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Strain Name:
C57BL/6J-MtgxR6364Btlr/Mmmh
Stock Number:
044514-MU
Citation ID:
RRID:MMRRC_044514-MU
Other Names:
R6364 (G1)
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Blm
Name: Bloom syndrome, RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12144
HGNC: HGNC:1058
Homologene: 47902
Cops3
Name: COP9 signalosome subunit 3
Synonyms: Sgn3, COP9 complex S3, Csn3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26572
HGNC: HGNC:2239
Homologene: 2710
Epop
Name: elongin BC and polycomb repressive complex 2 associated protein
Synonyms: E13, E130012A19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103551
Homologene: 82351
Wdr43
Name: WD repeat domain 43
Synonyms: 2610318G08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72515
VEGA: 17
Homologene: 38810
Ambra1
Name: autophagy/beclin 1 regulator 1
Synonyms: 2310079H06Rik, D030051N19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228361
Homologene: 18204
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 58,658,372 bp
  • T to A, chromosome 1 at 174,042,212 bp
  • G to T, chromosome 2 at 52,107,669 bp
  • T to A, chromosome 2 at 68,118,672 bp
  • T to C, chromosome 2 at 72,970,941 bp
  • C to A, chromosome 2 at 91,773,316 bp
  • T to C, chromosome 2 at 120,713,429 bp
  • T to A, chromosome 2 at 121,096,397 bp
  • T to C, chromosome 2 at 155,834,523 bp
  • G to T, chromosome 2 at 158,462,109 bp
  • T to C, chromosome 2 at 158,783,259 bp
  • C to A, chromosome 2 at 168,912,266 bp
  • T to C, chromosome 3 at 88,365,958 bp
  • G to A, chromosome 3 at 97,587,167 bp
  • A to T, chromosome 3 at 119,740,442 bp
  • G to A, chromosome 3 at 129,872,846 bp
  • C to T, chromosome 3 at 146,624,577 bp
  • T to A, chromosome 4 at 43,643,622 bp
  • T to C, chromosome 4 at 109,961,800 bp
  • A to G, chromosome 5 at 23,540,467 bp
  • A to G, chromosome 5 at 23,844,030 bp
  • T to C, chromosome 5 at 25,309,636 bp
  • G to A, chromosome 5 at 31,088,739 bp
  • T to A, chromosome 5 at 65,453,296 bp
  • T to C, chromosome 5 at 89,721,814 bp
  • G to T, chromosome 5 at 107,842,113 bp
  • T to C, chromosome 5 at 120,460,609 bp
  • T to C, chromosome 5 at 120,553,810 bp
  • G to T, chromosome 5 at 141,962,709 bp
  • C to A, chromosome 6 at 83,053,046 bp
  • G to A, chromosome 6 at 116,043,761 bp
  • A to G, chromosome 6 at 121,779,443 bp
  • C to T, chromosome 6 at 136,932,255 bp
  • A to T, chromosome 6 at 139,500,748 bp
  • C to A, chromosome 7 at 25,272,823 bp
  • T to A, chromosome 7 at 35,203,709 bp
  • T to C, chromosome 7 at 45,858,454 bp
  • A to T, chromosome 7 at 80,494,526 bp
  • G to A, chromosome 7 at 102,176,315 bp
  • T to C, chromosome 7 at 104,336,526 bp
  • C to T, chromosome 7 at 110,821,309 bp
  • A to T, chromosome 7 at 142,175,063 bp
  • A to G, chromosome 7 at 144,410,409 bp
  • A to G, chromosome 7 at 144,919,268 bp
  • G to A, chromosome 8 at 71,599,089 bp
  • A to G, chromosome 8 at 79,646,341 bp
  • A to G, chromosome 8 at 107,368,277 bp
  • G to T, chromosome 8 at 121,604,859 bp
  • A to G, chromosome 9 at 15,863,004 bp
  • A to T, chromosome 9 at 103,448,628 bp
  • G to A, chromosome 9 at 119,121,871 bp
  • T to C, chromosome 10 at 80,710,494 bp
  • T to C, chromosome 10 at 121,294,725 bp
  • A to T, chromosome 11 at 29,188,035 bp
  • A to G, chromosome 11 at 59,835,404 bp
  • C to T, chromosome 11 at 70,947,786 bp
  • A to G, chromosome 11 at 75,436,489 bp
  • A to G, chromosome 11 at 97,628,687 bp
  • G to A, chromosome 11 at 101,630,242 bp
  • T to C, chromosome 12 at 99,388,693 bp
  • T to C, chromosome 12 at 103,654,236 bp
  • A to T, chromosome 12 at 116,241,732 bp
  • C to T, chromosome 13 at 54,524,600 bp
  • C to A, chromosome 14 at 20,713,011 bp
  • A to G, chromosome 15 at 77,638,058 bp
  • T to C, chromosome 15 at 99,406,185 bp
  • G to A, chromosome 16 at 90,260,248 bp
  • A to G, chromosome 16 at 97,413,433 bp
  • A to G, chromosome 17 at 20,470,998 bp
  • A to T, chromosome 17 at 49,620,623 bp
  • A to G, chromosome 17 at 71,657,654 bp
  • T to C, chromosome 18 at 44,276,511 bp
  • T to G, chromosome 18 at 68,407,536 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6364 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044514-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.