Strain Name:
C57BL/6J-MtgxR6464Btlr/Mmmh
Stock Number:
044597-MU
Citation ID:
RRID:MMRRC_044597-MU
Other Names:
R6464 (G1)
Major Collection:

Strain Information

Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: Gukmi1, Baiap1, BAP1, WWP3, AIP3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Ptdss2
Name: phosphatidylserine synthase 2
Synonyms: PSS2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 27388
Homologene: 8462
Rpl23
Name: ribosomal protein L23
Synonyms: 2810009A01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 65019
Homologene: 68103
Tesk2
Name: testis-specific kinase 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230661
Homologene: 5188
Znfx1
Name: zinc finger, NFX1-type containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 98999
Homologene: 10877
Kif20b
Name: kinesin family member 20B
Synonyms: 33cex, N-6 kinesin, C330014J10Rik, Kif20b, Mphosph1, magoo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240641
VEGA: 19
HGNC: HGNC:7212
Homologene: 9418
Clasp2
Name: CLIP associating protein 2
Synonyms: 8030404L10Rik, CLASP2gamma, CLASP2beta, CLASP2, 1500004F14Rik, CLASP2alpha
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76499
VEGA: 9
Homologene: 24944
Ube3a
Name: ubiquitin protein ligase E3A
Synonyms: Hpve6a, A130086L21Rik, 5830462N02Rik, E6-AP ubiquitin protein ligase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22215
Homologene: 7988
Naa25
Name: N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Synonyms: 4833422K13Rik, C330023M02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231713
Homologene: 6891
Fkbp15
Name: FK506 binding protein 15
Synonyms: FKBP133, C430014M02Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 338355
Homologene: 28743
Lpar1
Name: lysophosphatidic acid receptor 1
Synonyms: Edg2, Gpcr26, LPA1, Kdt2, vzg-1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14745
HGNC: HGNC:3166
Homologene: 1072
Apoe
Name: apolipoprotein E
Synonyms: Apoe
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11816
HGNC: HGNC:613
Homologene: 30951
Slc39a14
Name: solute carrier family 39 (zinc transporter), member 14
Synonyms: G630015O18Rik, Zip14
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213053
Homologene: 15037
Kif7
Name: kinesin family member 7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16576
Homologene: 72555
Fam13b
Name: family with sequence similarity 13, member B
Synonyms: 2610024E20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225358
VEGA: 18
HGNC: HGNC:1335
Homologene: 9585
Dsg3
Name: desmoglein 3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13512
VEGA: 18
HGNC: HGNC:3050
Homologene: 55513
Tnik
Name: TRAF2 and NCK interacting kinase
Synonyms: 4831440I19Rik, C530008O15Rik, 1500031A17Rik, C630040K21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 665113
Homologene: 77943
Cfap70
Name: cilia and flagella associated protein 70
Synonyms: 5330402L21Rik, Ttc18
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 76670
Homologene: 17048
Helz2
Name: helicase with zinc finger 2, transcriptional coactivator
Synonyms: BC006779
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 229003
Homologene: 14118
Vinac1
Name: vinculin/alpha-catenin family member 1
Synonyms: Gm14025
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 668894
Homologene: 86340
Vwf
Name: Von Willebrand factor
Synonyms: B130011O06Rik, 6820430P06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22371
Homologene: 466
Scn5a
Name: sodium channel, voltage-gated, type V, alpha
Synonyms: Nav1.5, mH1, SkM2, Nav1.5c
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20271
Homologene: 22738
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Vmn2r1
Name: vomeronasal 2, receptor 1
Synonyms: EG56544, V2r83
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56544
Homologene: 84832
Helt
Name: helt bHLH transcription factor
Synonyms: megane, Heslike
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234219
Homologene: 18357
Col6a6
Name: collagen, type VI, alpha 6
Synonyms: E330026B02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245026
Homologene: 18260
Flg
Name: filaggrin
Synonyms: ft, fillagrin, profilaggrin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14246
VEGA: 3
Cdkl4
Name: cyclin dependent kinase like 4
Synonyms: LOC381113
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381113
VEGA: 17
Homologene: 28725
Otoa
Name: otoancorin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246190
Homologene: 71803
Arhgef10l
Name: Rho guanine nucleotide exchange factor 10-like
Synonyms: 2810441C07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72754
Homologene: 10017
Myo1f
Name: myosin IF
Synonyms: C330006B10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17916
HGNC: HGNC:7600
Homologene: 56276
Pde4dip
Name: phosphodiesterase 4D interacting protein (myomegalin)
Synonyms: D3Bwg1078e, D130016K21Rik, 9430063L05Rik, Usmg4, 4732458A06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 83679
Homologene: 66961
Cyp27a1
Name: cytochrome P450, family 27, subfamily a, polypeptide 1
Synonyms: 1300013A03Rik, Cyp27, cholesterol 27 hydroxylase
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 104086
HGNC: HGNC:2605
Homologene: 36040
Ugt1a1
Name: UDP glucuronosyltransferase 1 family, polypeptide A1
