Strain Name:
C57BL/6J-MtgxR6354Btlr/Mmmh
Stock Number:
044506-MU
Citation ID:
RRID:MMRRC_044506-MU
Other Names:
R6354 (G1)
Major Collection:

Strain Information

Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: bt, ADAMTS-20
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Meis1
Name: Meis homeobox 1
Synonyms: C530044H18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17268
HGNC: HGNC:7000
Homologene: 86803
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: p110beta, 1110001J02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Phf21a
Name: PHD finger protein 21A
Synonyms: 80kDa, D030065N23Rik, Braf35/HDAC complex (Bhc), PFTF1, Bhc80
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192285
Homologene: 9597
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: 2410098H20Rik, D5Bwg1524e, Rutbc2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52850
Homologene: 64485
Ube3c
Name: ubiquitin protein ligase E3C
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100763
Homologene: 8783
Zeb1
Name: zinc finger E-box binding homeobox 1
Synonyms: [delta]EF1, AREB6, Zfx1a, Zfhx1a, Nil2, ZEB, Tcf18, Tcf8, MEB1, 3110032K11Rik, Tw, Zfhep
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 21417
Homologene: 31779
Txlna
Name: taxilin alpha
Synonyms: 2600010N21Rik, IL14, Txln
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109658
Homologene: 14062
Zfp619
Name: zinc finger protein 619
Synonyms: 3000002G13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 70227
Homologene: 133818
Cdc27
Name: cell division cycle 27
Synonyms: APC3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217232
HGNC: HGNC:1728
Homologene: 960
Trak1
Name: trafficking protein, kinesin binding 1
Synonyms: 2310001H13Rik, hyrt
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67095
Homologene: 25103
Robo3
Name: roundabout guidance receptor 3
Synonyms: Rbig1, Robo3a, Robo3b, Rig1, Rig-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19649
Homologene: 32119
Atad3a
Name: ATPase family, AAA domain containing 3A
Synonyms: Tob3, 2400004H09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 108888
Homologene: 10059
Casz1
Name: castor zinc finger 1
Synonyms: castor, 2410019P08Rik, D4Ertd432e, Cst
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69743
Homologene: 9824
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: Min, adenomatosis polyposis coli, CC1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Zfp644
Name: zinc finger protein 644
Synonyms: Zep-2, 1110068L01Rik, BM-005, D5Ertd689e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 52397
Homologene: 12971
Lrrc40
Name: leucine rich repeat containing 40
Synonyms: 2610040E16Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67144
Homologene: 9825
Cwf19l1
Name: CWF19 like cell cycle control factor 1
Synonyms: 2610528C06Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72502
VEGA: 19
Homologene: 41244
Clstn1
Name: calsyntenin 1
Synonyms: calsyntenin-1, 1810034E21Rik, Cst-1, alcadein alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 65945
Homologene: 8814
Tnrc6c
Name: trinucleotide repeat containing 6C
Synonyms: 9930033H14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217351
Homologene: 23137
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh-4, Zfh4, C130041O22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80892
Homologene: 23477
Ciita
Name: class II transactivator
Synonyms: Gm9475, C2ta
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12265
VEGA: 16
HGNC: HGNC:7067
Homologene: 207
Lama2
Name: laminin, alpha 2
Synonyms: merosin, mer
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237954
Homologene: 138824
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
Cimap1a
Name: ciliary microtubule associated protein 1A
Synonyms: 1700011O04Rik, SHIPPO1, Odf3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69287
Homologene: 12306
Cep126
Name: centrosomal protein 126
Synonyms: AK129341
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 234915
VEGA: 9
Homologene: 28313
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 214321
Homologene: 86950
Cfap251
Name: cilia and flagella associated protein 251
Synonyms: 4930415N18Rik, 4933428F06Rik, Wdr66
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269701
Homologene: 16964
Vmn2r72
Name: vomeronasal 2, receptor 72
Synonyms: Vmn2r72-ps, EG244114
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244114
Homologene: 115466
Crybg2
Name: crystallin beta-gamma domain containing 2
Synonyms: Aim1l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230806
Homologene: 19232
Mpo
Name: myeloperoxidase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17523
HGNC: HGNC:7218
Homologene: 55450
Or4c102
Name: olfactory receptor family 4 subfamily C member 102
Synonyms: GA_x6K02T2Q125-50079044-50079964, Olfr1189, MOR237-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258768
Homologene: 81569
Phldb2
Name: pleckstrin homology like domain, family B, member 2
Synonyms: C820004H04Rik, LL5b, LL5beta
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208177
Homologene: 17100
Tmc6
Name: transmembrane channel-like gene family 6
Synonyms: EVER1, D11Ertd204e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217353
Homologene: 5258
Fam98c
Name: family with sequence similarity 98, member C
Synonyms: 1110006G06Rik, B230110F21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73833
Homologene: 45483
Or56a5
