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Strain Name:
C57BL/6J-MtgxR6256Btlr/Mmmh
Stock Number:
044373-MU
Citation ID:
RRID:MMRRC_044373-MU
Other Names:
R6256 (G1)
Major Collection:

Strain Information

Dop1b
Name: DOP1 leucine zipper like protein B
Synonyms: 0610038M01Rik, 2610510B01Rik, Dopey2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70028
VEGA: 16
HGNC: HGNC:1291
Homologene: 21068
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Dnmbp
Name: dynamin binding protein
Synonyms: 2410003M15Rik, Tuba, 2410003L07Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71972
Homologene: 9061
Sult6b1
Name: sulfotransferase family, cytosolic, 6B, member 1
Synonyms: 2410078J06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73671
Homologene: 42242
Kdm1a
Name: lysine (K)-specific demethylase 1A
Synonyms: 1810043O07Rik, LSD1, Aof2, Kdm1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 99982
Homologene: 32240
Slc4a2
Name: solute carrier family 4 (anion exchanger), member 2
Synonyms: B3RP, Ae2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20535
Homologene: 128699
Sptlc2
Name: serine palmitoyltransferase, long chain base subunit 2
Synonyms: LCB2, Spt2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20773
Homologene: 21610
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 3 at 69,497,897 bp
  • A to G, chromosome 3 at 93,322,611 bp
  • G to C, chromosome 3 at 137,908,362 bp
  • C to T, chromosome 3 at 137,908,363 bp
  • A to G, chromosome 4 at 126,520,226 bp
  • A to T, chromosome 4 at 134,934,578 bp
  • G to T, chromosome 4 at 136,568,600 bp
  • C to A, chromosome 5 at 24,435,890 bp
  • A to T, chromosome 5 at 96,733,843 bp
  • A to T, chromosome 5 at 104,337,074 bp
  • A to T, chromosome 5 at 121,614,086 bp
  • T to C, chromosome 6 at 18,274,661 bp
  • A to T, chromosome 6 at 30,497,167 bp
  • T to C, chromosome 6 at 34,387,688 bp
  • A to G, chromosome 7 at 80,258,304 bp
  • A to T, chromosome 7 at 99,219,895 bp
  • A to C, chromosome 7 at 99,914,464 bp
  • G to T, chromosome 7 at 126,108,619 bp
  • A to T, chromosome 7 at 141,789,795 bp
  • T to C, chromosome 8 at 71,371,828 bp
  • A to T, chromosome 8 at 72,451,428 bp
  • A to T, chromosome 8 at 93,265,794 bp
  • T to C, chromosome 8 at 128,935,593 bp
  • T to A, chromosome 9 at 58,101,918 bp
  • T to A, chromosome 9 at 107,917,266 bp
  • T to A, chromosome 10 at 67,220,408 bp
  • A to G, chromosome 10 at 80,002,622 bp
  • C to T, chromosome 11 at 69,406,729 bp
  • T to C, chromosome 12 at 87,355,531 bp
  • A to T, chromosome 15 at 26,553,002 bp
  • G to A, chromosome 15 at 47,669,729 bp
  • G to A, chromosome 15 at 89,300,867 bp
  • G to A, chromosome 16 at 4,037,621 bp
  • A to T, chromosome 16 at 57,152,692 bp
  • T to A, chromosome 16 at 93,807,214 bp
  • A to T, chromosome 17 at 57,524,700 bp
  • G to T, chromosome 17 at 65,478,402 bp
  • A to T, chromosome 17 at 78,906,914 bp
  • T to C, chromosome 18 at 31,983,695 bp
  • A to G, chromosome 18 at 37,335,925 bp
  • T to C, chromosome 18 at 37,637,723 bp
  • T to C, chromosome 18 at 78,857,257 bp
  • C to T, chromosome 18 at 80,969,861 bp
  • C to T, chromosome 19 at 43,852,281 bp
  • A to C, chromosome 19 at 60,771,026 bp
  • A to T, chromosome Y at 738,765 bp
  • T to C, chromosome Y at 2,116,267 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6256 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044373-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.