Synonyms: UgtBr1, UGT1A01, Udpgt-1a, Gnt1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 394436
Homologene: 128034
Cntnap5a
Name: contactin associated protein-like 5A
Synonyms: Caspr5-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 636808
Homologene: 43974
Abcc1
Name: ATP-binding cassette, sub-family C member 1
Synonyms: Mrp1, Abcc1a, Mdrap, MRP, Abcc1b
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17250
HGNC: HGNC:51
Homologene: 133779
Clip2
Name: CAP-GLY domain containing linker protein 2
Synonyms: CLIP-115, WSCR4, Cyln2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269713
HGNC: HGNC:2586
Homologene: 20718
Vmn1r74
Name: vomeronasal 1 receptor 74
Synonyms: V1rg5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 171240
Homologene: 110796
Klra9
Name: killer cell lectin-like receptor subfamily A, member 9
Synonyms: LY49I1, Ly49I
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16640
Homologene: 110821
Cyp2c29
Name: cytochrome P450, family 2, subfamily c, polypeptide 29
Synonyms: AHOHase, AHOH, Ahh-1, Cyp2c, P450-2C, Ah-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 13095
Homologene: 117948
Ceacam5
Name: CEA cell adhesion molecule 5
Synonyms: 1600029H12Rik, Psg30
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73250
HGNC: HGNC:1819
Homologene: 115938
Meltf
Name: melanotransferrin
Synonyms: MTf, melanotransferrin, CD228, Mfi2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 30060
VEGA: 16
HGNC: HGNC:7037
Homologene: 4335
Lipf
Name: lipase, gastric
Synonyms: 2310051B21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67717
HGNC: HGNC:6622
Homologene: 68139
Zfp703
Name: zinc finger protein 703
Synonyms: End2, 1110032O19Rik, Zeppo1, Csmn1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 353310
Homologene: 81391
Fam131c
Name: family with sequence similarity 131, member C
Synonyms: LOC277743
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 277743
Homologene: 18806
Krtap9-20
Name: keratin associated protein 9-20
Synonyms: Gm11559
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100415785
Homologene: 136354
Rdh8
Name: retinol dehydrogenase 8
Synonyms: LOC235033, prRDH
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235033
VEGA: 9
Homologene: 41062
Or8u3-ps
Name: olfactory receptor family 8 subfamily U member 3
Synonyms: Olfr1038-ps, GA_x6K02T2Q125-47591072-47592031, MOR185-3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259015
Homologene: 17463
Smok2a
Name: sperm motility kinase 2A
Synonyms: Smok2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27263
Homologene: 128738
Wdr97
Name: WD repeat domain 97
Synonyms: Gm35339
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 102638882
Homologene: 131539
Or10ag55-ps1
Name: olfactory receptor family 10 subfamily AG member 55, pseudogene 1
Synonyms: Olfr1117-ps1, MOR264-27_p, GA_x6K02T2Q125-48769330-48770299, MOR264-10P
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258110
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,735,888 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • G to C, chromosome 1 at 88,257,802 bp
  • A to G, chromosome 1 at 116,184,408 bp
  • A to T, chromosome 2 at 86,122,408 bp
  • A to T, chromosome 2 at 87,309,607 bp
  • T to C, chromosome 2 at 129,039,545 bp
  • T to C, chromosome 2 at 167,046,922 bp
  • T to C, chromosome 2 at 181,235,069 bp
  • G to A, chromosome 3 at 28,611,970 bp
  • G to A, chromosome 3 at 64,101,345 bp
  • T to A, chromosome 3 at 93,281,381 bp
  • C to T, chromosome 3 at 97,710,344 bp
  • A to G, chromosome 4 at 58,486,875 bp
  • T to C, chromosome 4 at 62,308,078 bp
  • T to C, chromosome 4 at 88,592,250 bp
  • A to C, chromosome 4 at 116,802,849 bp
  • A to C, chromosome 4 at 140,586,815 bp
  • T to C, chromosome 4 at 141,382,342 bp
  • G to A, chromosome 5 at 121,417,961 bp
  • T to C, chromosome 5 at 134,491,925 bp
  • A to T, chromosome 6 at 93,699,789 bp
  • T to C, chromosome 6 at 125,639,400 bp
  • G to T, chromosome 6 at 130,179,032 bp
  • A to T, chromosome 7 at 11,847,204 bp
  • T to C, chromosome 7 at 17,747,466 bp
  • G to A, chromosome 7 at 19,697,536 bp
  • T to A, chromosome 7 at 59,276,183 bp
  • A to G, chromosome 7 at 79,714,094 bp
  • A to G, chromosome 7 at 121,102,605 bp
  • T to C, chromosome 7 at 141,152,211 bp
  • C to G, chromosome 8 at 26,979,327 bp
  • T to C, chromosome 8 at 46,292,534 bp
  • G to T, chromosome 9 at 20,823,400 bp
  • C to T, chromosome 9 at 105,788,953 bp
  • A to G, chromosome 9 at 113,773,717 bp
  • T to C, chromosome 9 at 119,534,580 bp
  • T to C, chromosome 11 at 97,778,285 bp
  • T to C, chromosome 11 at 99,864,716 bp
  • T to C, chromosome 14 at 20,401,039 bp
  • G to C, chromosome 14 at 70,306,728 bp
  • A to G, chromosome 15 at 76,362,777 bp
  • T to A, chromosome 16 at 14,447,490 bp
  • T to C, chromosome 16 at 31,890,776 bp
  • C to T, chromosome 17 at 13,226,430 bp
  • T to C, chromosome 17 at 33,576,647 bp
  • A to T, chromosome 17 at 80,525,352 bp
  • A to C, chromosome 18 at 20,533,526 bp
  • A to T, chromosome 18 at 34,473,631 bp
  • A to T, chromosome 19 at 33,973,544 bp
  • G to A, chromosome 19 at 34,934,441 bp
  • A to G, chromosome 19 at 39,329,225 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6464 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044597-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.