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Strain Name:
C57BL/6J-MtgxR6306Btlr/Mmmh
Stock Number:
044468-MU
Citation ID:
RRID:MMRRC_044468-MU
Other Names:
R6306 (G1)
Major Collection:

Strain Information

Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, semaV, sema5, SemA, LUCA-1, Semaa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20347
Homologene: 20972
Adora2a
Name: adenosine A2a receptor
Synonyms: A2aR, A2AAR, AA2AR, A2a, Rs, ARA2A
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11540
VEGA: 10
HGNC: HGNC:263
Homologene: 20166
Spry2
Name: sprouty RTK signaling antagonist 2
Synonyms: sprouty2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 24064
VEGA: 14
Homologene: 4267
Zfp423
Name: zinc finger protein 423
Synonyms: Roaz, ataxia1, Zfp104, Ebfaz
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94187
Homologene: 9010
Syce2
Name: synaptonemal complex central element protein 2
Synonyms: 1700013H19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71846
Homologene: 12414
Fos
Name: FBJ osteosarcoma oncogene
Synonyms: c-fos, D12Rfj1, cFos
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14281
HGNC: HGNC:3796
Homologene: 3844
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 74,421,311 bp
  • T to C, chromosome 1 at 91,996,174 bp
  • T to C, chromosome 1 at 100,164,146 bp
  • G to T, chromosome 1 at 130,414,066 bp
  • C to T, chromosome 1 at 136,281,199 bp
  • C to T, chromosome 1 at 140,102,417 bp
  • CTGTG to CTG, chromosome 1 at 181,585,024 bp
  • T to C, chromosome 1 at 183,087,148 bp
  • C to G, chromosome 2 at 21,815,611 bp
  • C to T, chromosome 2 at 26,943,887 bp
  • T to G, chromosome 2 at 76,724,110 bp
  • T to A, chromosome 2 at 76,791,920 bp
  • T to C, chromosome 3 at 127,686,316 bp
  • A to T, chromosome 4 at 112,255,875 bp
  • A to T, chromosome 4 at 118,666,293 bp
  • A to G, chromosome 4 at 125,632,412 bp
  • A to G, chromosome 5 at 65,656,296 bp
  • A to G, chromosome 5 at 90,244,154 bp
  • T to C, chromosome 5 at 96,764,946 bp
  • T to C, chromosome 5 at 106,638,124 bp
  • T to C, chromosome 6 at 8,014,969 bp
  • T to C, chromosome 6 at 30,640,954 bp
  • T to A, chromosome 6 at 55,055,824 bp
  • T to C, chromosome 6 at 58,940,254 bp
  • T to C, chromosome 6 at 84,137,266 bp
  • C to A, chromosome 6 at 90,309,278 bp
  • T to C, chromosome 6 at 122,315,162 bp
  • A to G, chromosome 7 at 4,746,481 bp
  • G to A, chromosome 7 at 6,132,361 bp
  • G to T, chromosome 7 at 20,787,867 bp
  • A to C, chromosome 7 at 85,415,591 bp
  • G to A, chromosome 7 at 133,595,325 bp
  • G to A, chromosome 7 at 144,409,680 bp
  • A to G, chromosome 8 at 22,772,266 bp
  • G to T, chromosome 8 at 79,060,695 bp
  • T to A, chromosome 8 at 83,083,502 bp
  • T to C, chromosome 8 at 84,872,742 bp
  • C to A, chromosome 8 at 87,782,034 bp
  • A to G, chromosome 8 at 107,058,423 bp
  • C to T, chromosome 8 at 114,371,651 bp
  • A to G, chromosome 9 at 20,314,446 bp
  • A to T, chromosome 9 at 45,975,127 bp
  • A to G, chromosome 9 at 58,025,100 bp
  • C to T, chromosome 9 at 65,508,061 bp
  • A to G, chromosome 9 at 72,616,955 bp
  • A to T, chromosome 9 at 72,906,186 bp
  • G to A, chromosome 9 at 90,178,278 bp
  • A to G, chromosome 9 at 107,600,920 bp
  • T to A, chromosome 10 at 75,333,404 bp
  • T to C, chromosome 10 at 76,081,351 bp
  • T to A, chromosome 10 at 79,861,732 bp
  • T to A, chromosome 10 at 100,531,166 bp
  • C to A, chromosome 10 at 115,233,243 bp
  • A to T, chromosome 10 at 128,151,181 bp
  • T to C, chromosome 11 at 23,412,260 bp
  • T to G, chromosome 11 at 57,514,146 bp
  • A to G, chromosome 11 at 69,847,646 bp
  • A to G, chromosome 11 at 78,032,693 bp
  • A to G, chromosome 11 at 101,184,615 bp
  • G to A, chromosome 11 at 115,617,560 bp
  • G to T, chromosome 11 at 120,147,819 bp
  • G to A, chromosome 12 at 79,160,549 bp
  • A to T, chromosome 12 at 85,475,686 bp
  • T to A, chromosome 12 at 110,944,751 bp
  • T to A, chromosome 13 at 40,148,576 bp
  • T to A, chromosome 13 at 49,676,353 bp
  • T to C, chromosome 13 at 97,985,388 bp
  • T to A, chromosome 13 at 99,993,577 bp
  • A to C, chromosome 14 at 31,304,587 bp
  • A to T, chromosome 14 at 105,892,984 bp
  • A to T, chromosome 14 at 121,562,080 bp
  • A to G, chromosome 15 at 54,899,346 bp
  • T to C, chromosome 15 at 66,004,794 bp
  • G to A, chromosome 15 at 76,912,085 bp
  • A to G, chromosome 15 at 77,488,961 bp
  • A to T, chromosome 15 at 85,127,623 bp
  • A to G, chromosome 15 at 99,623,953 bp
  • A to G, chromosome 15 at 100,693,424 bp
  • A to G, chromosome 15 at 101,459,523 bp
  • T to C, chromosome 17 at 23,706,150 bp
  • T to C, chromosome 17 at 25,157,528 bp
  • T to G, chromosome 17 at 28,050,639 bp
  • T to A, chromosome 17 at 33,303,186 bp
  • T to C, chromosome 17 at 89,200,533 bp
  • T to C, chromosome 17 at 90,565,446 bp
  • T to A, chromosome 18 at 34,611,251 bp
  • A to T, chromosome 18 at 37,685,913 bp
  • C to A, chromosome 19 at 38,769,465 bp
  • C to T, chromosome X at 74,277,038 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6306 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044468-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.