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Strain Name:
C57BL/6J-MtgxR6374Btlr/Mmmh
Stock Number:
044524-MU
Citation ID:
RRID:MMRRC_044524-MU
Other Names:
R6374 (G1)
Major Collection:

Strain Information

Atic
Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Synonyms: 2610509C24Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108147
HGNC: HGNC:794
Homologene: 2983
Dnmt1
Name: DNA methyltransferase 1
Synonyms: MTase, Dnmt1o, Cxxc9, MommeD2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Sult6b1
Name: sulfotransferase family, cytosolic, 6B, member 1
Synonyms: 2410078J06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73671
Homologene: 42242
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Nup35
Name: nucleoporin 35
Synonyms: 5330402E05Rik, 2310006I24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 69482
Homologene: 44517
Hnrnpll
Name: heterogeneous nuclear ribonucleoprotein L-like
Synonyms: 2510028H02Rik, 2810036L13Rik, Hnrpll
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72692
Homologene: 26701
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 15,883,663 bp
  • T to C, chromosome 1 at 58,172,161 bp
  • C to T, chromosome 1 at 71,564,941 bp
  • T to A, chromosome 1 at 116,822,291 bp
  • T to A, chromosome 1 at 158,956,645 bp
  • T to C, chromosome 1 at 163,949,214 bp
  • C to T, chromosome 1 at 172,289,375 bp
  • T to A, chromosome 1 at 174,214,168 bp
  • T to C, chromosome 1 at 181,923,580 bp
  • T to C, chromosome 1 at 193,182,010 bp
  • A to G, chromosome 2 at 37,603,008 bp
  • T to C, chromosome 2 at 39,034,068 bp
  • G to T, chromosome 2 at 80,658,386 bp
  • G to C, chromosome 2 at 127,431,918 bp
  • T to G, chromosome 2 at 134,523,104 bp
  • A to C, chromosome 2 at 151,472,880 bp
  • T to C, chromosome 2 at 155,421,156 bp
  • A to T, chromosome 2 at 158,272,054 bp
  • T to C, chromosome 2 at 181,715,928 bp
  • A to G, chromosome 3 at 5,244,035 bp
  • C to T, chromosome 3 at 130,620,693 bp
  • A to T, chromosome 3 at 142,738,237 bp
  • T to C, chromosome 4 at 145,122,681 bp
  • T to C, chromosome 4 at 147,674,842 bp
  • T to A, chromosome 4 at 155,785,764 bp
  • T to C, chromosome 5 at 22,080,714 bp
  • C to T, chromosome 5 at 29,225,268 bp
  • C to T, chromosome 5 at 44,055,983 bp
  • A to T, chromosome 5 at 87,242,420 bp
  • G to A, chromosome 5 at 109,053,813 bp
  • T to A, chromosome 6 at 37,953,549 bp
  • A to T, chromosome 6 at 124,732,569 bp
  • A to T, chromosome 6 at 131,552,929 bp
  • C to A, chromosome 7 at 12,615,493 bp
  • C to T, chromosome 7 at 65,980,155 bp
  • T to C, chromosome 7 at 87,002,290 bp
  • A to G, chromosome 7 at 100,341,065 bp
  • A to G, chromosome 7 at 103,611,921 bp
  • C to T, chromosome 7 at 104,390,402 bp
  • T to G, chromosome 7 at 109,486,062 bp
  • A to G, chromosome 7 at 140,835,211 bp
  • T to C, chromosome 8 at 22,565,652 bp
  • A to G, chromosome 8 at 33,575,912 bp
  • T to C, chromosome 8 at 69,659,344 bp
  • C to T, chromosome 8 at 71,641,453 bp
  • A to T, chromosome 8 at 105,841,540 bp
  • A to T, chromosome 8 at 125,444,630 bp
  • A to T, chromosome 9 at 20,924,045 bp
  • G to A, chromosome 9 at 45,009,363 bp
  • C to A, chromosome 9 at 56,257,666 bp
  • A to G, chromosome 9 at 57,117,481 bp
  • T to A, chromosome 9 at 76,492,907 bp
  • C to T, chromosome 10 at 4,549,746 bp
  • T to A, chromosome 10 at 82,288,897 bp
  • T to C, chromosome 10 at 93,856,310 bp
  • T to C, chromosome 10 at 127,707,680 bp
  • T to C, chromosome 11 at 23,438,914 bp
  • C to T, chromosome 11 at 62,156,592 bp
  • T to C, chromosome 11 at 69,736,932 bp
  • T to C, chromosome 11 at 102,146,511 bp
  • A to T, chromosome 11 at 110,083,390 bp
  • T to C, chromosome 12 at 5,018,002 bp
  • T to A, chromosome 12 at 11,305,873 bp
  • A to G, chromosome 14 at 7,974,115 bp
  • T to A, chromosome 14 at 32,058,159 bp
  • A to G, chromosome 15 at 59,316,110 bp
  • A to T, chromosome 15 at 59,337,195 bp
  • A to G, chromosome 15 at 76,120,457 bp
  • A to T, chromosome 15 at 78,847,449 bp
  • A to G, chromosome 16 at 22,960,992 bp
  • T to C, chromosome 16 at 33,727,129 bp
  • T to C, chromosome 16 at 97,950,368 bp
  • G to T, chromosome 17 at 34,804,136 bp
  • A to G, chromosome 17 at 35,660,045 bp
  • A to G, chromosome 17 at 56,511,702 bp
  • G to T, chromosome 17 at 78,906,931 bp
  • A to T, chromosome 17 at 80,049,874 bp
  • T to A, chromosome 19 at 59,038,296 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6374 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044524-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.