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Strain Name:
C57BL/6J-MtgxR5236Btlr/Mmmh
Stock Number:
044393-MU
Citation ID:
RRID:MMRRC_044393-MU
Other Names:
R5236 (G1)
Major Collection:

Strain Information

Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
H2-DMa
Name: histocompatibility 2, class II, locus DMa
Synonyms: H2-Ma, H-2Ma, H2-M alpha
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14998
HGNC: HGNC:4934
Homologene: 4464
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Arid4b
Name: AT-rich interaction domain 4B
Synonyms: RBBP1L1, SAP180, BRCAA1, Rbp1l1, 6720480E17Rik, 6330417L24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94246
Homologene: 12847
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 34,164,417 bp
  • GT to GTT, chromosome 1 at 88,266,524 bp
  • A to G, chromosome 1 at 128,040,989 bp
  • A to T, chromosome 2 at 32,385,961 bp
  • T to C, chromosome 2 at 69,456,819 bp
  • G to A, chromosome 2 at 76,788,802 bp
  • A to T, chromosome 2 at 101,629,660 bp
  • A to T, chromosome 2 at 127,141,850 bp
  • T to A, chromosome 2 at 132,075,584 bp
  • G to A, chromosome 2 at 164,838,911 bp
  • T to C, chromosome 3 at 33,830,102 bp
  • G to A, chromosome 3 at 92,317,636 bp
  • A to T, chromosome 3 at 99,352,046 bp
  • A to T, chromosome 3 at 139,232,223 bp
  • A to G, chromosome 4 at 24,588,347 bp
  • G to A, chromosome 4 at 44,344,959 bp
  • T to G, chromosome 4 at 102,927,587 bp
  • T to C, chromosome 4 at 104,948,760 bp
  • T to C, chromosome 4 at 123,397,821 bp
  • A to T, chromosome 4 at 156,178,858 bp
  • A to C, chromosome 5 at 114,622,795 bp
  • T to A, chromosome 5 at 114,767,172 bp
  • A to G, chromosome 5 at 144,817,786 bp
  • A to G, chromosome 5 at 146,102,825 bp
  • C to A, chromosome 6 at 41,772,111 bp
  • A to C, chromosome 6 at 42,927,781 bp
  • G to T, chromosome 6 at 83,976,575 bp
  • T to C, chromosome 6 at 134,511,264 bp
  • A to T, chromosome 7 at 13,665,049 bp
  • A to G, chromosome 7 at 19,244,757 bp
  • A to T, chromosome 7 at 85,623,669 bp
  • A to G, chromosome 8 at 85,066,345 bp
  • G to C, chromosome 8 at 104,357,746 bp
  • T to C, chromosome 9 at 21,275,941 bp
  • A to C, chromosome 9 at 57,414,654 bp
  • T to A, chromosome 9 at 67,365,923 bp
  • T to A, chromosome 9 at 101,942,921 bp
  • A to T, chromosome 9 at 123,185,193 bp
  • A to G, chromosome 10 at 60,312,572 bp
  • C to T, chromosome 10 at 110,767,209 bp
  • C to T, chromosome 11 at 50,095,978 bp
  • T to A, chromosome 11 at 82,915,062 bp
  • A to C, chromosome 11 at 99,350,815 bp
  • C to T, chromosome 12 at 8,539,005 bp
  • T to A, chromosome 12 at 103,094,395 bp
  • A to G, chromosome 13 at 14,126,449 bp
  • A to T, chromosome 13 at 62,517,713 bp
  • A to G, chromosome 13 at 113,366,220 bp
  • T to A, chromosome 14 at 54,894,942 bp
  • T to C, chromosome 15 at 100,662,534 bp
  • T to A, chromosome 16 at 8,488,733 bp
  • T to C, chromosome 16 at 22,961,513 bp
  • T to C, chromosome 17 at 32,520,632 bp
  • T to C, chromosome 17 at 33,434,099 bp
  • T to A, chromosome 17 at 34,137,939 bp
  • T to C, chromosome 17 at 67,804,492 bp
  • T to C, chromosome 17 at 78,939,631 bp
  • A to G, chromosome 18 at 36,530,847 bp
  • A to G, chromosome 19 at 9,000,684 bp
  • A to T, chromosome 19 at 36,056,769 bp
  • A to C, chromosome 19 at 38,770,347 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5236 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044393-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.