Strain Name:
C57BL/6J-MtgxR6449Btlr/Mmmh
Stock Number:
044585-MU
Citation ID:
RRID:MMRRC_044585-MU
Other Names:
R6449 (G1)
Major Collection:

Strain Information

Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 8030492O04Rik, C230047C07Rik, AIP4, 6720481N21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16396
Homologene: 88442
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 2810430P21Rik, A230094E16Rik, Neurabin I, 4930518N04Rik, neurabin-I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Mkks
Name: McKusick-Kaufman syndrome
Synonyms: 1300013E18Rik, Bbs6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 59030
HGNC: HGNC:7108
Homologene: 10318
Psmd3
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Synonyms: Tstap91a, AntP91a, Psd3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22123
HGNC: HGNC:9560
Homologene: 2102
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Tcf12
Name: transcription factor 12
Synonyms: bHLHb20, HEB, HTF4, ALF1, ME1, HEBAlt, HTF-4, REB
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21406
Homologene: 40774
2610021A01Rik
Name: RIKEN cDNA 2610021A01 gene
Type: Gene
Species: Mouse
Chromosome: 7
Trim24
Name: tripartite motif-containing 24
Synonyms: Tif1a, A130082H20Rik, D430004I05Rik, TIF1alpha
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21848
Homologene: 20830
Gtpbp4
Name: GTP binding protein 4
Synonyms: 2610028C09Rik, Crfg, Nog1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 69237
VEGA: 13
Homologene: 7100
Kank2
Name: KN motif and ankyrin repeat domains 2
Synonyms: Ankrd25
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235041
VEGA: 9
Homologene: 9163
Rnf123
Name: ring finger protein 123
Synonyms: KPC1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 84585
Homologene: 11112
Map3k20
Name: mitogen-activated protein kinase kinase kinase 20
Synonyms: Zak, MLTKalpha, MLTKbeta, B230120H23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 65964
Homologene: 32331
Ano10
Name: anoctamin 10
Synonyms: Tmem16k
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102566
VEGA: 9
Homologene: 14314
Bmpr2
Name: bone morphogenetic protein receptor type 2
Synonyms: BMPRII, BMPR-II, 2610024H22Rik, BMP-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12168
HGNC: HGNC:1078
Homologene: 929
Cdhr1
Name: cadherin-related family member 1
Synonyms: Prcad, Pcdh21
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 170677
VEGA: 14
Homologene: 13215
Il2ra
Name: interleukin 2 receptor, alpha chain
Synonyms: Il2r, Ly-43, IL-2R alpha chain, CD25
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16184
HGNC: HGNC:6008
Homologene: 360
Kirrel3
Name: kirre like nephrin family adhesion molecule 3
Synonyms: 2900036G11Rik, 1500010O20Rik, Neph2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 67703
Homologene: 57050
Ccdc47
Name: coiled-coil domain containing 47
Synonyms: calumin, asp4, 2610204L23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67163
Homologene: 41351
Csnk1g2
Name: casein kinase 1, gamma 2
Synonyms: 2810429I12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103236
VEGA: 10
HGNC: HGNC:2455
Homologene: 100845
Raver2
Name: ribonucleoprotein, PTB-binding 2
Synonyms: A430091O22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242570
Homologene: 18829
Ttll5
Name: tubulin tyrosine ligase-like family, member 5
Synonyms: 1700048H13Rik, 4930556H18Rik, STAMP, 2310009M18Rik, D630041K24Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320244
Homologene: 9013
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: C130041O22Rik, Zfh-4, Zfh4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80892
Homologene: 23477
Ptprq
Name: protein tyrosine phosphatase receptor type Q
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237523
HGNC: HGNC:9679
Homologene: 83557
Fhod1
Name: formin homology 2 domain containing 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234686
Homologene: 136406
Plekhb2
Name: pleckstrin homology domain containing, family B (evectins) member 2
Synonyms: Phdc, evt-2, 2310009M15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226971
Homologene: 9938
Vmn2r70
Name: vomeronasal 2, receptor 70
Synonyms: EG620835
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 670940
Homologene: 115466
Sema4f
Name: sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain
Synonyms: Sema W
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20355
Homologene: 3147
Dsg1b
Name: desmoglein 1 beta
Synonyms: Dsg5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225256
HGNC: HGNC:3048
Homologene: 1463
Aadacl2fm2
Name: AADACL2 family member 2
Synonyms: Gm5538
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 433597
Homologene: 79018
Ubtfl1
Name: upstream binding transcription factor, RNA polymerase I-like 1
Synonyms: Hmgpi, B020006M18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 546118
Homologene: 110115
Col16a1
