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Strain Name:
C57BL/6J-MtgxR6235Btlr/Mmmh
Stock Number:
044433-MU
Citation ID:
RRID:MMRRC_044433-MU
Other Names:
R6235 (G1)
Major Collection:

Strain Information

Baiap2
Name: brain-specific angiogenesis inhibitor 1-associated protein 2
Synonyms: IRSp53
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108100
HGNC: HGNC:947
Homologene: 9697
Aldh1l1
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107747
HGNC: HGNC:3978
Homologene: 122031
Itk
Name: IL2 inducible T cell kinase
Synonyms: Emt, Tsk, Tcsk
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Hspa4
Name: heat shock protein 4
Synonyms: APG-2, Hsp70RY, Hsp110, 70kDa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15525
HGNC: HGNC:5237
Homologene: 1624
Dab2ip
Name: disabled 2 interacting protein
Synonyms: 2310011D08Rik, AIP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69601
Homologene: 13058
Hunk
Name: hormonally upregulated Neu-associated kinase
Synonyms: Mak-v, Bstk1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 26559
VEGA: 16
Homologene: 8742
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 74,475,133 bp
  • C to G, chromosome 2 at 28,844,842 bp
  • C to A, chromosome 2 at 29,227,979 bp
  • A to G, chromosome 2 at 35,723,087 bp
  • A to C, chromosome 2 at 65,461,335 bp
  • A to G, chromosome 2 at 85,137,626 bp
  • T to C, chromosome 2 at 86,075,166 bp
  • A to G, chromosome 2 at 119,034,857 bp
  • G to A, chromosome 2 at 120,713,546 bp
  • C to T, chromosome 3 at 104,016,068 bp
  • T to C, chromosome 4 at 32,995,985 bp
  • T to C, chromosome 4 at 43,937,450 bp
  • T to C, chromosome 4 at 81,385,281 bp
  • G to A, chromosome 4 at 120,558,806 bp
  • A to T, chromosome 4 at 138,901,901 bp
  • A to G, chromosome 4 at 140,973,673 bp
  • T to A, chromosome 5 at 86,906,364 bp
  • T to C, chromosome 5 at 134,986,719 bp
  • A to T, chromosome 5 at 142,034,426 bp
  • A to G, chromosome 6 at 30,816,071 bp
  • G to T, chromosome 6 at 48,702,457 bp
  • A to G, chromosome 6 at 66,716,246 bp
  • A to T, chromosome 6 at 90,564,457 bp
  • A to T, chromosome 6 at 121,302,794 bp
  • T to A, chromosome 7 at 13,261,603 bp
  • A to T, chromosome 7 at 25,471,792 bp
  • G to T, chromosome 7 at 29,116,181 bp
  • T to A, chromosome 7 at 43,468,972 bp
  • G to GACGGCGGCT, chromosome 7 at 97,579,909 bp
  • T to G, chromosome 7 at 105,407,699 bp
  • A to G, chromosome 7 at 109,707,413 bp
  • T to G, chromosome 8 at 43,625,912 bp
  • A to T, chromosome 8 at 125,474,871 bp
  • T to G, chromosome 9 at 37,420,929 bp
  • C to T, chromosome 9 at 59,630,815 bp
  • C to A, chromosome 9 at 109,326,190 bp
  • C to T, chromosome 9 at 110,722,316 bp
  • T to C, chromosome 9 at 123,411,880 bp
  • T to A, chromosome 10 at 77,616,237 bp
  • T to A, chromosome 10 at 81,498,882 bp
  • T to A, chromosome 10 at 107,635,338 bp
  • G to A, chromosome 10 at 127,588,177 bp
  • T to C, chromosome 11 at 46,336,428 bp
  • C to T, chromosome 11 at 53,262,939 bp
  • T to C, chromosome 11 at 80,692,944 bp
  • G to A, chromosome 11 at 99,543,094 bp
  • C to T, chromosome 11 at 106,284,054 bp
  • A to T, chromosome 11 at 119,981,408 bp
  • A to G, chromosome 12 at 11,457,516 bp
  • A to T, chromosome 12 at 65,158,408 bp
  • A to T, chromosome 12 at 71,069,772 bp
  • A to T, chromosome 12 at 79,249,599 bp
  • G to T, chromosome 12 at 80,112,822 bp
  • T to A, chromosome 12 at 104,266,532 bp
  • T to C, chromosome 12 at 113,491,710 bp
  • T to A, chromosome 13 at 24,840,395 bp
  • T to C, chromosome 13 at 27,247,945 bp
  • T to C, chromosome 13 at 75,892,910 bp
  • C to A, chromosome 13 at 95,443,850 bp
  • T to A, chromosome 14 at 14,821,113 bp
  • A to T, chromosome 14 at 26,854,804 bp
  • A to T, chromosome 15 at 96,578,792 bp
  • A to T, chromosome 15 at 102,178,606 bp
  • C to A, chromosome 16 at 10,694,635 bp
  • A to G, chromosome 16 at 45,658,122 bp
  • A to G, chromosome 16 at 90,432,706 bp
  • A to G, chromosome 17 at 15,820,819 bp
  • A to G, chromosome 17 at 26,958,130 bp
  • C to A, chromosome 17 at 34,023,306 bp
  • A to G, chromosome 17 at 35,873,877 bp
  • T to C, chromosome 17 at 55,897,427 bp
  • A to G, chromosome 18 at 15,398,113 bp
  • A to G, chromosome 18 at 37,716,430 bp
  • T to A, chromosome 18 at 65,935,161 bp
  • A to G, chromosome 18 at 84,980,904 bp
  • C to A, chromosome 19 at 13,654,781 bp
  • T to C, chromosome 19 at 15,976,486 bp
  • A to G, chromosome 19 at 33,783,563 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6235 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044433-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.