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Strain Name:
C57BL/6J-MtgxR6459Btlr/Mmmh
Stock Number:
044594-MU
Citation ID:
RRID:MMRRC_044594-MU
Other Names:
R6459 (G1)
Major Collection:

Strain Information

Nefh
Name: neurofilament, heavy polypeptide
Synonyms: NF-H, NF200, NEFH
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380684
HGNC: HGNC:7737
Homologene: 40755
Pak1
Name: p21 (RAC1) activated kinase 1
Synonyms: PAK-1, Paka
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18479
HGNC: HGNC:8590
Homologene: 1936
Atl3
Name: atlastin GTPase 3
Synonyms: 4633402C03Rik, 5730596K20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 109168
VEGA: 19
Homologene: 9149
Epop
Name: elongin BC and polycomb repressive complex 2 associated protein
Synonyms: E13, E130012A19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103551
Homologene: 82351
Lrrc41
Name: leucine rich repeat containing 41
Synonyms: D730026A16Rik, MUF1, D630045E04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230654
Homologene: 4645
Pcm1
Name: pericentriolar material 1
Synonyms: 9430077F19Rik, 2600002H09Rik, C030044G17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
Specc1l
Name: sperm antigen with calponin homology and coiled-coil domains 1-like
Synonyms: 4930470P14Rik, 9530057A13Rik, 4932439K10Rik, Specc1l, Cytsa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74392
VEGA: 10
Homologene: 15610
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 43,123,653 bp
  • T to A, chromosome 1 at 79,436,290 bp
  • T to C, chromosome 1 at 150,454,301 bp
  • C to A, chromosome 2 at 26,423,500 bp
  • G to T, chromosome 2 at 85,951,518 bp
  • T to A, chromosome 2 at 86,483,229 bp
  • A to G, chromosome 2 at 150,667,920 bp
  • G to A, chromosome 2 at 168,668,013 bp
  • A to G, chromosome 3 at 8,899,980 bp
  • C to T, chromosome 3 at 53,478,329 bp
  • T to C, chromosome 3 at 79,481,634 bp
  • T to C, chromosome 4 at 116,088,780 bp
  • C to T, chromosome 4 at 124,856,842 bp
  • T to C, chromosome 4 at 135,557,433 bp
  • T to C, chromosome 5 at 134,914,915 bp
  • T to C, chromosome 6 at 18,258,236 bp
  • A to T, chromosome 6 at 68,256,741 bp
  • A to G, chromosome 6 at 97,487,640 bp
  • T to C, chromosome 7 at 29,015,654 bp
  • A to G, chromosome 7 at 30,712,462 bp
  • A to G, chromosome 7 at 55,979,606 bp
  • A to T, chromosome 7 at 83,722,321 bp
  • C to A, chromosome 7 at 83,722,328 bp
  • A to G, chromosome 7 at 97,907,881 bp
  • T to C, chromosome 7 at 107,753,255 bp
  • A to C, chromosome 8 at 5,098,581 bp
  • G to A, chromosome 8 at 41,261,036 bp
  • A to G, chromosome 8 at 125,444,484 bp
  • A to G, chromosome 9 at 27,051,755 bp
  • A to G, chromosome 9 at 38,930,665 bp
  • T to C, chromosome 10 at 7,126,820 bp
  • C to T, chromosome 10 at 62,937,477 bp
  • C to T, chromosome 10 at 69,991,747 bp
  • T to C, chromosome 10 at 75,246,167 bp
  • T to A, chromosome 10 at 102,385,127 bp
  • T to C, chromosome 11 at 4,939,551 bp
  • C to A, chromosome 11 at 76,424,989 bp
  • T to C, chromosome 11 at 97,628,507 bp
  • T to C, chromosome 11 at 115,821,680 bp
  • A to G, chromosome 13 at 20,999,942 bp
  • T to C, chromosome 13 at 106,865,769 bp
  • A to G, chromosome 15 at 102,518,479 bp
  • A to G, chromosome 16 at 11,153,161 bp
  • A to G, chromosome 16 at 20,594,679 bp
  • A to G, chromosome 16 at 23,012,115 bp
  • C to T, chromosome 16 at 59,216,020 bp
  • T to A, chromosome 17 at 22,200,546 bp
  • T to C, chromosome 17 at 50,047,306 bp
  • T to C, chromosome 17 at 88,636,468 bp
  • C to T, chromosome 18 at 20,836,868 bp
  • A to T, chromosome 19 at 6,854,878 bp
  • A to T, chromosome 19 at 7,520,798 bp
  • G to T, chromosome 19 at 12,901,005 bp
  • A to T, chromosome 19 at 16,664,018 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6459 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044594-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.