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Strain Name:
C57BL/6J-MtgxR6190Btlr/Mmmh
Stock Number:
044330-MU
Citation ID:
RRID:MMRRC_044330-MU
Other Names:
R6190 (G1)
Major Collection:

Strain Information

Pax3
Name: paired box 3
Synonyms: Pax-3, Splchl2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Htr1d
Name: 5-hydroxytryptamine (serotonin) receptor 1D
Synonyms: Gpcr14, Htr1db
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 15552
HGNC: HGNC:5289
Homologene: 20240
Becn1
Name: beclin 1, autophagy related
Synonyms: Atg6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56208
HGNC: HGNC:1034
Homologene: 2794
Zmym1
Name: zinc finger, MYM domain containing 1
Synonyms: 5830412B09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68310
Homologene: 32904
Llgl2
Name: LLGL2 scribble cell polarity complex component
Synonyms: 9130006H11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217325
HGNC: HGNC:6629
Homologene: 3323
Ak2
Name: adenylate kinase 2
Synonyms: Ak-2, D4Ertd220e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11637
HGNC: HGNC:362
Homologene: 1227
Gria4
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)
Synonyms: Glur-4, Glur4, spkw1, Gluralpha4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14802
HGNC: HGNC:4574
Homologene: 20227
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 25,420,647 bp
  • C to T, chromosome 1 at 78,192,549 bp
  • T to A, chromosome 1 at 100,379,075 bp
  • T to C, chromosome 1 at 139,133,675 bp
  • C to A, chromosome 1 at 154,486,570 bp
  • G to A, chromosome 1 at 166,630,323 bp
  • T to A, chromosome 1 at 176,782,409 bp
  • T to C, chromosome 2 at 10,098,507 bp
  • C to T, chromosome 2 at 83,876,698 bp
  • T to A, chromosome 2 at 86,228,234 bp
  • C to T, chromosome 2 at 145,947,133 bp
  • A to G, chromosome 3 at 55,487,811 bp
  • A to G, chromosome 3 at 64,538,003 bp
  • T to A, chromosome 3 at 88,023,293 bp
  • T to C, chromosome 3 at 89,091,881 bp
  • A to G, chromosome 3 at 94,363,919 bp
  • A to C, chromosome 3 at 122,729,307 bp
  • A to G, chromosome 3 at 144,758,060 bp
  • A to G, chromosome 4 at 101,133,617 bp
  • A to T, chromosome 4 at 101,175,128 bp
  • A to T, chromosome 4 at 108,367,855 bp
  • C to T, chromosome 4 at 109,861,815 bp
  • T to C, chromosome 4 at 127,047,884 bp
  • A to G, chromosome 4 at 128,999,183 bp
  • A to G, chromosome 4 at 132,775,020 bp
  • T to C, chromosome 4 at 136,442,798 bp
  • G to A, chromosome 4 at 140,542,762 bp
  • T to A, chromosome 4 at 152,041,234 bp
  • A to G, chromosome 5 at 5,675,881 bp
  • A to G, chromosome 5 at 25,831,690 bp
  • T to C, chromosome 5 at 36,571,884 bp
  • A to T, chromosome 5 at 93,638,078 bp
  • A to G, chromosome 5 at 110,102,603 bp
  • T to C, chromosome 5 at 122,551,100 bp
  • T to C, chromosome 6 at 48,678,351 bp
  • T to A, chromosome 6 at 83,003,896 bp
  • T to A, chromosome 6 at 89,356,604 bp
  • T to C, chromosome 6 at 120,475,273 bp
  • T to A, chromosome 6 at 132,312,729 bp
  • A to T, chromosome 7 at 12,494,001 bp
  • G to T, chromosome 7 at 18,655,001 bp
  • G to A, chromosome 7 at 19,201,163 bp
  • A to G, chromosome 7 at 30,425,737 bp
  • A to T, chromosome 7 at 45,102,198 bp
  • A to T, chromosome 7 at 64,739,880 bp
  • T to C, chromosome 7 at 107,991,100 bp
  • A to G, chromosome 7 at 114,523,032 bp
  • T to C, chromosome 8 at 75,023,299 bp
  • C to T, chromosome 8 at 88,325,730 bp
  • T to A, chromosome 9 at 4,420,199 bp
  • T to A, chromosome 9 at 56,619,650 bp
  • T to G, chromosome 9 at 66,376,381 bp
  • A to G, chromosome 9 at 110,374,067 bp
  • G to T, chromosome 9 at 114,632,723 bp
  • T to A, chromosome 9 at 121,664,653 bp
  • C to A, chromosome 10 at 4,356,268 bp
  • A to G, chromosome 10 at 18,470,041 bp
  • A to G, chromosome 10 at 26,846,035 bp
  • C to G, chromosome 10 at 41,422,407 bp
  • A to T, chromosome 10 at 41,422,408 bp
  • T to C, chromosome 10 at 91,164,207 bp
  • T to C, chromosome 10 at 130,425,461 bp
  • T to C, chromosome 11 at 88,076,186 bp
  • T to C, chromosome 11 at 94,548,236 bp
  • T to A, chromosome 11 at 95,574,441 bp
  • C to T, chromosome 11 at 99,485,758 bp
  • A to G, chromosome 11 at 101,295,374 bp
  • G to T, chromosome 11 at 103,501,216 bp
  • G to A, chromosome 11 at 115,846,986 bp
  • A to C, chromosome 12 at 31,344,848 bp
  • A to G, chromosome 12 at 71,126,350 bp
  • T to C, chromosome 12 at 86,736,489 bp
  • C to T, chromosome 13 at 52,611,053 bp
  • T to A, chromosome 13 at 81,459,763 bp
  • A to T, chromosome 13 at 81,524,779 bp
  • C to A, chromosome 13 at 85,233,695 bp
  • T to A, chromosome 14 at 24,190,438 bp
  • C to T, chromosome 14 at 29,991,717 bp
  • C to A, chromosome 14 at 31,363,603 bp
  • A to G, chromosome 14 at 120,385,421 bp
  • T to C, chromosome 15 at 20,665,785 bp
  • C to T, chromosome 15 at 54,571,398 bp
  • C to A, chromosome 15 at 81,438,099 bp
  • T to A, chromosome 16 at 8,605,608 bp
  • T to C, chromosome 16 at 17,632,013 bp
  • T to C, chromosome 16 at 18,284,410 bp
  • A to G, chromosome 16 at 20,392,779 bp
  • T to A, chromosome 16 at 31,449,897 bp
  • A to T, chromosome 16 at 75,870,915 bp
  • G to A, chromosome 17 at 21,314,294 bp
  • A to G, chromosome 17 at 21,397,268 bp
  • A to G, chromosome 17 at 28,071,717 bp
  • A to T, chromosome 17 at 32,929,873 bp
  • A to G, chromosome 17 at 37,262,744 bp
  • G to A, chromosome 17 at 45,408,017 bp
  • C to A, chromosome 18 at 24,004,061 bp
  • T to G, chromosome 18 at 36,611,809 bp
  • A to G, chromosome 18 at 80,712,670 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6190 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044330-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.