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Strain Name:
C57BL/6J-MtgxR6276Btlr/Mmmh
Stock Number:
044446-MU
Citation ID:
RRID:MMRRC_044446-MU
Other Names:
R6276 (G1)
Major Collection:

Strain Information

Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Cdk11b
Name: cyclin dependent kinase 11B
Synonyms: CDK11-p110, PITSLRE proteins, CDK11-p46, CDK11-p58, Cdc2l2, Cdc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12537
Homologene: 22416
Epb41l2
Name: erythrocyte membrane protein band 4.1 like 2
Synonyms: NBL2, 4.1G, D10Ertd398e, Epb4.1l2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13822
VEGA: 10
HGNC: HGNC:3379
Homologene: 37478
Arhgap39
Name: Rho GTPase activating protein 39
Synonyms: D15Wsu169e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223666
Homologene: 27825
Pck1
Name: phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms: Pck-1, PEPCK
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18534
HGNC: HGNC:8724
Homologene: 1944
Dscaml1
Name: DS cell adhesion molecule like 1
Synonyms: 4930435C18Rik, 4921507G06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114873
VEGA: 9
Homologene: 79549
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 66,399,419 bp
  • C to T, chromosome 1 at 68,560,576 bp
  • A to T, chromosome 1 at 125,395,137 bp
  • T to A, chromosome 1 at 130,418,166 bp
  • C to T, chromosome 1 at 150,738,681 bp
  • T to A, chromosome 1 at 174,218,512 bp
  • T to A, chromosome 2 at 59,948,223 bp
  • A to T, chromosome 2 at 82,980,441 bp
  • A to T, chromosome 2 at 121,323,203 bp
  • T to C, chromosome 2 at 173,157,319 bp
  • G to A, chromosome 2 at 178,279,019 bp
  • A to G, chromosome 3 at 54,318,020 bp
  • T to A, chromosome 3 at 87,800,519 bp
  • A to G, chromosome 3 at 132,743,005 bp
  • A to G, chromosome 4 at 155,634,190 bp
  • A to C, chromosome 5 at 21,301,340 bp
  • A to T, chromosome 5 at 27,761,668 bp
  • C to T, chromosome 5 at 35,280,079 bp
  • A to T, chromosome 5 at 36,604,514 bp
  • A to T, chromosome 5 at 112,811,642 bp
  • GAT to GATCAT, chromosome 6 at 4,756,449 bp
  • A to G, chromosome 6 at 5,431,043 bp
  • G to C, chromosome 6 at 42,629,753 bp
  • T to C, chromosome 6 at 82,743,366 bp
  • T to C, chromosome 7 at 5,490,731 bp
  • A to C, chromosome 7 at 21,260,179 bp
  • A to G, chromosome 7 at 30,506,651 bp
  • A to T, chromosome 7 at 34,242,377 bp
  • A to G, chromosome 7 at 66,306,839 bp
  • A to G, chromosome 7 at 80,875,809 bp
  • A to T, chromosome 7 at 86,921,110 bp
  • T to C, chromosome 7 at 118,434,290 bp
  • C to A, chromosome 7 at 139,921,063 bp
  • T to C, chromosome 8 at 22,939,405 bp
  • T to A, chromosome 8 at 33,577,189 bp
  • C to T, chromosome 8 at 57,536,578 bp
  • C to T, chromosome 8 at 61,513,423 bp
  • T to A, chromosome 8 at 94,623,258 bp
  • A to T, chromosome 8 at 112,752,289 bp
  • C to T, chromosome 9 at 45,668,160 bp
  • C to T, chromosome 9 at 118,049,338 bp
  • G to T, chromosome 10 at 25,502,124 bp
  • A to T, chromosome 10 at 80,026,234 bp
  • T to C, chromosome 10 at 86,744,449 bp
  • T to A, chromosome 10 at 127,089,360 bp
  • G to T, chromosome 11 at 6,395,604 bp
  • C to T, chromosome 11 at 52,376,482 bp
  • T to A, chromosome 11 at 58,446,366 bp
  • A to T, chromosome 11 at 73,799,403 bp
  • CGTTATCTGT to CGT, chromosome 11 at 78,403,480 bp
  • T to C, chromosome 11 at 78,751,230 bp
  • A to T, chromosome 11 at 99,421,902 bp
  • T to C, chromosome 13 at 23,184,295 bp
  • G to T, chromosome 13 at 95,513,938 bp
  • A to G, chromosome 13 at 100,127,995 bp
  • T to C, chromosome 13 at 114,980,852 bp
  • T to C, chromosome 14 at 24,164,568 bp
  • C to T, chromosome 14 at 33,109,525 bp
  • T to A, chromosome 14 at 55,542,624 bp
  • A to G, chromosome 15 at 75,885,700 bp
  • T to C, chromosome 15 at 76,737,536 bp
  • G to T, chromosome 17 at 18,451,470 bp
  • T to A, chromosome 17 at 21,093,983 bp
  • T to G, chromosome 17 at 21,094,690 bp
  • G to A, chromosome 17 at 32,154,749 bp
  • T to C, chromosome 17 at 34,598,754 bp
  • G to A, chromosome 17 at 36,671,710 bp
  • G to A, chromosome 17 at 67,784,088 bp
  • A to G, chromosome 18 at 12,506,949 bp
  • A to G, chromosome 18 at 36,969,767 bp
  • G to A, chromosome 18 at 37,421,760 bp
  • A to G, chromosome 18 at 37,707,644 bp
  • A to G, chromosome 18 at 49,846,586 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6276 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044446-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.