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Strain Name:
C57BL/6J-MtgxR6315Btlr/Mmmh
Stock Number:
044472-MU
Citation ID:
RRID:MMRRC_044472-MU
Other Names:
R6315 (G1)
Major Collection:

Strain Information

Fgb
Name: fibrinogen beta chain
Synonyms: 2510049G14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 110135
HGNC: HGNC:3662
Homologene: 3772
B4galt5
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5
Synonyms: 9430078I07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56336
HGNC: HGNC:928
Homologene: 3507
Ppp1r1b
Name: protein phosphatase 1, regulatory inhibitor subunit 1B
Synonyms: DARPP-32, Darpp32
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19049
HGNC: HGNC:9287
Homologene: 12972
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Cadm1
Name: cell adhesion molecule 1
Synonyms: 3100001I08Rik, 2900073G06Rik, RA175N, RA175C, RA175B, RA175A, Necl2, SgIGSF, Tslc1, SynCam, Igsf4, Igsf4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54725
HGNC: HGNC:5951
Homologene: 8641
Vsnl1
Name: visinin-like 1
Synonyms: VILIP
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26950
VEGA: 12
Homologene: 2542
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 24,526,452 bp
  • G to A, chromosome 1 at 34,723,477 bp
  • A to G, chromosome 1 at 38,018,033 bp
  • C to G, chromosome 1 at 172,289,336 bp
  • T to C, chromosome 1 at 173,344,352 bp
  • C to T, chromosome 2 at 31,017,758 bp
  • G to T, chromosome 2 at 75,676,819 bp
  • A to G, chromosome 2 at 110,697,039 bp
  • A to T, chromosome 2 at 135,346,341 bp
  • A to G, chromosome 2 at 154,146,076 bp
  • A to T, chromosome 2 at 167,305,809 bp
  • A to G, chromosome 2 at 181,233,202 bp
  • A to G, chromosome 3 at 15,832,306 bp
  • C to T, chromosome 3 at 63,781,390 bp
  • T to A, chromosome 3 at 83,045,055 bp
  • G to A, chromosome 3 at 96,188,186 bp
  • T to A, chromosome 3 at 159,934,371 bp
  • A to T, chromosome 4 at 25,619,774 bp
  • G to A, chromosome 4 at 59,691,132 bp
  • T to A, chromosome 4 at 108,674,488 bp
  • T to C, chromosome 5 at 24,167,504 bp
  • A to G, chromosome 5 at 108,161,582 bp
  • A to T, chromosome 5 at 108,801,891 bp
  • C to T, chromosome 5 at 122,372,575 bp
  • T to A, chromosome 6 at 35,236,869 bp
  • T to C, chromosome 6 at 73,191,605 bp
  • G to A, chromosome 7 at 23,984,758 bp
  • C to T, chromosome 7 at 48,389,235 bp
  • C to T, chromosome 7 at 80,799,890 bp
  • T to C, chromosome 7 at 120,346,092 bp
  • G to A, chromosome 8 at 4,516,980 bp
  • A to G, chromosome 8 at 122,383,865 bp
  • T to C, chromosome 9 at 21,029,952 bp
  • T to C, chromosome 9 at 27,400,837 bp
  • C to T, chromosome 9 at 47,810,119 bp
  • C to A, chromosome 9 at 80,394,074 bp
  • G to T, chromosome 9 at 105,881,970 bp
  • T to C, chromosome 9 at 108,563,439 bp
  • A to T, chromosome 10 at 41,406,841 bp
  • A to T, chromosome 10 at 52,118,210 bp
  • T to G, chromosome 10 at 116,822,819 bp
  • T to C, chromosome 11 at 41,971,861 bp
  • T to A, chromosome 11 at 50,231,764 bp
  • A to G, chromosome 11 at 98,355,390 bp
  • T to A, chromosome 12 at 11,332,155 bp
  • T to C, chromosome 12 at 29,827,798 bp
  • A to T, chromosome 12 at 70,045,615 bp
  • T to G, chromosome 12 at 101,885,578 bp
  • A to G, chromosome 13 at 13,643,504 bp
  • C to T, chromosome 13 at 55,011,157 bp
  • A to G, chromosome 14 at 63,183,471 bp
  • A to G, chromosome 14 at 75,308,915 bp
  • A to G, chromosome 15 at 68,084,462 bp
  • A to C, chromosome 16 at 5,180,277 bp
  • A to C, chromosome 16 at 58,916,246 bp
  • A to C, chromosome 17 at 24,158,356 bp
  • A to T, chromosome 17 at 43,626,338 bp
  • A to G, chromosome 18 at 20,524,586 bp
  • A to G, chromosome 18 at 32,817,090 bp
  • A to T, chromosome 18 at 36,762,069 bp
  • T to C, chromosome 18 at 58,054,953 bp
  • A to T, chromosome 18 at 62,536,147 bp
  • C to A, chromosome 18 at 64,531,479 bp
  • T to C, chromosome 19 at 9,006,626 bp
  • T to C, chromosome 19 at 13,375,405 bp
  • G to A, chromosome 19 at 47,941,277 bp
  • A to G, chromosome 19 at 55,191,472 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6315 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044472-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.