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Strain Name:
C57BL/6J-MtgxR6294Btlr/Mmmh
Stock Number:
044406-MU
Citation ID:
RRID:MMRRC_044406-MU
Other Names:
R6294 (G1)
Major Collection:

Strain Information

Tnpo1
Name: transportin 1
Synonyms: D13Ertd688e, Kpnb2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 238799
HGNC: HGNC:6401
Homologene: 5358
Omd
Name: osteomodulin
Synonyms: osteoadherin, SLRR2C, OSAD
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27047
VEGA: 13
HGNC: HGNC:8134
Homologene: 3677
Arpp21
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: ARPP-21, Tarpp, D9Bwg1012e, 0710001E13Rik, R3hdm3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74100
Homologene: 32306
Dlg1
Name: discs large MAGUK scaffold protein 1
Synonyms: B130052P05Rik, SAP97, Dlgh1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 13383
HGNC: HGNC:2900
Homologene: 20869
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 160,231,849 bp
  • C to G, chromosome 1 at 188,536,370 bp
  • A to T, chromosome 2 at 24,719,057 bp
  • T to C, chromosome 2 at 80,541,514 bp
  • A to T, chromosome 2 at 103,460,295 bp
  • A to G, chromosome 2 at 110,018,151 bp
  • A to G, chromosome 2 at 112,156,019 bp
  • A to T, chromosome 2 at 117,291,792 bp
  • A to T, chromosome 3 at 95,128,306 bp
  • T to C, chromosome 3 at 144,925,185 bp
  • T to C, chromosome 4 at 108,590,670 bp
  • A to G, chromosome 5 at 34,821,826 bp
  • G to T, chromosome 5 at 63,939,705 bp
  • A to T, chromosome 5 at 73,191,759 bp
  • G to A, chromosome 5 at 121,572,816 bp
  • A to C, chromosome 6 at 49,417,344 bp
  • G to C, chromosome 6 at 57,024,419 bp
  • G to A, chromosome 6 at 69,823,442 bp
  • A to T, chromosome 6 at 121,654,481 bp
  • T to A, chromosome 6 at 148,157,482 bp
  • C to T, chromosome 7 at 4,924,074 bp
  • C to A, chromosome 7 at 18,682,679 bp
  • A to G, chromosome 7 at 29,173,848 bp
  • T to A, chromosome 7 at 43,952,772 bp
  • A to G, chromosome 7 at 45,514,692 bp
  • A to T, chromosome 7 at 103,085,667 bp
  • A to G, chromosome 7 at 103,639,591 bp
  • A to G, chromosome 7 at 135,704,590 bp
  • C to T, chromosome 8 at 5,091,621 bp
  • T to A, chromosome 8 at 85,350,383 bp
  • T to A, chromosome 8 at 110,727,328 bp
  • T to G, chromosome 9 at 7,084,986 bp
  • G to T, chromosome 9 at 65,520,164 bp
  • A to G, chromosome 9 at 96,687,229 bp
  • A to G, chromosome 9 at 110,002,746 bp
  • A to G, chromosome 9 at 112,127,452 bp
  • T to A, chromosome 10 at 39,075,470 bp
  • A to T, chromosome 10 at 60,778,331 bp
  • C to T, chromosome 10 at 79,477,854 bp
  • G to A, chromosome 10 at 93,514,143 bp
  • C to T, chromosome 10 at 127,694,522 bp
  • A to C, chromosome 11 at 90,661,761 bp
  • C to T, chromosome 11 at 93,932,232 bp
  • A to G, chromosome 11 at 94,093,485 bp
  • A to T, chromosome 12 at 5,083,995 bp
  • A to T, chromosome 12 at 113,994,598 bp
  • G to T, chromosome 13 at 11,879,496 bp
  • T to A, chromosome 13 at 49,589,991 bp
  • A to G, chromosome 13 at 50,423,803 bp
  • T to A, chromosome 13 at 98,890,774 bp
  • T to C, chromosome 13 at 103,857,056 bp
  • A to G, chromosome 14 at 20,764,949 bp
  • A to T, chromosome 14 at 38,397,239 bp
  • A to T, chromosome 14 at 84,477,668 bp
  • A to G, chromosome 15 at 4,142,822 bp
  • A to T, chromosome 15 at 44,570,028 bp
  • A to T, chromosome 15 at 75,809,568 bp
  • A to G, chromosome 15 at 84,557,120 bp
  • T to A, chromosome 16 at 31,838,124 bp
  • T to A, chromosome 16 at 89,084,101 bp
  • C to T, chromosome 17 at 6,201,819 bp
  • T to C, chromosome 17 at 20,419,821 bp
  • T to C, chromosome 17 at 37,211,626 bp
  • T to A, chromosome 17 at 37,299,553 bp
  • T to C, chromosome 17 at 46,663,148 bp
  • A to T, chromosome 17 at 71,370,927 bp
  • C to A, chromosome 17 at 74,689,257 bp
  • A to G, chromosome 18 at 60,776,961 bp
  • A to T, chromosome 18 at 76,289,162 bp
  • T to A, chromosome 18 at 78,110,058 bp
  • T to C, chromosome 19 at 13,619,366 bp
  • A to T, chromosome 19 at 59,937,099 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6294 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044406-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.