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Strain Name:
C57BL/6J-MtgxR6312Btlr/Mmmh
Stock Number:
044415-MU
Citation ID:
RRID:MMRRC_044415-MU
Other Names:
R6312 (G1)
Major Collection:

Strain Information

Nudt2
Name: nudix hydrolase 2
Synonyms: APAH1, 2310051L06Rik, nudix (nucleoside diphosphate linked moiety X)-type motif 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66401
HGNC: HGNC:8049
Homologene: 896
Gnai2
Name: G protein subunit alpha i2
Synonyms: Gia, Gnai-2, Galphai2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14678
HGNC: HGNC:4385
Homologene: 55539
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Gng3
Name: guanine nucleotide binding protein (G protein), gamma 3
Synonyms: 83
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14704
VEGA: 19
HGNC: HGNC:4405
Homologene: 22575
Cct2
Name: chaperonin containing TCP1 subunit 2
Synonyms: Cctb
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12461
VEGA: 10
HGNC: HGNC:1615
Homologene: 4696
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Slitrk6
Name: SLIT and NTRK-like family, member 6
Synonyms: 4832410J21Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239250
VEGA: 14
Homologene: 12986
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 23,815,467 bp
  • A to T, chromosome 1 at 93,215,710 bp
  • C to T, chromosome 1 at 139,831,079 bp
  • C to A, chromosome 1 at 190,958,683 bp
  • A to T, chromosome 2 at 36,870,465 bp
  • C to T, chromosome 2 at 69,436,681 bp
  • A to T, chromosome 2 at 86,347,581 bp
  • A to G, chromosome 2 at 126,607,406 bp
  • C to A, chromosome 3 at 94,364,435 bp
  • A to G, chromosome 3 at 158,160,609 bp
  • T to A, chromosome 4 at 11,289,671 bp
  • A to G, chromosome 4 at 41,480,386 bp
  • G to T, chromosome 4 at 46,507,257 bp
  • G to A, chromosome 4 at 120,949,517 bp
  • A to G, chromosome 4 at 134,325,697 bp
  • C to T, chromosome 4 at 138,288,193 bp
  • A to T, chromosome 4 at 144,528,502 bp
  • A to T, chromosome 5 at 27,725,671 bp
  • T to C, chromosome 6 at 48,457,366 bp
  • G to A, chromosome 7 at 5,676,084 bp
  • T to A, chromosome 7 at 12,598,639 bp
  • A to G, chromosome 7 at 26,449,396 bp
  • T to C, chromosome 7 at 27,748,776 bp
  • G to T, chromosome 7 at 29,828,222 bp
  • G to A, chromosome 7 at 30,134,538 bp
  • T to A, chromosome 7 at 81,023,436 bp
  • A to AGGGCGACGG, chromosome 7 at 97,579,904 bp
  • T to A, chromosome 7 at 104,671,589 bp
  • T to C, chromosome 7 at 128,273,543 bp
  • G to A, chromosome 7 at 132,874,965 bp
  • G to T, chromosome 8 at 9,971,739 bp
  • T to C, chromosome 8 at 66,481,466 bp
  • C to A, chromosome 9 at 23,406,791 bp
  • ATTGCTGTTT to ATTGCTGTTTGCTGTTT, chromosome 9 at 38,026,540 bp
  • TTGCTGT to TTGCTGTCTGCTGT, chromosome 9 at 38,026,541 bp
  • GTTT to GTTTGCTGTTTT, chromosome 9 at 38,026,546 bp
  • TTT to TTTGCTGATT, chromosome 9 at 38,026,547 bp
  • T to TGCTGTTC, chromosome 9 at 38,026,549 bp
  • C to T, chromosome 9 at 65,508,061 bp
  • G to T, chromosome 9 at 98,490,647 bp
  • A to T, chromosome 9 at 107,635,117 bp
  • T to A, chromosome 10 at 77,847,127 bp
  • A to G, chromosome 10 at 115,452,924 bp
  • A to T, chromosome 10 at 117,056,055 bp
  • G to A, chromosome 11 at 54,869,990 bp
  • T to A, chromosome 11 at 70,011,767 bp
  • T to A, chromosome 11 at 71,228,397 bp
  • T to C, chromosome 11 at 101,667,253 bp
  • A to C, chromosome 11 at 109,674,630 bp
  • A to G, chromosome 12 at 72,889,767 bp
  • A to G, chromosome 14 at 110,750,247 bp
  • A to G, chromosome 15 at 6,823,638 bp
  • A to G, chromosome 15 at 99,747,115 bp
  • T to A, chromosome 16 at 75,573,915 bp
  • T to C, chromosome 17 at 21,587,167 bp
  • T to C, chromosome 17 at 32,483,294 bp
  • T to C, chromosome 18 at 4,396,175 bp
  • A to G, chromosome 18 at 61,002,385 bp
  • T to A, chromosome 18 at 77,979,211 bp
  • A to G, chromosome 19 at 8,838,633 bp
  • CT to C, chromosome 19 at 55,192,662 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6312 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044415-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.