Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6188Btlr/Mmmh
Stock Number:
044328-MU
Citation ID:
RRID:MMRRC_044328-MU
Other Names:
R6188 (G1)
Major Collection:

Strain Information

a
Name: nonagouti
Synonyms: agouti, As, agouti signal protein, ASP, Asip
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Epb41l4a
Name: erythrocyte membrane protein band 4.1 like 4a
Synonyms: NBL4, Epb4.1l4a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13824
VEGA: 18
Homologene: 8398
Exoc6b
Name: exocyst complex component 6B
Synonyms: 4930569O18Rik, G430127E12Rik, Sec15b, Sec15l2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75914
Homologene: 44781
Nup155
Name: nucleoporin 155
Synonyms: D930027M19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170762
VEGA: 15
HGNC: HGNC:8063
Homologene: 43155
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Rlf
Name: rearranged L-myc fusion sequence
Synonyms: 9230110M18Rik, MommeD8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109263
Homologene: 8243
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, Aspm, D330028K02Rik, MCPH5, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • GGAGAGAG to GGAGAG, chromosome 1 at 5,020,883 bp
  • C to A, chromosome 1 at 24,711,545 bp
  • T to A, chromosome 1 at 26,685,703 bp
  • A to G, chromosome 1 at 107,078,507 bp
  • C to A, chromosome 1 at 139,479,239 bp
  • A to G, chromosome 2 at 4,927,490 bp
  • T to C, chromosome 2 at 25,609,752 bp
  • C to A, chromosome 2 at 28,079,190 bp
  • A to T, chromosome 2 at 89,575,194 bp
  • A to G, chromosome 2 at 119,139,350 bp
  • A to G, chromosome 2 at 122,319,794 bp
  • A to T, chromosome 2 at 155,047,682 bp
  • G to A, chromosome 3 at 30,937,049 bp
  • A to G, chromosome 3 at 88,098,893 bp
  • A to G, chromosome 3 at 94,402,850 bp
  • T to C, chromosome 3 at 133,480,326 bp
  • A to T, chromosome 4 at 3,185,017 bp
  • C to T, chromosome 4 at 88,785,859 bp
  • A to T, chromosome 4 at 106,745,499 bp
  • T to C, chromosome 4 at 121,170,766 bp
  • C to T, chromosome 4 at 155,610,026 bp
  • A to C, chromosome 5 at 53,155,719 bp
  • G to T, chromosome 5 at 144,992,844 bp
  • A to T, chromosome 6 at 5,158,113 bp
  • A to G, chromosome 6 at 84,855,497 bp
  • A to G, chromosome 6 at 91,763,533 bp
  • C to A, chromosome 7 at 19,698,380 bp
  • A to T, chromosome 7 at 131,097,631 bp
  • A to G, chromosome 8 at 22,647,447 bp
  • C to T, chromosome 8 at 112,008,481 bp
  • A to G, chromosome 9 at 22,249,990 bp
  • T to C, chromosome 10 at 77,582,508 bp
  • A to G, chromosome 10 at 82,285,257 bp
  • T to A, chromosome 11 at 34,503,396 bp
  • T to C, chromosome 11 at 58,446,356 bp
  • A to G, chromosome 11 at 82,103,117 bp
  • C to T, chromosome 11 at 94,991,836 bp
  • A to T, chromosome 11 at 100,102,420 bp
  • A to T, chromosome 13 at 23,183,914 bp
  • T to C, chromosome 13 at 37,025,778 bp
  • T to A, chromosome 13 at 53,111,311 bp
  • A to G, chromosome 13 at 58,394,153 bp
  • A to T, chromosome 13 at 77,183,731 bp
  • A to G, chromosome 13 at 93,093,444 bp
  • A to G, chromosome 13 at 93,097,276 bp
  • T to A, chromosome 14 at 28,317,251 bp
  • C to T, chromosome 14 at 53,559,957 bp
  • C to T, chromosome 14 at 72,589,961 bp
  • T to C, chromosome 15 at 8,109,575 bp
  • T to C, chromosome 15 at 82,059,270 bp
  • A to G, chromosome 15 at 96,692,516 bp
  • A to G, chromosome 16 at 19,286,557 bp
  • T to C, chromosome 16 at 21,984,268 bp
  • G to A, chromosome 16 at 44,499,548 bp
  • A to T, chromosome 16 at 66,815,307 bp
  • T to C, chromosome 16 at 88,056,071 bp
  • A to G, chromosome 17 at 21,659,019 bp
  • G to T, chromosome 17 at 25,241,861 bp
  • T to A, chromosome 18 at 33,832,665 bp
  • T to C, chromosome 18 at 37,708,271 bp
  • T to C, chromosome 18 at 53,724,457 bp
  • G to A, chromosome 19 at 6,397,937 bp
  • A to G, chromosome 19 at 12,422,610 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6188 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044328-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.