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Strain Name:
C57BL/6J-MtgxR6253Btlr/Mmmh
Stock Number:
044370-MU
Citation ID:
RRID:MMRRC_044370-MU
Other Names:
R6253 (G1)
Major Collection:

Strain Information

Cdh3
Name: cadherin 3
Synonyms: P-cadherin, Cadp, Pcad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12560
HGNC: HGNC:1762
Homologene: 20425
Mtss1
Name: MTSS I-BAR domain containing 1
Synonyms: MIM, D130001D01Rik, 2310003N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 211401
VEGA: 15
Homologene: 8841
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Aasdh
Name: aminoadipate-semialdehyde dehydrogenase
Synonyms: A230062G08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231326
Homologene: 71711
Uhmk1
Name: U2AF homology motif (UHM) kinase 1
Synonyms: KIS, Kist, OTTMUSG00000021542, C820018A03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16589
Homologene: 7801
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 170,199,880 bp
  • A to T, chromosome 1 at 176,780,394 bp
  • A to G, chromosome 2 at 19,524,118 bp
  • C to G, chromosome 2 at 75,662,570 bp
  • T to C, chromosome 2 at 114,156,277 bp
  • A to G, chromosome 2 at 154,163,500 bp
  • T to C, chromosome 2 at 157,021,419 bp
  • T to A, chromosome 3 at 38,951,356 bp
  • T to C, chromosome 3 at 94,943,846 bp
  • T to G, chromosome 3 at 98,427,539 bp
  • A to T, chromosome 4 at 15,931,179 bp
  • A to G, chromosome 4 at 32,749,593 bp
  • A to T, chromosome 4 at 135,785,892 bp
  • A to G, chromosome 4 at 135,801,003 bp
  • T to A, chromosome 4 at 155,007,101 bp
  • A to G, chromosome 5 at 41,826,538 bp
  • A to G, chromosome 5 at 76,886,258 bp
  • A to G, chromosome 5 at 88,327,877 bp
  • T to A, chromosome 6 at 68,736,339 bp
  • T to C, chromosome 6 at 116,273,614 bp
  • T to C, chromosome 7 at 6,460,548 bp
  • T to A, chromosome 7 at 26,934,232 bp
  • G to A, chromosome 7 at 107,098,257 bp
  • A to T, chromosome 7 at 107,105,938 bp
  • CCTTCTTCT to CCTTCT, chromosome 7 at 139,638,900 bp
  • T to A, chromosome 8 at 106,537,063 bp
  • C to T, chromosome 8 at 108,955,388 bp
  • T to C, chromosome 9 at 21,500,275 bp
  • G to T, chromosome 9 at 75,056,734 bp
  • A to T, chromosome 9 at 75,245,037 bp
  • G to T, chromosome 9 at 98,490,647 bp
  • C to T, chromosome 9 at 119,428,561 bp
  • T to A, chromosome 10 at 80,233,014 bp
  • G to T, chromosome 11 at 5,280,865 bp
  • A to G, chromosome 11 at 48,894,999 bp
  • C to T, chromosome 11 at 51,574,168 bp
  • G to A, chromosome 11 at 55,296,271 bp
  • A to G, chromosome 11 at 67,301,967 bp
  • G to A, chromosome 11 at 104,357,526 bp
  • A to T, chromosome 11 at 106,426,908 bp
  • A to G, chromosome 12 at 8,748,205 bp
  • A to G, chromosome 12 at 70,877,213 bp
  • A to G, chromosome 12 at 111,835,726 bp
  • T to C, chromosome 13 at 32,972,272 bp
  • T to A, chromosome 13 at 43,094,771 bp
  • T to C, chromosome 13 at 62,454,871 bp
  • A to G, chromosome 13 at 70,603,164 bp
  • C to A, chromosome 13 at 112,636,022 bp
  • T to G, chromosome 13 at 112,636,023 bp
  • A to G, chromosome 14 at 75,719,712 bp
  • A to G, chromosome 15 at 58,943,719 bp
  • T to A, chromosome 15 at 75,911,022 bp
  • A to C, chromosome 16 at 20,639,182 bp
  • T to C, chromosome 16 at 36,915,622 bp
  • T to C, chromosome 16 at 94,457,413 bp
  • G to A, chromosome 17 at 24,397,552 bp
  • A to G, chromosome 17 at 34,970,550 bp
  • T to A, chromosome 17 at 71,231,269 bp
  • T to A, chromosome 17 at 84,740,637 bp
  • A to T, chromosome 19 at 5,892,842 bp
  • G to A, chromosome 19 at 38,124,980 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6253 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044370-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.