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Strain Name:
C57BL/6J-MtgxR6244Btlr/Mmmh
Stock Number:
044435-MU
Citation ID:
RRID:MMRRC_044435-MU
Other Names:
R6244 (G1)
Major Collection:

Strain Information

Epha2
Name: Eph receptor A2
Synonyms: Sek-2, Eck, Sek2, Myk2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13836
HGNC: HGNC:3386
Homologene: 20929
Ddx50
Name: DExD box helicase 50
Synonyms: GU2, RH-II/Gubeta, 4933429B04Rik, 8430408E17Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 94213
VEGA: 10
Homologene: 56986
Sirt2
Name: sirtuin 2
Synonyms: SIR2L2, Sir2l, 5730427M03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 64383
Homologene: 40823
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Uaca
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: nucling, 2700059D02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72565
VEGA: 9
Homologene: 74297
Setd2
Name: SET domain containing 2
Synonyms: 4921524K10Rik, KMT3A
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235626
Homologene: 56493
Cenpc1
Name: centromere protein C1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12617
HGNC: HGNC:1854
Homologene: 1371
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 40,327,566 bp
  • T to A, chromosome 1 at 59,702,855 bp
  • T to C, chromosome 1 at 88,256,754 bp
  • T to A, chromosome 1 at 107,372,242 bp
  • T to A, chromosome 1 at 117,986,140 bp
  • T to A, chromosome 1 at 133,726,561 bp
  • C to T, chromosome 1 at 175,659,695 bp
  • C to T, chromosome 1 at 177,776,829 bp
  • T to A, chromosome 2 at 36,528,341 bp
  • T to C, chromosome 2 at 54,933,548 bp
  • T to A, chromosome 2 at 63,182,226 bp
  • T to A, chromosome 2 at 86,217,222 bp
  • C to A, chromosome 2 at 92,389,244 bp
  • T to C, chromosome 2 at 156,003,230 bp
  • T to C, chromosome 2 at 168,689,352 bp
  • C to A, chromosome 3 at 28,650,179 bp
  • T to C, chromosome 3 at 36,956,999 bp
  • T to G, chromosome 3 at 89,645,523 bp
  • G to C, chromosome 3 at 104,015,697 bp
  • T to C, chromosome 3 at 108,393,128 bp
  • A to G, chromosome 4 at 99,657,240 bp
  • T to G, chromosome 4 at 118,579,410 bp
  • C to A, chromosome 4 at 126,511,487 bp
  • G to A, chromosome 4 at 141,316,912 bp
  • T to C, chromosome 4 at 156,230,558 bp
  • A to G, chromosome 5 at 27,049,628 bp
  • A to T, chromosome 5 at 30,197,533 bp
  • A to G, chromosome 5 at 36,615,276 bp
  • T to C, chromosome 5 at 52,783,161 bp
  • C to A, chromosome 5 at 86,046,385 bp
  • C to A, chromosome 5 at 121,665,309 bp
  • G to T, chromosome 5 at 150,566,978 bp
  • A to T, chromosome 5 at 151,584,651 bp
  • C to A, chromosome 7 at 16,996,251 bp
  • G to T, chromosome 7 at 28,787,797 bp
  • A to T, chromosome 7 at 44,054,550 bp
  • A to G, chromosome 7 at 45,619,306 bp
  • CG to CGACGGCGGAG, chromosome 7 at 97,579,908 bp
  • A to G, chromosome 7 at 101,259,776 bp
  • G to T, chromosome 7 at 104,487,610 bp
  • G to T, chromosome 7 at 118,634,214 bp
  • C to A, chromosome 7 at 140,113,069 bp
  • C to A, chromosome 7 at 140,638,433 bp
  • T to A, chromosome 7 at 141,237,673 bp
  • G to T, chromosome 8 at 43,138,756 bp
  • T to C, chromosome 9 at 42,255,421 bp
  • G to A, chromosome 9 at 60,870,044 bp
  • G to A, chromosome 9 at 110,548,665 bp
  • C to T, chromosome 9 at 120,051,694 bp
  • A to T, chromosome 10 at 18,140,397 bp
  • A to T, chromosome 10 at 62,621,566 bp
  • T to C, chromosome 10 at 127,508,175 bp
  • C to T, chromosome 10 at 127,657,712 bp
  • A to G, chromosome 11 at 50,814,971 bp
  • A to T, chromosome 11 at 58,684,004 bp
  • A to G, chromosome 11 at 67,362,501 bp
  • G to A, chromosome 11 at 69,744,226 bp
  • A to G, chromosome 11 at 73,145,601 bp
  • A to T, chromosome 11 at 97,213,790 bp
  • A to G, chromosome 11 at 98,770,537 bp
  • C to T, chromosome 11 at 99,235,058 bp
  • A to G, chromosome 11 at 106,376,632 bp
  • A to G, chromosome 12 at 51,610,107 bp
  • C to A, chromosome 12 at 59,206,079 bp
  • A to T, chromosome 12 at 103,764,828 bp
  • A to C, chromosome 13 at 40,861,241 bp
  • A to T, chromosome 13 at 49,530,307 bp
  • A to G, chromosome 13 at 56,773,892 bp
  • G to A, chromosome 13 at 81,106,931 bp
  • A to T, chromosome 13 at 100,152,137 bp
  • A to G, chromosome 14 at 7,892,092 bp
  • A to G, chromosome 14 at 36,940,718 bp
  • T to A, chromosome 14 at 52,463,895 bp
  • G to A, chromosome 14 at 55,868,028 bp
  • T to C, chromosome 14 at 79,086,662 bp
  • G to A, chromosome 16 at 17,652,745 bp
  • C to T, chromosome 16 at 22,932,331 bp
  • A to T, chromosome 17 at 31,239,272 bp
  • T to A, chromosome 17 at 32,906,317 bp
  • T to A, chromosome 17 at 47,672,902 bp
  • T to A, chromosome 18 at 34,793,005 bp
  • A to G, chromosome 19 at 10,200,687 bp
  • A to G, chromosome 19 at 10,625,923 bp
  • G to A, chromosome 19 at 55,070,985 bp
  • G to T, chromosome X at 20,860,100 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6244 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044435-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.