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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR6228Btlr/Mmmh
Stock Number:
044357-MU
Citation ID:
RRID:MMRRC_044357-MU
Other Names:
R6228 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Fam117b
Name: family with sequence similarity 117, member B
Synonyms: 6330416D14Rik, 2810425F24Rik, Als2cr13
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72750
Homologene: 18307

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Zdhhc17
Name: zinc finger, DHHC domain containing 17
Synonyms: A230053P19Rik, D130071N24Rik, Hip14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320150
VEGA: 10
Homologene: 56324

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Gene Ontology
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ClinGen
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953

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ClinGen
Tlx3
Name: T cell leukemia, homeobox 3
Synonyms: Tlx1l2, Rnx, Hox11l2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 27140
Homologene: 23181

Additional Resources
IMPC
Google
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PubMed
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Gene Ontology
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Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Ep400
Name: E1A binding protein p400
Synonyms: p400, mDomino, 1700020J09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75560
Homologene: 38779

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Dgat1
Name: diacylglycerol O-acyltransferase 1
Synonyms: D15Ertd23e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 13350
HGNC: HGNC:2843
Homologene: 7688

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 33,803,138 bp
  • G to T, chromosome 1 at 59,867,436 bp
  • A to G, chromosome 1 at 59,969,048 bp
  • C to A, chromosome 1 at 60,295,924 bp
  • A to C, chromosome 1 at 128,592,183 bp
  • T to C, chromosome 1 at 161,970,467 bp
  • G to A, chromosome 1 at 174,152,146 bp
  • G to A, chromosome 1 at 183,099,025 bp
  • G to A, chromosome 2 at 14,271,304 bp
  • A to G, chromosome 2 at 30,256,553 bp
  • T to G, chromosome 2 at 69,482,366 bp
  • A to T, chromosome 2 at 76,810,446 bp
  • T to C, chromosome 2 at 86,978,691 bp
  • A to G, chromosome 2 at 120,713,750 bp
  • A to T, chromosome 2 at 122,287,193 bp
  • C to T, chromosome 2 at 148,698,178 bp
  • T to C, chromosome 2 at 170,119,577 bp
  • G to T, chromosome 3 at 79,612,029 bp
  • A to T, chromosome 3 at 100,022,602 bp
  • A to G, chromosome 3 at 104,792,981 bp
  • G to C, chromosome 3 at 107,448,377 bp
  • A to T, chromosome 3 at 116,351,213 bp
  • G to A, chromosome 3 at 123,671,652 bp
  • A to T, chromosome 3 at 154,239,163 bp
  • T to C, chromosome 4 at 43,304,674 bp
  • C to T, chromosome 4 at 62,402,794 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • A to T, chromosome 5 at 67,016,502 bp
  • A to G, chromosome 5 at 101,898,429 bp
  • A to G, chromosome 5 at 110,670,942 bp
  • T to C, chromosome 6 at 70,336,097 bp
  • C to A, chromosome 6 at 71,823,677 bp
  • C to T, chromosome 7 at 42,042,411 bp
  • T to C, chromosome 7 at 45,083,296 bp
  • T to A, chromosome 7 at 45,819,497 bp
  • G to T, chromosome 7 at 46,030,256 bp
  • T to C, chromosome 7 at 107,074,136 bp
  • T to C, chromosome 7 at 139,656,580 bp
  • A to G, chromosome 8 at 69,816,123 bp
  • TCGACG to TCG, chromosome 8 at 71,890,708 bp
  • A to G, chromosome 9 at 50,607,671 bp
  • A to C, chromosome 9 at 50,703,356 bp
  • A to G, chromosome 9 at 75,006,363 bp
  • T to C, chromosome 9 at 101,923,584 bp
  • A to G, chromosome 9 at 107,260,063 bp
  • A to G, chromosome 10 at 58,235,522 bp
  • A to T, chromosome 10 at 91,122,228 bp
  • T to C, chromosome 10 at 110,956,355 bp
  • T to C, chromosome 10 at 115,372,846 bp
  • C to A, chromosome 11 at 33,202,432 bp
  • T to C, chromosome 11 at 55,124,951 bp
  • A to T, chromosome 11 at 58,386,803 bp
  • A to T, chromosome 11 at 67,087,486 bp
  • A to G, chromosome 12 at 83,065,964 bp
  • G to A, chromosome 12 at 102,484,481 bp
  • A to G, chromosome 12 at 115,765,353 bp
  • A to G, chromosome 12 at 117,721,663 bp
  • T to A, chromosome 13 at 14,346,038 bp
  • C to A, chromosome 13 at 23,076,463 bp
  • A to T, chromosome 13 at 23,464,351 bp
  • C to A, chromosome 13 at 47,027,259 bp
  • A to G, chromosome 13 at 73,821,460 bp
  • T to C, chromosome 13 at 92,708,108 bp
  • T to C, chromosome 14 at 30,931,260 bp
  • T to C, chromosome 14 at 31,707,220 bp
  • A to T, chromosome 14 at 34,056,599 bp
  • T to C, chromosome 14 at 54,626,189 bp
  • T to A, chromosome 14 at 103,260,229 bp
  • G to T, chromosome 15 at 9,310,640 bp
  • T to C, chromosome 15 at 76,503,293 bp
  • T to G, chromosome 15 at 79,305,724 bp
  • T to C, chromosome 15 at 83,967,624 bp
  • A to T, chromosome 15 at 99,723,265 bp
  • C to T, chromosome 16 at 57,182,903 bp
  • C to A, chromosome 17 at 24,442,518 bp
  • C to G, chromosome 17 at 24,507,180 bp
  • T to C, chromosome 17 at 32,775,884 bp
  • G to T, chromosome 17 at 34,093,877 bp
  • C to T, chromosome 17 at 55,999,976 bp
  • T to C, chromosome 18 at 20,594,293 bp
  • A to T, chromosome 18 at 32,842,006 bp
  • A to T, chromosome 18 at 34,695,793 bp
  • A to G, chromosome 18 at 37,356,984 bp
  • A to G, chromosome 18 at 37,402,062 bp
  • G to T, chromosome 18 at 77,948,462 bp
  • A to C, chromosome 18 at 78,116,399 bp
  • T to A, chromosome 19 at 6,080,537 bp
  • C to T, chromosome 19 at 8,778,666 bp
  • T to C, chromosome 19 at 8,818,483 bp
  • A to G, chromosome 19 at 8,851,771 bp
  • A to T, chromosome 19 at 12,919,937 bp
  • T to G, chromosome 19 at 17,581,267 bp
  • T to C, chromosome 19 at 18,854,291 bp
  • T to C, chromosome 19 at 58,763,442 bp
Genotype Determination

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6228 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
044357-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.