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Strain Name:
C57BL/6J-MtgxR6224Btlr/Mmmh
Stock Number:
044355-MU
Citation ID:
RRID:MMRRC_044355-MU
Other Names:
R6224 (G1)
Major Collection:

Strain Information

Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Zc3h13
Name: zinc finger CCCH type containing 13
Synonyms: C87618, 4930570G11Rik, 2600010B19Rik, 3110050K21Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67302
VEGA: 14
Dcun1d3
Name: defective in cullin neddylation 1 domain containing 3
Synonyms: 1700020A13Rik, DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae)
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233805
Homologene: 16155
Gspt1
Name: G1 to S phase transition 1
Synonyms: Gst-1, Gst-1, G1st
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 14852
HGNC: HGNC:4621
Homologene: 68226
Brd1
Name: bromodomain containing 1
Synonyms: 1110059H06Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223770
HGNC: HGNC:1102
Homologene: 40956
Skic3
Name: SKI3 subunit of superkiller complex
Synonyms: Ttc37
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218343
VEGA: 13
Homologene: 40966
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 24,401,557 bp
  • C to T, chromosome 2 at 25,610,582 bp
  • G to T, chromosome 2 at 91,054,630 bp
  • T to A, chromosome 2 at 132,849,850 bp
  • G to A, chromosome 2 at 153,987,153 bp
  • T to A, chromosome 3 at 52,345,672 bp
  • C to A, chromosome 3 at 59,325,510 bp
  • C to G, chromosome 3 at 93,398,130 bp
  • T to C, chromosome 3 at 95,681,729 bp
  • T to C, chromosome 3 at 135,243,775 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • A to G, chromosome 4 at 132,502,570 bp
  • T to C, chromosome 5 at 137,812,036 bp
  • A to T, chromosome 6 at 72,616,001 bp
  • A to T, chromosome 7 at 4,394,212 bp
  • A to G, chromosome 7 at 27,878,582 bp
  • G to A, chromosome 7 at 45,261,148 bp
  • T to C, chromosome 7 at 108,066,742 bp
  • A to T, chromosome 7 at 119,859,491 bp
  • T to C, chromosome 7 at 132,143,731 bp
  • A to T, chromosome 8 at 85,067,591 bp
  • T to A, chromosome 8 at 123,305,281 bp
  • T to A, chromosome 9 at 35,136,481 bp
  • T to A, chromosome 10 at 51,491,649 bp
  • A to G, chromosome 10 at 103,215,757 bp
  • A to G, chromosome 10 at 129,857,192 bp
  • A to C, chromosome 11 at 30,454,815 bp
  • T to C, chromosome 11 at 48,866,886 bp
  • T to C, chromosome 11 at 70,098,246 bp
  • T to A, chromosome 11 at 72,855,383 bp
  • A to C, chromosome 11 at 99,838,014 bp
  • T to C, chromosome 12 at 44,283,028 bp
  • T to C, chromosome 12 at 108,361,560 bp
  • T to C, chromosome 12 at 108,514,508 bp
  • T to C, chromosome 13 at 6,565,054 bp
  • T to C, chromosome 13 at 15,725,145 bp
  • T to C, chromosome 13 at 55,313,132 bp
  • T to A, chromosome 13 at 59,706,320 bp
  • C to T, chromosome 13 at 76,118,291 bp
  • T to A, chromosome 14 at 31,050,111 bp
  • G to A, chromosome 14 at 32,342,061 bp
  • T to C, chromosome 14 at 56,668,353 bp
  • T to A, chromosome 14 at 75,337,409 bp
  • A to G, chromosome 14 at 108,912,022 bp
  • GACTAC to GACTACTAC, chromosome 14 at 111,679,816 bp
  • A to T, chromosome 15 at 88,688,355 bp
  • A to T, chromosome 15 at 101,850,267 bp
  • C to A, chromosome 16 at 11,224,542 bp
  • A to T, chromosome 16 at 66,664,395 bp
  • T to A, chromosome 16 at 79,024,378 bp
  • A to G, chromosome 17 at 46,640,062 bp
  • A to G, chromosome 17 at 49,096,351 bp
  • T to C, chromosome 17 at 67,802,987 bp
  • C to A, chromosome 18 at 61,081,939 bp
  • T to A, chromosome 19 at 6,227,112 bp
  • T to C, chromosome 19 at 32,698,628 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6224 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044355-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.