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Strain Name:
C57BL/6J-MtgxR6453Btlr/Mmmh
Stock Number:
044589-MU
Citation ID:
RRID:MMRRC_044589-MU
Other Names:
R6453 (G1)
Major Collection:

Strain Information

Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Gldc
Name: glycine decarboxylase
Synonyms: D19Wsu57e, D030049L12Rik, b2b2679Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 104174
VEGA: 19
HGNC: HGNC:4313
Homologene: 141
Pik3cd
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: p110delta, 2410099E07Rik, 2610208K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18707
HGNC: HGNC:8977
Homologene: 3686
Gpatch4
Name: G patch domain containing 4
Synonyms: 2610029K21Rik, Gpatc4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66614
Homologene: 9203
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Rnf169
Name: ring finger protein 169
Synonyms: 2900057K09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108937
Homologene: 47510
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 11,798,558 bp
  • A to T, chromosome 1 at 40,742,621 bp
  • G to A, chromosome 1 at 45,339,378 bp
  • A to T, chromosome 1 at 54,657,704 bp
  • A to G, chromosome 1 at 75,417,972 bp
  • A to G, chromosome 1 at 136,482,304 bp
  • A to G, chromosome 1 at 139,143,948 bp
  • C to T, chromosome 2 at 69,047,169 bp
  • A to G, chromosome 2 at 142,176,625 bp
  • G to T, chromosome 2 at 144,549,134 bp
  • C to T, chromosome 2 at 165,411,947 bp
  • A to G, chromosome 2 at 173,528,259 bp
  • A to G, chromosome 3 at 80,740,974 bp
  • A to G, chromosome 3 at 88,055,005 bp
  • A to G, chromosome 3 at 95,578,257 bp
  • G to T, chromosome 4 at 82,914,825 bp
  • A to G, chromosome 4 at 149,652,302 bp
  • T to A, chromosome 5 at 14,676,789 bp
  • G to A, chromosome 5 at 121,350,592 bp
  • A to G, chromosome 5 at 142,096,921 bp
  • T to C, chromosome 5 at 147,683,941 bp
  • A to G, chromosome 5 at 147,886,390 bp
  • G to A, chromosome 6 at 65,453,030 bp
  • A to G, chromosome 6 at 112,315,828 bp
  • C to G, chromosome 7 at 3,737,320 bp
  • C to T, chromosome 7 at 19,141,297 bp
  • A to T, chromosome 7 at 55,935,821 bp
  • CGGCGGCGG to CGGCGGCGGGGGCGGCGG, chromosome 7 at 97,579,917 bp
  • C to T, chromosome 7 at 98,073,167 bp
  • C to T, chromosome 7 at 99,935,227 bp
  • T to A, chromosome 7 at 139,875,427 bp
  • A to G, chromosome 8 at 123,079,423 bp
  • T to C, chromosome 9 at 38,473,575 bp
  • A to T, chromosome 10 at 41,550,825 bp
  • A to G, chromosome 10 at 128,807,490 bp
  • A to G, chromosome 11 at 55,282,216 bp
  • A to G, chromosome 11 at 68,973,552 bp
  • T to A, chromosome 11 at 69,350,112 bp
  • T to G, chromosome 11 at 72,782,760 bp
  • G to A, chromosome 11 at 96,158,727 bp
  • T to C, chromosome 11 at 116,293,969 bp
  • T to C, chromosome 12 at 8,534,490 bp
  • C to A, chromosome 12 at 36,314,408 bp
  • A to G, chromosome 12 at 55,738,319 bp
  • A to T, chromosome 13 at 100,814,689 bp
  • T to A, chromosome 15 at 81,011,314 bp
  • A to T, chromosome 15 at 84,328,925 bp
  • A to T, chromosome 16 at 15,630,409 bp
  • C to T, chromosome 17 at 35,344,895 bp
  • C to T, chromosome 17 at 35,568,212 bp
  • T to A, chromosome 17 at 48,316,736 bp
  • T to C, chromosome 17 at 84,416,323 bp
  • T to A, chromosome 17 at 87,985,739 bp
  • T to A, chromosome 18 at 21,148,739 bp
  • A to T, chromosome 19 at 4,744,180 bp
  • G to A, chromosome 19 at 18,829,990 bp
  • A to T, chromosome 19 at 30,116,517 bp
  • G to A, chromosome 19 at 34,246,657 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6453 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044589-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.