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Strain Name:
C57BL/6J-MtgxR6492Btlr/Mmmh
Stock Number:
044624-MU
Citation ID:
RRID:MMRRC_044624-MU
Other Names:
R6492 (G1)
Major Collection:

Strain Information

Tyrp1
Name: tyrosinase-related protein 1
Synonyms: Tyrp, isa, TRP-1, TRP1, Oca3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22178
Homologene: 464
Mc3r
Name: melanocortin 3 receptor
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17201
HGNC: HGNC:6931
Homologene: 7412
Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra-5, Acra5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Prss12
Name: serine protease 12 neurotrypsin (motopsin)
Synonyms: Bssp-3, motopsin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 19142
HGNC: HGNC:9477
Homologene: 7490
Plcb4
Name: phospholipase C, beta 4
Synonyms: C230058B11Rik, A930039J07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Wwp1
Name: WW domain containing E3 ubiquitin protein ligase 1
Synonyms: SDRP1, Tiul1, AIP5, 8030445B08Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107568
Homologene: 21385
Sin3b
Name: transcriptional regulator, SIN3B (yeast)
Synonyms: 2810430C10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20467
Homologene: 81810
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 12,989,927 bp
  • G to C, chromosome 1 at 19,104,478 bp
  • T to C, chromosome 1 at 69,539,042 bp
  • T to G, chromosome 1 at 97,125,813 bp
  • A to G, chromosome 1 at 127,471,564 bp
  • T to C, chromosome 1 at 131,259,218 bp
  • A to T, chromosome 1 at 138,113,562 bp
  • T to C, chromosome 2 at 9,951,160 bp
  • T to A, chromosome 2 at 31,801,655 bp
  • T to C, chromosome 2 at 45,110,496 bp
  • T to A, chromosome 2 at 86,228,387 bp
  • T to C, chromosome 2 at 86,298,646 bp
  • C to T, chromosome 2 at 87,510,422 bp
  • C to G, chromosome 2 at 87,532,741 bp
  • C to T, chromosome 2 at 135,973,071 bp
  • G to A, chromosome 2 at 172,249,154 bp
  • T to C, chromosome 3 at 19,982,022 bp
  • A to G, chromosome 3 at 123,447,399 bp
  • T to A, chromosome 3 at 144,957,298 bp
  • C to T, chromosome 3 at 146,624,577 bp
  • G to A, chromosome 4 at 19,650,299 bp
  • A to G, chromosome 4 at 80,840,781 bp
  • G to T, chromosome 4 at 100,952,118 bp
  • G to A, chromosome 4 at 111,547,272 bp
  • A to T, chromosome 4 at 117,876,967 bp
  • A to G, chromosome 4 at 120,921,537 bp
  • G to A, chromosome 4 at 141,463,383 bp
  • A to G, chromosome 5 at 103,501,612 bp
  • C to T, chromosome 6 at 121,654,505 bp
  • T to C, chromosome 7 at 19,882,592 bp
  • T to C, chromosome 7 at 24,979,304 bp
  • T to C, chromosome 7 at 44,471,154 bp
  • T to C, chromosome 7 at 67,736,061 bp
  • A to G, chromosome 7 at 101,500,442 bp
  • C to A, chromosome 7 at 104,925,645 bp
  • A to G, chromosome 7 at 118,984,577 bp
  • G to T, chromosome 7 at 127,522,145 bp
  • T to A, chromosome 8 at 72,733,490 bp
  • A to G, chromosome 8 at 85,740,349 bp
  • T to A, chromosome 8 at 95,264,424 bp
  • A to G, chromosome 9 at 54,998,063 bp
  • T to C, chromosome 10 at 4,369,711 bp
  • C to T, chromosome 10 at 39,154,987 bp
  • T to C, chromosome 11 at 5,521,005 bp
  • A to T, chromosome 11 at 43,218,957 bp
  • C to T, chromosome 11 at 106,375,236 bp
  • A to T, chromosome 12 at 8,008,261 bp
  • T to C, chromosome 12 at 70,054,534 bp
  • A to T, chromosome 13 at 21,525,227 bp
  • A to T, chromosome 13 at 90,883,722 bp
  • A to G, chromosome 14 at 52,035,445 bp
  • A to G, chromosome 15 at 12,861,706 bp
  • T to C, chromosome 15 at 84,303,872 bp
  • T to A, chromosome 16 at 3,928,612 bp
  • T to A, chromosome 16 at 8,488,891 bp
  • G to T, chromosome 16 at 66,784,828 bp
  • A to T, chromosome 18 at 14,651,088 bp
  • C to T, chromosome 18 at 30,324,562 bp
  • C to A, chromosome 18 at 33,464,305 bp
  • A to G, chromosome 18 at 37,643,106 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6492 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044624-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.