Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6404Btlr/Mmmh
Stock Number:
044549-MU
Citation ID:
RRID:MMRRC_044549-MU
Other Names:
R6404 (G1)
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Uba2
Name: ubiquitin-like modifier activating enzyme 2
Synonyms: Sumo-1 activating enzyme subunit 2, UBA2, anthracycline-associated resistance, Arx, SAE2, Uble1b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50995
Homologene: 4018
Frmd5
Name: FERM domain containing 5
Synonyms: 1500032A09Rik, A930004K21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228564
Homologene: 18206
Iqgap2
Name: IQ motif containing GTPase activating protein 2
Synonyms: 4933417J23Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 544963
VEGA: 13
HGNC: HGNC:6111
Homologene: 101543
Rara
Name: retinoic acid receptor, alpha
Synonyms: RARalpha1, RAR alpha 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19401
HGNC: HGNC:9864
Homologene: 20262
Cc2d2a
Name: coiled-coil and C2 domain containing 2A
Synonyms: 5730509K17Rik, b2b1035Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231214
Homologene: 18159
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 97,118,605 bp
  • T to C, chromosome 2 at 52,207,725 bp
  • A to G, chromosome 2 at 82,990,086 bp
  • T to C, chromosome 2 at 89,193,562 bp
  • G to A, chromosome 2 at 121,549,218 bp
  • G to A, chromosome 3 at 98,081,998 bp
  • A to G, chromosome 3 at 119,265,957 bp
  • G to A, chromosome 4 at 113,942,609 bp
  • T to C, chromosome 4 at 128,521,950 bp
  • A to G, chromosome 5 at 43,704,074 bp
  • A to T, chromosome 5 at 122,611,006 bp
  • C to T, chromosome 6 at 18,848,740 bp
  • A to T, chromosome 7 at 34,154,560 bp
  • G to A, chromosome 7 at 105,384,991 bp
  • A to T, chromosome 7 at 120,135,542 bp
  • G to T, chromosome 8 at 11,207,409 bp
  • A to G, chromosome 8 at 107,370,588 bp
  • T to A, chromosome 9 at 45,001,128 bp
  • T to C, chromosome 9 at 54,375,536 bp
  • A to T, chromosome 9 at 90,180,456 bp
  • T to C, chromosome 9 at 106,552,884 bp
  • A to T, chromosome 9 at 109,116,637 bp
  • A to G, chromosome 10 at 51,485,729 bp
  • T to A, chromosome 10 at 63,054,328 bp
  • T to C, chromosome 10 at 70,448,816 bp
  • T to C, chromosome 11 at 98,961,013 bp
  • T to C, chromosome 11 at 101,878,656 bp
  • T to C, chromosome 11 at 120,894,712 bp
  • A to G, chromosome 13 at 64,182,186 bp
  • A to G, chromosome 13 at 67,671,060 bp
  • A to G, chromosome 13 at 95,729,477 bp
  • A to T, chromosome 13 at 100,423,219 bp
  • T to A, chromosome 14 at 26,422,411 bp
  • T to A, chromosome 14 at 62,481,149 bp
  • C to A, chromosome 15 at 77,489,041 bp
  • T to A, chromosome 15 at 97,712,131 bp
  • A to T, chromosome 16 at 17,650,025 bp
  • A to T, chromosome 16 at 29,471,901 bp
  • A to T, chromosome 17 at 18,597,531 bp
  • T to C, chromosome 17 at 24,265,918 bp
  • A to T, chromosome 17 at 37,009,824 bp
  • T to A, chromosome 17 at 43,594,797 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6404 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044549-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.