Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6452Btlr/Mmmh
Stock Number:
044588-MU
Citation ID:
RRID:MMRRC_044588-MU
Other Names:
R6452 (G1)
Major Collection:

Strain Information

Dhx35
Name: DEAH-box helicase 35
Synonyms: Ddx35, 1200009D07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71715
Homologene: 6406
Larp4b
Name: La ribonucleoprotein 4B
Synonyms: D13Wsu64e, Larp5, A130023E24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 217980
Homologene: 18195
Ranbp2
Name: RAN binding protein 2
Synonyms: A430087B05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19386
VEGA: 10
HGNC: HGNC:9848
Homologene: 87808
Fasn
Name: fatty acid synthase
Synonyms: FAS, A630082H08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14104
HGNC: HGNC:3594
Homologene: 55800
Cetn3
Name: centrin 3
Synonyms: MmCEN3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12626
VEGA: 13
HGNC: HGNC:1868
Homologene: 74521
Zfp442
Name: zinc finger protein 442
Synonyms: OTTMUSG00000015730
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 668923
Homologene: 136215
Itgb3
Name: integrin beta 3
Synonyms: platelet glycoprotein IIIa (GP3A), CD61
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16416
HGNC: HGNC:6156
Homologene: 55444
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 98,421,212 bp
  • A to G, chromosome 1 at 160,453,761 bp
  • A to T, chromosome 2 at 52,179,483 bp
  • G to A, chromosome 2 at 150,408,108 bp
  • A to T, chromosome 2 at 155,815,753 bp
  • A to G, chromosome 2 at 158,831,687 bp
  • G to T, chromosome 2 at 160,965,498 bp
  • C to G, chromosome 2 at 170,119,294 bp
  • C to A, chromosome 2 at 173,527,907 bp
  • T to C, chromosome 3 at 30,655,733 bp
  • A to G, chromosome 3 at 122,544,549 bp
  • A to G, chromosome 4 at 83,864,290 bp
  • T to C, chromosome 4 at 101,941,443 bp
  • T to C, chromosome 4 at 154,023,614 bp
  • A to T, chromosome 5 at 8,579,175 bp
  • A to G, chromosome 5 at 89,228,980 bp
  • A to C, chromosome 5 at 142,727,012 bp
  • G to A, chromosome 6 at 82,917,662 bp
  • G to T, chromosome 6 at 130,065,366 bp
  • G to A, chromosome 6 at 146,823,207 bp
  • C to T, chromosome 7 at 11,162,072 bp
  • A to G, chromosome 7 at 62,380,384 bp
  • T to A, chromosome 7 at 97,347,662 bp
  • C to T, chromosome 7 at 98,073,167 bp
  • A to G, chromosome 7 at 108,286,893 bp
  • A to G, chromosome 8 at 33,572,816 bp
  • A to G, chromosome 8 at 123,079,423 bp
  • T to C, chromosome 9 at 51,146,190 bp
  • G to T, chromosome 10 at 58,478,157 bp
  • A to G, chromosome 10 at 128,807,490 bp
  • A to T, chromosome 11 at 59,752,783 bp
  • A to T, chromosome 11 at 67,292,449 bp
  • A to T, chromosome 11 at 67,305,739 bp
  • A to T, chromosome 11 at 70,320,005 bp
  • G to T, chromosome 11 at 76,207,146 bp
  • T to A, chromosome 11 at 87,732,253 bp
  • A to G, chromosome 11 at 88,081,670 bp
  • T to G, chromosome 11 at 102,604,985 bp
  • A to T, chromosome 11 at 104,188,191 bp
  • T to A, chromosome 11 at 104,633,464 bp
  • G to A, chromosome 11 at 116,455,888 bp
  • G to T, chromosome 11 at 120,815,411 bp
  • T to C, chromosome 12 at 100,835,209 bp
  • T to C, chromosome 13 at 9,147,467 bp
  • T to A, chromosome 13 at 22,827,670 bp
  • T to C, chromosome 13 at 27,352,797 bp
  • C to T, chromosome 13 at 81,784,678 bp
  • A to G, chromosome 13 at 119,488,115 bp
  • A to T, chromosome 15 at 75,911,438 bp
  • A to T, chromosome 15 at 82,616,089 bp
  • T to G, chromosome 15 at 84,204,097 bp
  • C to A, chromosome 16 at 57,506,800 bp
  • A to C, chromosome 17 at 18,583,855 bp
  • A to C, chromosome 17 at 21,509,613 bp
  • T to C, chromosome 17 at 33,215,945 bp
  • G to A, chromosome 17 at 44,085,264 bp
  • G to A, chromosome 18 at 9,280,457 bp
  • A to G, chromosome 18 at 24,695,941 bp
  • A to G, chromosome 18 at 36,531,459 bp
  • A to T, chromosome 19 at 7,014,737 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6452 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044588-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.