Strain Name:
C57BL/6J-MtgxR6227Btlr/Mmmh
Stock Number:
044398-MU
Citation ID:
RRID:MMRRC_044398-MU
Other Names:
R6227 (G1)
Major Collection:

Strain Information

Slc26a5
Name: solute carrier family 26, member 5
Synonyms: prestin, Pres
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
Dst
Name: dystonin
Synonyms: Macf2, A830042E19Rik, Bpag1, Bpag, bullous pemphigoid antigen 1, nmf203, athetoid, nmf339, BPAG1, BPAG1-n, 2310001O04Rik, bullous pemphigoid antigen 1, ah
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Pcm1
Name: pericentriolar material 1
Synonyms: C030044G17Rik, 9430077F19Rik, 2600002H09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
Mip
Name: major intrinsic protein of lens fiber
Synonyms: aquaporin 0, Cts, Svl, Aqp0, lens opacity, shrivelled, MIP26, Lop
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17339
HGNC: HGNC:7103
Homologene: 40627
Miga1
Name: mitoguardin 1
Synonyms: C030011O14Rik, Mita1, Fam73a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 215708
Homologene: 18369
Bicd1
Name: BICD cargo adaptor 1
Synonyms: B830009D06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12121
HGNC: HGNC:1049
Homologene: 37518
Inppl1
Name: inositol polyphosphate phosphatase-like 1
Synonyms: SHIP2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16332
HGNC: HGNC:6080
Homologene: 1204
Dab1
Name: disabled 1
Synonyms: C630028C02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13131
HGNC: HGNC:2661
Homologene: 32084
Trf
Name: transferrin
Synonyms: Tfn, HP
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22041
Homologene: 68153
Tlr4
Name: toll-like receptor 4
Synonyms: Lps, lipopolysaccharide response, Rasl2-8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21898
Homologene: 41317
Naca
Name: nascent polypeptide-associated complex alpha polypeptide
Synonyms: LOC380777, skNAC
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17938
VEGA: 10
HGNC: HGNC:7629
Homologene: 136025
Cfap97
Name: cilia and flagella associated protein 97
Synonyms: 1110068E21Rik, 4933411K20Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66756
Homologene: 12026
Rlbp1
Name: retinaldehyde binding protein 1
Synonyms: 3110056M11Rik, CRALBP
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19771
Homologene: 68046
Itga2
Name: integrin alpha 2
Synonyms: DX5, VLA-2 receptor, alpha 2 subunit, CD49B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16398
VEGA: 13
HGNC: HGNC:6137
Homologene: 1662
Abca4
Name: ATP-binding cassette, sub-family A member 4
Synonyms: Rim protein, RmP, D430003I15Rik, Abc10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11304
HGNC: HGNC:34
Homologene: 298
Atp7b
Name: ATPase, Cu++ transporting, beta polypeptide
Synonyms: WND, Wilson protein, Atp7a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11979
HGNC: HGNC:870
Homologene: 20063
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22635
Homologene: 124417
Trim75
Name: tripartite motif-containing 75
Synonyms: LOC333307
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 333307
Homologene: 53452
Pex6
Name: peroxisomal biogenesis factor 6
Synonyms: D130055I09Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224824
HGNC: HGNC:8859
Homologene: 47914
Adgrf1
Name: adhesion G protein-coupled receptor F1
Synonyms: Gpr110, 5031409J19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 77596
Homologene: 124643
Or51f5
Name: olfactory receptor family 51 subfamily F member 5
Synonyms: GA_x6K02T2PBJ9-5491151-5492095, MOR14-2, Olfr561
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259096
Homologene: 133029
Fktn
Name: fukutin
Synonyms: Fukutin, Fcmd
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 246179
HGNC: HGNC:3622
Homologene: 31402
Smtn
Name: smoothelin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 29856
Homologene: 8482
Zfp709
Name: zinc finger protein 709
Synonyms: GIOT-4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 236193
Homologene: 136795
Or6c69
Name: olfactory receptor family 6 subfamily C member 69
Synonyms: GA_x6K02T2PULF-11590830-11589892, MOR113-1, Olfr816
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258667
Homologene: 133584
Rusc1
Name: RUN and SH3 domain containing 1
Synonyms: 2210403N08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72296
Homologene: 75028
4921504E06Rik
Name: RIKEN cDNA 4921504E06 gene
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70909
Homologene: 87049
Or52l1
Name: olfactory receptor family 52 subfamily L member 1
Synonyms: MOR37-1, GA_x6K02T2PBJ9-7810071-7809121, Olfr685
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258160
Homologene: 66455
Rptn
Name: repetin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20129
Homologene: 84780
Csf1r
Name: colony stimulating factor 1 receptor
Synonyms: Fim2, CD115, Fms, Fim-2, M-CSFR, CSF-1R, Csfmr
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12978
HGNC: HGNC:2433
Homologene: 3817
Lactbl1
Name: lactamase, beta-like 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242707
Homologene: 82492
Zfp213
Name: zinc finger protein 213
Synonyms: D17Ertd197e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 449521
VEGA: 17
Homologene: 55809
Tes3-ps
Name: testis derived transcript 3, pseudogene
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 54339
VEGA: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,194,540 bp
  • T to C, chromosome 2 at 19,553,770 bp
  • T to A, chromosome 3 at 89,091,741 bp
  • C to G, chromosome 3 at 93,398,130 bp
  • T to A, chromosome 3 at 122,137,094 bp
  • C to T, chromosome 3 at 152,278,949 bp
  • C to T, chromosome 4 at 53,731,136 bp
  • T to C, chromosome 4 at 66,840,595 bp
  • C to T, chromosome 4 at 104,731,751 bp
  • C to A, chromosome 4 at 136,637,918 bp
  • A to T, chromosome 5 at 21,821,097 bp
  • T to G, chromosome 5 at 137,468,343 bp
  • T to A, chromosome 6 at 149,513,176 bp
  • T to C, chromosome 7 at 79,380,128 bp
  • G to A, chromosome 7 at 101,824,299 bp
  • A to T, chromosome 7 at 102,774,676 bp
  • T to C, chromosome 7 at 105,180,710 bp
  • T to C, chromosome 8 at 22,020,825 bp
  • T to A, chromosome 8 at 41,330,825 bp
  • T to C, chromosome 8 at 46,191,732 bp
  • T to A, chromosome 8 at 64,983,096 bp
  • TCGACG to TCG, chromosome 8 at 71,890,708 bp
  • G to A, chromosome 9 at 103,230,305 bp
  • T to G, chromosome 10 at 128,043,916 bp
  • T to A, chromosome 10 at 128,226,006 bp
  • T to A, chromosome 10 at 129,911,667 bp
  • A to G, chromosome 11 at 3,527,624 bp
  • T to C, chromosome 13 at 49,494,040 bp
  • T to C, chromosome 13 at 92,774,682 bp
  • T to C, chromosome 13 at 114,839,561 bp
  • T to C, chromosome 17 at 23,558,022 bp
  • A to G, chromosome 17 at 43,310,273 bp
  • T to A, chromosome 17 at 46,712,108 bp
  • T to A, chromosome 18 at 61,125,828 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6227 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044398-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.