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Strain Name:
C57BL/6J-MtgxR6489Btlr/Mmmh
Stock Number:
044621-MU
Citation ID:
RRID:MMRRC_044621-MU
Other Names:
R6489 (G1)
Major Collection:

Strain Information

App
Name: amyloid beta precursor protein
Synonyms: betaAPP, Abeta, protease nexin II, Adap, Cvap, appican, E030013M08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11820
VEGA: 16
HGNC: HGNC:620
Homologene: 56379
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68926
Homologene: 73649
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Vsnl1
Name: visinin-like 1
Synonyms: VILIP
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 26950
VEGA: 12
Homologene: 2542
Nup93
Name: nucleoporin 93
Synonyms: 2410008G02Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 71805
Homologene: 40971
Dhx9
Name: DExH-box helicase 9
Synonyms: leukophysin, nuclear DNA helicase II, RNA helicase, Ddx9, NDH II, NDHII, RHA
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13211
HGNC: HGNC:2750
Homologene: 1039
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,330,942 bp
  • T to C, chromosome 1 at 63,307,478 bp
  • T to A, chromosome 1 at 74,946,511 bp
  • G to A, chromosome 1 at 130,717,538 bp
  • T to C, chromosome 1 at 153,456,643 bp
  • A to C, chromosome 2 at 33,411,546 bp
  • A to G, chromosome 2 at 76,814,718 bp
  • A to C, chromosome 2 at 111,814,060 bp
  • T to C, chromosome 2 at 129,269,114 bp
  • A to G, chromosome 3 at 59,257,407 bp
  • C to A, chromosome 3 at 88,643,014 bp
  • G to A, chromosome 3 at 97,755,591 bp
  • A to T, chromosome 3 at 152,279,008 bp
  • A to G, chromosome 4 at 33,162,855 bp
  • T to C, chromosome 4 at 41,203,574 bp
  • C to A, chromosome 4 at 58,100,066 bp
  • A to T, chromosome 4 at 141,632,033 bp
  • T to A, chromosome 5 at 4,757,336 bp
  • G to T, chromosome 5 at 25,264,966 bp
  • T to G, chromosome 5 at 120,120,130 bp
  • T to C, chromosome 6 at 3,376,896 bp
  • T to C, chromosome 7 at 81,624,278 bp
  • A to T, chromosome 7 at 99,690,555 bp
  • T to C, chromosome 7 at 103,740,668 bp
  • T to C, chromosome 7 at 134,990,541 bp
  • T to C, chromosome 8 at 84,031,550 bp
  • T to A, chromosome 8 at 94,302,088 bp
  • T to A, chromosome 8 at 94,335,004 bp
  • A to G, chromosome 8 at 106,163,194 bp
  • T to C, chromosome 8 at 107,050,301 bp
  • A to G, chromosome 9 at 26,826,831 bp
  • A to T, chromosome 9 at 57,613,726 bp
  • A to G, chromosome 9 at 72,015,636 bp
  • A to T, chromosome 10 at 34,092,506 bp
  • G to A, chromosome 10 at 69,991,629 bp
  • A to T, chromosome 11 at 61,749,064 bp
  • T to C, chromosome 11 at 70,010,319 bp
  • T to A, chromosome 11 at 73,603,439 bp
  • G to C, chromosome 11 at 106,349,180 bp
  • T to G, chromosome 12 at 11,332,218 bp
  • A to G, chromosome 12 at 111,428,697 bp
  • T to A, chromosome 13 at 11,834,007 bp
  • T to C, chromosome 13 at 48,826,182 bp
  • G to T, chromosome 13 at 98,250,609 bp
  • G to A, chromosome 14 at 7,867,551 bp
  • T to C, chromosome 14 at 47,549,023 bp
  • C to A, chromosome 14 at 55,660,391 bp
  • C to T, chromosome 14 at 60,300,514 bp
  • A to T, chromosome 14 at 108,911,303 bp
  • C to A, chromosome 15 at 54,462,213 bp
  • A to T, chromosome 15 at 98,257,007 bp
  • G to T, chromosome 16 at 85,056,520 bp
  • T to G, chromosome 16 at 91,655,156 bp
  • A to T, chromosome 16 at 93,630,398 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • T to A, chromosome 17 at 56,998,979 bp
  • T to A, chromosome 18 at 20,471,363 bp
  • C to T, chromosome 18 at 57,291,807 bp
  • T to C, chromosome 18 at 61,007,543 bp
  • T to C, chromosome 18 at 75,080,226 bp
  • C to T, chromosome 19 at 47,109,752 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6489 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044621-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.