Strain Name:
C57BL/6J-MtgxR6336Btlr/Mmmh
Stock Number:
044490-MU
Citation ID:
RRID:MMRRC_044490-MU
Other Names:
R6336 (G1)
Major Collection:

Strain Information

Slc12a5
Name: solute carrier family 12, member 5
Synonyms: KCC2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 57138
Homologene: 10665
Mok
Name: MOK protein kinase
Synonyms: MAPK/MAK/MRK/ overlapping kinase, Stk30, MOK, Rage
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26448
HGNC: HGNC:9833
Homologene: 8062
Depdc5
Name: DEP domain containing 5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 277854
Homologene: 34718
Kdm5a
Name: lysine demethylase 5A
Synonyms: Jarid1a, RBP2, Rbbp2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 214899
HGNC: HGNC:9886
Homologene: 3419
Ankib1
Name: ankyrin repeat and IBR domain containing 1
Synonyms: 2310061P20Rik, 4631416I11Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70797
Homologene: 19076
Rnf17
Name: ring finger protein 17
Synonyms: MMIP-2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 30054
VEGA: 14
Homologene: 23727
Card6
Name: caspase recruitment domain family, member 6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239319
Homologene: 13067
Spg11
Name: SPG11, spatacsin vesicle trafficking associated
Synonyms: spastic paraplegia 11, 6030465E24Rik, C530005A01Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214585
Homologene: 41614
Gabarapl1
Name: GABA type A receptor associated protein like 1
Synonyms: Apg8l, 3110025G09Rik, GECI, 9130422N19Rik, Atg8l
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 57436
HGNC: HGNC:4068
Homologene: 134420
Src
Name: Rous sarcoma oncogene
Synonyms: pp60c-src
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20779
Homologene: 21120
Anapc16
Name: anaphase promoting complex subunit 16
Synonyms: D10Ertd641e, 2310005G07Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52717
Homologene: 11957
2900026A02Rik
Name: RIKEN cDNA 2900026A02 gene
Synonyms: LOC231620, Gm449
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243219
Homologene: 129566
Hoxd4
Name: homeobox D4
Synonyms: Hox-4.2, Hox-5.1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15436
HGNC: HGNC:5138
Homologene: 7773
Fer1l6
Name: fer-1 like family member 6
Synonyms: EG631797
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 631797
Homologene: 53396
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: Mgr1, Mass1, Gpr98, VLGR1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110789
Homologene: 19815
Vwa3a
Name: von Willebrand factor A domain containing 3A
Synonyms: E030013G06Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233813
Homologene: 18607
Clca3a2
Name: chloride channel accessory 3A2
Synonyms: Clca2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80797
HGNC: HGNC:2017
Homologene: 77224
Fbxl13
Name: F-box and leucine-rich repeat protein 13
Synonyms: 4921539K22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320118
Homologene: 27057
Srgap1
Name: SLIT-ROBO Rho GTPase activating protein 1
Synonyms: 4930572H05Rik, Arhgap13
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 117600
Homologene: 56898
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: Adlican2, CMF608, 6530405F15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242050
Homologene: 18712
Kcnt1
Name: potassium channel, subfamily T, member 1
Synonyms: Slack, C030030G16Rik, slo2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227632
Homologene: 11055
Mto1
Name: mitochondrial tRNA translation optimization 1
Synonyms: 2310039H01Rik, 5730419A02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68291
Homologene: 5876
Gabrb1
Name: gamma-aminobutyric acid type A receptor subunit beta 1
Synonyms: Gabrb-1, B230208N19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14400
HGNC: HGNC:4081
Homologene: 20221
Akt3
Name: thymoma viral proto-oncogene 3
Synonyms: Nmf350, D930002M15Rik, PKB gamma
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23797
HGNC: HGNC:393
Homologene: 55904
Map7d1
Name: MAP7 domain containing 1
Synonyms: Mtap7d1, Rprc1, Parcc1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 245877
Homologene: 17009
Ccdc40
Name: coiled-coil domain containing 40
Synonyms: B930008I02Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 207607
Homologene: 27890
Or2y14
Name: olfactory receptor family 2 subfamily Y member 14
Synonyms: Olfr1384, GA_x6K02T2QP88-5922712-5921756, MOR256-23
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258464
Homologene: 74259
Cntnap5b
Name: contactin associated protein-like 5B
Synonyms: Caspr5-2, C230078M14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241175
Homologene: 104295
Whamm
Name: WAS protein homolog associated with actin, golgi membranes and microtubules
Synonyms: Whdc1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434204
Homologene: 45851
Or13c25
Name: olfactory receptor family 13 subfamily C member 25
Synonyms: Olfr272, GA_x6K02T2N78B-7084885-7085844, MOR262-7
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258836
Homologene: 17409
Serpinb9d
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9d
Synonyms: ovalbumin, AT2, Spi9
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20726
HGNC: HGNC:8955
Homologene: 69093
Foxred2
Name: FAD-dependent oxidoreductase domain containing 2
