Strain Name:
C57BL/6J-MtgxR6240Btlr
Stock Number:
044364-MU
Citation ID:
RRID:MMRRC_044364-MU
Other Names:
R6240 (G1)
Major Collection:

Gene Information

Gene Symbol: Cdc25a [MGI:103198] (Mus musculus (mouse))
Name: cell division cycle 25A
Synonyms: D9Ertd393e
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Tnpo1 [MGI:2681523] (Mus musculus (mouse))
Name: transportin 1
Synonyms: D13Ertd688e, Kpnb2
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Pcf11 [MGI:1919579] (Mus musculus (mouse))
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 2500001H09Rik, 5730417B17Rik
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Pepd [MGI:97542] (Mus musculus (mouse))
Name: peptidase D
Synonyms: dal, Pep4, Pep-4, peptidase D
Chromosome: 7
Alteration at locus: Chemically Induced
Gene Symbol: Arhgef18 [MGI:2142567] (Mus musculus (mouse))
Name: rho/rac guanine nucleotide exchange factor (GEF) 18
Synonyms: A430078G23Rik, D030053O22Rik
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Morc3 [MGI:2136841] (Mus musculus (mouse))
Name: microrchidia 3
Synonyms: Zcwcc3, 1110051N18Rik, 1110051N18Rik, D16Jhu32e
Chromosome: 16
Alteration at locus: Chemically Induced
Gene Symbol: Psmd3 [MGI:98858] (Mus musculus (mouse))
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Synonyms: Psd3, Tstap91a, AntP91a
Chromosome: 11
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 182,157,621 bp
  • A to G, chromosome 1 at 189,242,982 bp
  • T to A, chromosome 2 at 24,014,269 bp
  • G to A, chromosome 2 at 36,237,285 bp
  • A to G, chromosome 2 at 88,831,363 bp
  • A to G, chromosome 2 at 89,013,626 bp
  • C to T, chromosome 3 at 32,181,896 bp
  • T to A, chromosome 3 at 64,556,805 bp
  • A to G, chromosome 4 at 118,726,471 bp
  • A to G, chromosome 4 at 133,680,686 bp
  • T to A, chromosome 5 at 31,072,978 bp
  • T to C, chromosome 5 at 57,721,362 bp
  • G to C, chromosome 5 at 84,117,579 bp
  • T to C, chromosome 5 at 104,047,089 bp
  • G to A, chromosome 5 at 125,389,596 bp
  • A to G, chromosome 5 at 149,726,788 bp
  • A to T, chromosome 6 at 52,777,552 bp
  • A to G, chromosome 7 at 4,919,849 bp
  • A to G, chromosome 7 at 35,021,751 bp
  • T to A, chromosome 7 at 92,646,502 bp
  • G to A, chromosome 8 at 3,439,658 bp
  • A to T, chromosome 8 at 10,370,930 bp
  • A to G, chromosome 8 at 13,454,216 bp
  • T to C, chromosome 8 at 36,103,352 bp
  • A to T, chromosome 8 at 67,491,702 bp
  • A to G, chromosome 8 at 95,039,916 bp
  • T to A, chromosome 9 at 21,033,158 bp
  • A to G, chromosome 9 at 22,199,884 bp
  • A to G, chromosome 9 at 40,782,411 bp
  • T to A, chromosome 9 at 71,944,016 bp
  • A to T, chromosome 9 at 106,156,262 bp
  • A to G, chromosome 9 at 109,884,158 bp
  • A to G, chromosome 10 at 20,977,081 bp
  • C to G, chromosome 10 at 80,689,864 bp
  • A to G, chromosome 10 at 122,047,156 bp
  • C to T, chromosome 10 at 128,488,024 bp
  • C to A, chromosome 10 at 129,874,677 bp
  • A to G, chromosome 11 at 98,693,653 bp
  • A to T, chromosome 12 at 87,076,405 bp
  • A to G, chromosome 13 at 22,524,729 bp
  • G to A, chromosome 13 at 50,701,417 bp
  • T to C, chromosome 13 at 98,863,829 bp
  • T to A, chromosome 13 at 110,399,474 bp
  • T to A, chromosome 13 at 110,400,034 bp
  • A to T, chromosome 14 at 24,149,528 bp
  • A to G, chromosome 14 at 50,320,586 bp
  • C to A, chromosome 14 at 63,986,252 bp
  • T to C, chromosome 14 at 65,209,855 bp
  • T to A, chromosome 14 at 87,505,886 bp
  • A to T, chromosome 15 at 4,934,644 bp
  • A to T, chromosome 15 at 11,285,958 bp
  • A to G, chromosome 15 at 23,226,936 bp
  • T to A, chromosome 15 at 102,970,830 bp
  • G to A, chromosome 16 at 73,982,139 bp
  • G to A, chromosome 16 at 85,802,157 bp
  • C to G, chromosome 16 at 93,862,684 bp
  • G to A, chromosome 16 at 96,619,502 bp
  • A to G, chromosome 17 at 24,444,582 bp
  • T to C, chromosome 18 at 6,784,635 bp
  • C to T, chromosome 18 at 22,465,508 bp
  • A to T, chromosome 19 at 9,013,583 bp
  • A to T, chromosome 19 at 29,717,240 bp
  • T to C, chromosome 19 at 56,841,335 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6240 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
044364-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.