Name: olfactory receptor family 56 subfamily A member 5
Synonyms: MOR40-1, Olfr683, GA_x6K02T2PBJ9-7773007-7772066
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259047
Homologene: 133645
Iqce
Name: IQ motif containing E
Synonyms: 1700028P05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 74239
Homologene: 49912
Ivl
Name: involucrin
Synonyms: 1110019C06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16447
HGNC: HGNC:6187
Homologene: 137207
Vps36
Name: vacuolar protein sorting 36
Synonyms: Eap45, 2210415M20Rik, 1700010A24Rik, 2810408E15Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 70160
Homologene: 9374
Cpb2
Name: carboxypeptidase B2
Synonyms: carboxypeptidase R, thrombin-activatable fibrinolysis inhibitor, CPU, carboxypeptidase U, TAFI, CPR, 1110032P04Rik, 4930405E17Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56373
VEGA: 14
HGNC: HGNC:2300
Homologene: 55610
Slc5a7
Name: solute carrier family 5 (choline transporter), member 7
Synonyms: CHT1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 63993
VEGA: 17
Homologene: 32516
Vmn2r65
Name: vomeronasal 2, receptor 65
Synonyms: ENSMUSG00000070600
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100009609
Inpp1
Name: inositol polyphosphate-1-phosphatase
Synonyms: 2300002C06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16329
HGNC: HGNC:6071
Homologene: 1655
Ccdc62
Name: coiled-coil domain containing 62
Synonyms: LOC208908, repro29, G1-485-3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 208908
Homologene: 82466
Zbtb49
Name: zinc finger and BTB domain containing 49
Synonyms: 4930518A03Rik, Zfp509
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75079
Homologene: 34825
Or2y1b
Name: olfactory receptor family 2 subfamily Y member 1B
Synonyms: Olfr10, GA_x6K02T2QP88-6117098-6116163, MOR256-55, L45
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18307
Homologene: 81347
Mtmr11
Name: myotubularin related protein 11
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 194126
Homologene: 82382
Cyp4a14
Name: cytochrome P450, family 4, subfamily a, polypeptide 14
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13119
Homologene: 134697
Gcsh
Name: glycine cleavage system protein H (aminomethyl carrier)
Synonyms: 1100001L02Rik, 5730591C18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 68133
HGNC: HGNC:4208
Homologene: 90880
Gimap4
Name: GTPase, IMAP family member 4
Synonyms: E430007K16Rik, Ian1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107526
Homologene: 75084
Krtap13
Name: keratin associated protein 13
Synonyms: 4C32
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16699
VEGA: 16
Homologene: 114409
Krtap10-25
Name: keratin associated protein 10-25
Synonyms: Gm7137
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 634504
VEGA: 10
Homologene: 115744
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 52,797,065 bp
  • T to A, chromosome 2 at 88,592,134 bp
  • G to A, chromosome 2 at 92,348,937 bp
  • T to C, chromosome 3 at 5,401,951 bp
  • CCTGCTGCTGCTGCT to CCTGCTGCTGCT, chromosome 3 at 92,571,910 bp
  • T to A, chromosome 3 at 96,168,676 bp
  • T to A, chromosome 3 at 158,061,264 bp
  • A to G, chromosome 4 at 115,487,244 bp
  • A to T, chromosome 4 at 129,634,412 bp
  • A to G, chromosome 4 at 134,091,136 bp
  • G to T, chromosome 4 at 148,952,542 bp
  • A to C, chromosome 4 at 149,643,216 bp
  • A to G, chromosome 4 at 155,753,945 bp
  • T to C, chromosome 5 at 29,663,583 bp
  • T to C, chromosome 5 at 38,203,559 bp
  • A to G, chromosome 5 at 106,636,753 bp
  • T to G, chromosome 5 at 113,282,656 bp
  • C to T, chromosome 5 at 123,302,755 bp
  • C to T, chromosome 5 at 123,944,204 bp
  • T to C, chromosome 5 at 140,676,335 bp
  • G to A, chromosome 6 at 48,590,334 bp
  • T to C, chromosome 6 at 48,686,880 bp
  • C to T, chromosome 7 at 29,152,847 bp
  • C to A, chromosome 7 at 39,534,819 bp
  • T to A, chromosome 7 at 84,940,366 bp
  • A to G, chromosome 7 at 85,750,539 bp
  • G to A, chromosome 7 at 105,143,708 bp
  • A to G, chromosome 7 at 140,850,614 bp
  • T to C, chromosome 8 at 22,205,755 bp
  • T to C, chromosome 8 at 33,343,638 bp
  • T to C, chromosome 8 at 116,983,843 bp
  • A to G, chromosome 9 at 8,099,927 bp
  • A to T, chromosome 9 at 37,417,217 bp
  • G to T, chromosome 9 at 99,073,643 bp
  • A to T, chromosome 9 at 121,451,726 bp
  • G to T, chromosome 10 at 27,212,068 bp
  • C to A, chromosome 10 at 77,787,647 bp
  • A to T, chromosome 11 at 19,016,184 bp
  • A to T, chromosome 11 at 49,317,638 bp
  • T to A, chromosome 11 at 87,797,346 bp
  • T to A, chromosome 11 at 103,464,387 bp
  • C to A, chromosome 11 at 104,534,748 bp
  • T to C, chromosome 11 at 109,794,216 bp
  • A to T, chromosome 11 at 117,749,614 bp
  • A to C, chromosome 11 at 117,774,236 bp
  • C to T, chromosome 12 at 81,377,981 bp
  • T to C, chromosome 14 at 75,257,705 bp
  • C to T, chromosome 15 at 47,881,489 bp
  • C to A, chromosome 15 at 94,347,810 bp
  • A to T, chromosome 16 at 10,523,746 bp
  • T to C, chromosome 16 at 32,754,358 bp
  • T to C, chromosome 16 at 45,825,114 bp
  • C to A, chromosome 16 at 88,751,243 bp
  • A to G, chromosome 17 at 54,277,033 bp
  • A to G, chromosome 18 at 5,772,743 bp
  • A to T, chromosome 18 at 34,312,528 bp
  • G to T, chromosome 19 at 44,127,473 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6354 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044506-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.