Synonyms: LOC239554, A430097D04Rik, D15Bwg0759e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239554
Homologene: 11800
Phxr2
Name: per-hexamer repeat gene 2
Synonyms: SP28
Type: Gene
Species: Mouse
Chromosome: 10
Ankrd6
Name: ankyrin repeat domain 6
Synonyms: diversin
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 140577
Homologene: 8967
Or52z1
Name: olfactory receptor family 52 subfamily Z member 1
Synonyms: Olfr67, 3'[b]1, MOR31-1, GA_x6K02T2PBJ9-6515150-6514170
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18368
Homologene: 85930
Dlgap1
Name: DLG associated protein 1
Synonyms: 4933422O14Rik, GKAP/SAPAP, D17Bwg0511e, SAPAP1, DAP-1 beta, Gkap, Sapap1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224997
HGNC: HGNC:2905
Homologene: 31258
Grin3b
Name: glutamate receptor, ionotropic, NMDA3B
Synonyms: NR3B
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 170483
Homologene: 15606
Or2f1b
Name: olfactory receptor family 2 subfamily F member 1B
Synonyms: 18A, MOR257-2, GA_x6K02T2P3E9-4797841-4796888, Olfr38
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258988
HGNC: HGNC:8246
Homologene: 72050
Gbp11
Name: guanylate binding protein 11
Synonyms: Gm7141
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 634650
Homologene: 128731
Ncf2
Name: neutrophil cytosolic factor 2
Synonyms: NADPH oxidase subunit (67kDa), p67phox, Ncf-2, NOXA2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17970
HGNC: HGNC:7661
Homologene: 374
Ephb4
Name: Eph receptor B4
Synonyms: Tyro11, b2b2412Clo, MDK2, Htk, Myk1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13846
HGNC: HGNC:3395
Homologene: 20939
Or4k49
Name: olfactory receptor family 4 subfamily K member 49
Synonyms: MOR248-8, GA_x6K02T2Q125-72715642-72716580, Olfr1299
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258886
Homologene: 17426
Mtss2
Name: MTSS I-BAR domain containing 2
Synonyms: Mtss1l, ABBA
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244654
Homologene: 70926
Ccdc152
Name: coiled-coil domain containing 152
Synonyms: ENSMUSG00000071814
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 100039139
VEGA: 15
Homologene: 79247
Slc22a6
Name: solute carrier family 22 (organic anion transporter), member 6
Synonyms: NKT, mOat1, Oat1, Orctl1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18399
VEGA: 19
Homologene: 16813
Or4f47
Name: olfactory receptor family 4 subfamily F member 47
Synonyms: GA_x6K02T2Q125-73188162-73189112, MOR245-4, Olfr1317
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258440
Homologene: 71983
Vmn2r30
Name: vomeronasal 2, receptor 30
Synonyms: V2r15
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22306
Homologene: 113703
Ddo
Name: D-aspartate oxidase
Synonyms: 5730402C02Rik, 5330420D20Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70503
VEGA: 10
HGNC: HGNC:2727
Homologene: 101531
Potefam3f
Name: POTE ankyrin domain family member 3F
Synonyms: Pote3f, Gm31371
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 78672
Homologene: 128726
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 100,358,669 bp
  • T to C, chromosome 1 at 152,834,070 bp
  • T to C, chromosome 1 at 177,031,712 bp
  • A to G, chromosome 2 at 25,888,755 bp
  • A to G, chromosome 2 at 74,727,361 bp
  • A to G, chromosome 2 at 111,664,619 bp
  • A to C, chromosome 2 at 112,142,406 bp
  • G to C, chromosome 2 at 122,112,959 bp
  • T to A, chromosome 2 at 157,457,155 bp
  • T to A, chromosome 2 at 164,992,464 bp
  • G to A, chromosome 3 at 59,330,339 bp
  • G to T, chromosome 3 at 144,806,478 bp
  • A to G, chromosome 4 at 32,860,411 bp
  • A to T, chromosome 4 at 52,911,459 bp
  • A to G, chromosome 4 at 126,236,682 bp
  • A to T, chromosome 5 at 3,700,377 bp
  • A to G, chromosome 5 at 21,523,547 bp
  • T to C, chromosome 5 at 32,964,507 bp
  • A to C, chromosome 5 at 72,029,898 bp
  • A to G, chromosome 5 at 105,325,489 bp
  • G to T, chromosome 5 at 113,183,483 bp
  • T to A, chromosome 5 at 137,372,085 bp
  • T to C, chromosome 6 at 42,762,657 bp
  • C to A, chromosome 6 at 120,398,978 bp
  • A to G, chromosome 6 at 129,537,528 bp
  • T to A, chromosome 7 at 7,334,308 bp
  • T to A, chromosome 7 at 81,591,764 bp
  • T to C, chromosome 7 at 103,788,245 bp
  • A to G, chromosome 7 at 120,762,423 bp
  • A to G, chromosome 8 at 19,924,350 bp
  • G to A, chromosome 8 at 110,732,164 bp
  • A to T, chromosome 9 at 78,473,835 bp
  • T to C, chromosome 10 at 40,633,031 bp
  • A to T, chromosome 10 at 59,988,829 bp
  • C to A, chromosome 10 at 79,976,461 bp
  • T to A, chromosome 10 at 99,126,090 bp
  • A to T, chromosome 10 at 121,925,941 bp
  • G to T, chromosome 11 at 49,514,542 bp
  • A to G, chromosome 11 at 119,231,993 bp
  • A to G, chromosome 12 at 110,834,124 bp
  • T to C, chromosome 13 at 33,194,694 bp
  • T to C, chromosome 13 at 81,385,981 bp
  • A to G, chromosome 14 at 56,421,169 bp
  • T to C, chromosome 15 at 3,301,129 bp
  • T to A, chromosome 15 at 5,099,164 bp
  • C to A, chromosome 15 at 58,559,232 bp
  • A to G, chromosome 15 at 77,955,764 bp
  • T to A, chromosome 17 at 70,815,289 bp
  • G to A, chromosome 19 at 8,622,130 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6336 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044490-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.