome 13 at 95,086,808 bp
  • T to A, chromosome 14 at 27,472,278 bp
  • T to C, chromosome 15 at 78,539,461 bp
  • T to A, chromosome 16 at 3,933,744 bp
  • A to G, chromosome 16 at 45,417,897 bp
  • T to C, chromosome 16 at 90,779,094 bp
  • T to C, chromosome 17 at 18,073,757 bp
  • G to A, chromosome 17 at 18,583,864 bp
  • T to C, chromosome 17 at 23,953,492 bp
  • A to G, chromosome 17 at 25,151,728 bp
  • A to G, chromosome 17 at 28,810,581 bp
  • G to A, chromosome 17 at 30,748,568 bp
  • A to G, chromosome 17 at 43,628,897 bp
  • A to G, chromosome 18 at 54,895,628 bp
  • T to C, chromosome 19 at 3,628,427 bp
  • C to T, chromosome 19 at 8,823,232 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6181 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044323-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.