Name: collagen, type XVI, alpha 1
Synonyms: 2700007F12Rik, A530052M23Rik, [a]1 (XVI) collagen
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107581
HGNC: HGNC:2193
Homologene: 1397
Adam28
Name: a disintegrin and metallopeptidase domain 28
Synonyms: MDC-L, D430033C21Rik, C130072N01Rik, Dtgn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13522
VEGA: 14
HGNC: HGNC:206
Homologene: 40705
Or5w11
Name: olfactory receptor family 5 subfamily W member 11
Synonyms: GA_x6K02T2Q125-49133664-49134593, MOR177-4, Olfr1131
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258652
Homologene: 133019
Klra9
Name: killer cell lectin-like receptor subfamily A, member 9
Synonyms: Ly49I, LY49I1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16640
Homologene: 110821
Zfp639
Name: zinc finger protein 639
Synonyms: 6230400O18Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67778
Homologene: 9473
Cyp2c29
Name: cytochrome P450, family 2, subfamily c, polypeptide 29
Synonyms: Ahh-1, AHOHase, Ah-2, Cyp2c, AHOH, P450-2C
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13095
Homologene: 117948
Jazf1
Name: JAZF zinc finger 1
Synonyms: Tip27, Jaz1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 231986
Homologene: 18291
Or5k8
Name: olfactory receptor family 5 subfamily K member 8
Synonyms: MOR184-1, Olfr175-ps1, Olfr174, GA_x54KRFPKG5P-54993816-54992890, GA_x54KRFPKG5P-55026345-55025418, Olfr175, MOR184-10P
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 259004
Homologene: 17461
Gm5592
Name: predicted gene 5592
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434172
Homologene: 45597
Pacsin3
Name: protein kinase C and casein kinase substrate in neurons 3
Synonyms: 4921507A02Rik, 6330413E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 80708
HGNC: HGNC:8572
Homologene: 41117
Krtap27-1
Name: keratin associated protein 27-1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239933
Homologene: 90787
Ntaq1
Name: N-terminal glutamine amidase 1
Synonyms: 2410187C16Rik, Wdyhv1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76773
VEGA: 15
Homologene: 9968
Or5c1
Name: olfactory receptor family 5 subfamily C member 1
Synonyms: Olfr368, GA_x6K02T2NLDC-34015743-34016726, MOR178-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258371
HGNC: HGNC:8331
Homologene: 71970
Tcl1b2
Name: T cell leukemia/lymphoma 1B, 2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 27381
Homologene: 83274
Dnase1l1
Name: deoxyribonuclease 1-like 1
Synonyms: G4.8, Dnl1ll, 2310005K03Rik, Dnase1ll
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 69537
HGNC: HGNC:2957
Homologene: 4896
Pdcd7
Name: programmed cell death 7
Synonyms: ES18
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 50996
VEGA: 9
HGNC: HGNC:8767
Homologene: 4170
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,864,483 bp
  • T to A, chromosome 1 at 59,867,437 bp
  • A to G, chromosome 2 at 11,680,362 bp
  • A to G, chromosome 2 at 37,331,825 bp
  • T to C, chromosome 2 at 72,398,414 bp
  • T to C, chromosome 2 at 87,629,149 bp
  • A to T, chromosome 2 at 91,260,169 bp
  • C to A, chromosome 2 at 136,874,286 bp
  • T to C, chromosome 2 at 155,163,395 bp
  • A to G, chromosome 3 at 5,242,428 bp
  • A to G, chromosome 3 at 32,519,661 bp
  • A to T, chromosome 3 at 59,745,551 bp
  • A to G, chromosome 4 at 101,133,672 bp
  • G to A, chromosome 4 at 130,066,693 bp
  • A to G, chromosome 6 at 5,057,458 bp
  • G to A, chromosome 6 at 37,903,652 bp
  • A to C, chromosome 6 at 52,777,655 bp
  • A to G, chromosome 6 at 82,917,870 bp
  • G to T, chromosome 6 at 130,179,032 bp
  • G to A, chromosome 7 at 41,288,586 bp
  • C to T, chromosome 7 at 41,625,874 bp
  • A to T, chromosome 7 at 85,564,949 bp
  • T to A, chromosome 8 at 105,330,237 bp
  • T to A, chromosome 9 at 18,409,629 bp
  • G to T, chromosome 9 at 21,780,562 bp
  • A to G, chromosome 9 at 34,990,973 bp
  • A to G, chromosome 9 at 65,356,775 bp
  • A to T, chromosome 9 at 71,868,268 bp
  • G to A, chromosome 9 at 108,056,053 bp
  • T to C, chromosome 9 at 122,201,688 bp
  • A to G, chromosome 10 at 80,640,072 bp
  • C to T, chromosome 10 at 107,705,583 bp
  • A to G, chromosome 11 at 98,685,640 bp
  • A to G, chromosome 11 at 106,204,985 bp
  • T to C, chromosome 12 at 86,024,276 bp
  • G to T, chromosome 12 at 105,153,002 bp
  • A to T, chromosome 13 at 8,990,737 bp
  • T to C, chromosome 14 at 37,090,597 bp
  • T to C, chromosome 14 at 68,630,667 bp
  • T to G, chromosome 15 at 58,150,598 bp
  • A to T, chromosome 16 at 58,824,526 bp
  • C to T, chromosome 16 at 88,671,053 bp
  • A to C, chromosome 17 at 84,429,173 bp
  • A to T, chromosome 18 at 20,394,441 bp
  • G to T, chromosome 19 at 39,290,867 bp
  • C to T, chromosome X at 74,277,038 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6449 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044585-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.