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Strain Name:
C57BL/6J-MtgxR6326Btlr/Mmmh
Stock Number:
044480-MU
Citation ID:
RRID:MMRRC_044480-MU
Other Names:
R6326 (G1)
Major Collection:

Strain Information

Rtn4rl1
Name: reticulon 4 receptor-like 1
Synonyms: Ngr3, Ngrh2, Ngrl2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237847
Homologene: 18611
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Sipa1l1
Name: signal-induced proliferation-associated 1 like 1
Synonyms: 4931426N11Rik, Spar
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217692
VEGA: 12
Homologene: 9189
Mybl1
Name: myeloblastosis oncogene-like 1
Synonyms: A-myb, repro9, G1-419-6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17864
HGNC: HGNC:7547
Homologene: 32045
Map3k8
Name: mitogen-activated protein kinase kinase kinase 8
Synonyms: Cot, Tpl2, c-COT, Tpl-2, Cot/Tpl2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 26410
HGNC: HGNC:6860
Homologene: 3812
Paip1
Name: polyadenylate binding protein-interacting protein 1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218693
Homologene: 4709
Ints14
Name: integrator complex subunit 14
Synonyms: 2010321M09Rik, Vwa9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 69882
VEGA: 9
Homologene: 12774
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 3,671,038 bp
  • T to C, chromosome 1 at 9,678,507 bp
  • T to C, chromosome 1 at 93,076,326 bp
  • A to G, chromosome 1 at 162,649,011 bp
  • T to A, chromosome 1 at 169,987,140 bp
  • A to T, chromosome 1 at 173,729,966 bp
  • T to A, chromosome 1 at 181,783,556 bp
  • C to T, chromosome 2 at 86,850,994 bp
  • G to A, chromosome 2 at 121,139,457 bp
  • T to C, chromosome 2 at 167,484,174 bp
  • C to T, chromosome 3 at 63,781,390 bp
  • A to T, chromosome 3 at 67,399,727 bp
  • A to C, chromosome 3 at 88,912,098 bp
  • A to T, chromosome 3 at 107,500,406 bp
  • A to G, chromosome 3 at 135,239,778 bp
  • A to G, chromosome 4 at 58,073,045 bp
  • T to A, chromosome 4 at 58,827,068 bp
  • G to T, chromosome 4 at 63,324,441 bp
  • T to A, chromosome 4 at 70,235,454 bp
  • T to C, chromosome 4 at 107,914,316 bp
  • C to T, chromosome 4 at 108,478,980 bp
  • T to C, chromosome 4 at 111,880,253 bp
  • A to T, chromosome 4 at 126,316,613 bp
  • C to A, chromosome 4 at 126,565,739 bp
  • T to G, chromosome 4 at 145,269,224 bp
  • A to C, chromosome 5 at 3,962,061 bp
  • A to G, chromosome 5 at 107,406,111 bp
  • A to G, chromosome 5 at 151,046,919 bp
  • T to A, chromosome 6 at 43,346,802 bp
  • T to C, chromosome 6 at 47,866,421 bp
  • T to A, chromosome 6 at 149,328,995 bp
  • G to A, chromosome 7 at 23,984,758 bp
  • C to T, chromosome 7 at 39,408,155 bp
  • C to G, chromosome 7 at 56,222,934 bp
  • A to T, chromosome 7 at 67,120,888 bp
  • G to A, chromosome 7 at 86,801,823 bp
  • A to G, chromosome 7 at 100,416,428 bp
  • C to G, chromosome 7 at 131,543,005 bp
  • A to T, chromosome 8 at 36,102,706 bp
  • A to G, chromosome 8 at 45,942,041 bp
  • G to A, chromosome 8 at 72,341,434 bp
  • A to T, chromosome 8 at 89,030,268 bp
  • A to G, chromosome 8 at 121,805,054 bp
  • T to C, chromosome 9 at 15,279,558 bp
  • T to A, chromosome 9 at 37,427,027 bp
  • T to G, chromosome 9 at 50,764,757 bp
  • T to C, chromosome 9 at 64,964,437 bp
  • A to T, chromosome 10 at 59,428,239 bp
  • A to T, chromosome 10 at 114,567,224 bp
  • A to G, chromosome 10 at 129,570,702 bp
  • T to C, chromosome 11 at 29,819,066 bp
  • T to C, chromosome 11 at 48,889,345 bp
  • T to A, chromosome 11 at 73,131,693 bp
  • C to T, chromosome 11 at 74,666,242 bp
  • T to C, chromosome 11 at 75,266,002 bp
  • T to A, chromosome 11 at 80,155,022 bp
  • C to A, chromosome 11 at 96,287,083 bp
  • T to C, chromosome 11 at 99,416,919 bp
  • A to T, chromosome 12 at 55,747,146 bp
  • A to G, chromosome 12 at 70,045,181 bp
  • A to G, chromosome 12 at 82,372,468 bp
  • A to T, chromosome 12 at 105,661,092 bp
  • A to G, chromosome 13 at 24,840,557 bp
  • A to G, chromosome 13 at 63,543,545 bp
  • A to G, chromosome 13 at 75,132,179 bp
  • A to G, chromosome 13 at 119,430,217 bp
  • T to C, chromosome 14 at 43,182,885 bp
  • T to A, chromosome 14 at 66,303,204 bp
  • A to T, chromosome 14 at 73,198,534 bp
  • T to A, chromosome 15 at 88,994,895 bp
  • A to G, chromosome 15 at 97,245,375 bp
  • A to T, chromosome 15 at 101,850,249 bp
  • G to T, chromosome 16 at 11,403,566 bp
  • C to A, chromosome 17 at 12,612,410 bp
  • T to C, chromosome 17 at 35,836,455 bp
  • T to G, chromosome 17 at 78,919,057 bp
  • C to T, chromosome 18 at 4,340,651 bp
  • T to C, chromosome 18 at 35,680,925 bp
  • T to C, chromosome 18 at 37,722,456 bp
  • C to T, chromosome 19 at 7,096,609 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6326 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044480-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.