Strain Name:
C57BL/6J-MtgxR6499Btlr/Mmmh
Stock Number:
044631-MU
Citation ID:
RRID:MMRRC_044631-MU
Other Names:
R6499 (G1)
Major Collection:

Strain Information

Angpt2
Name: angiopoietin 2
Synonyms: Ang2, Ang-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11601
HGNC: HGNC:485
Homologene: 22401
Ccna2
Name: cyclin A2
Synonyms: Ccn-1, Cyca, Ccna, CycA2, Ccn1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12428
HGNC: HGNC:1578
Homologene: 55562
Rbm27
Name: RNA binding motif protein 27
Synonyms: Psc1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225432
VEGA: 18
Homologene: 35410
Esyt2
Name: extended synaptotagmin-like protein 2
Synonyms: 2310058N22Rik, Fam62b, 4921504I16Rik, D12Ertd551e, 2410017M09Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 52635
VEGA: 12
Homologene: 32699
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100683
Homologene: 39246
Actn1
Name: actinin, alpha 1
Synonyms: 3110023F10Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 109711
VEGA: 12
HGNC: HGNC:163
Homologene: 55553
Nefl
Name: neurofilament, light polypeptide
Synonyms: NF-L, Nfl, CMT2E, NF68
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18039
HGNC: HGNC:7739
Homologene: 4487
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ankyrin-G, Ank-3, Ankyrin-3, 2900054D09Rik, AnkG
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Psmg1
Name: proteasome (prosome, macropain) assembly chaperone 1
Synonyms: Dscr2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56088
HGNC: HGNC:3043
Homologene: 2759
Pbrm1
Name: polybromo 1
Synonyms: BAF180, Pb1, 2310032M22Rik, 2610016F04Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66923
Homologene: 10044
Tmem130
Name: transmembrane protein 130
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243339
Homologene: 17680
Chrm5
Name: cholinergic receptor, muscarinic 5
Synonyms: M5R, muscarinic acetylcholine receptor 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 213788
HGNC: HGNC:1954
Homologene: 22697
Trpc1
Name: transient receptor potential cation channel, subfamily C, member 1
Synonyms: Trrp1, Mtrp1, Trp1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22063
Homologene: 2478
Lama2
Name: laminin, alpha 2
Synonyms: mer, merosin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16773
HGNC: HGNC:6482
Homologene: 37306
Naip5
Name: NLR family, apoptosis inhibitory protein 5
Synonyms: Naip-rs3, Lgn1, Birc1e
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17951
HGNC: HGNC:7634
Homologene: 113589
Il11ra1
Name: interleukin 11 receptor subunit alpha 1
Synonyms: Il-11ra, Il11ra, NR1, Il-11ra-alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16157
HGNC: HGNC:5967
Homologene: 88705
Polq
Name: polymerase (DNA directed), theta
Synonyms: A430110D14Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 77782
HGNC: HGNC:9186
Homologene: 32727
Brinp3
Name: bone morphogenetic protein/retinoic acid inducible neural specific 3
Synonyms: Fam5c, B830045N13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215378
Homologene: 17786
1110002E22Rik
Name: RIKEN cDNA 1110002E22 gene
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 102634333
Homologene: 131709
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242627
Homologene: 135888
Oog4
Name: oogenesin 4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242737
Vrtn
Name: vertebrae development associated
Synonyms: 7420416P09Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 432677
VEGA: 12
Homologene: 41236
Pls1
Name: plastin 1 (I-isoform)
Synonyms: I-fimbrin
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102502
HGNC: HGNC:9090
Homologene: 68270
Atosa
Name: atos homolog A
Synonyms: C130047D21Rik, Fam214a, BC031353, 6330415I01Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235493
VEGA: 9
Homologene: 35065
Ptprt
Name: protein tyrosine phosphatase receptor type T
Synonyms: RPTPrho
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19281
HGNC: HGNC:9682
Homologene: 56924
Dnm3
Name: dynamin 3
Synonyms: 9630020E24Rik, B230343F03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 103967
Homologene: 22906
Zfp712
Name: zinc finger protein 712
Synonyms: 4921504N20Rik, mszf89, mszf31
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 78251
VEGA: 13
Homologene: 136296
Inhbb
Name: inhibin beta-B
Synonyms: activin beta-B
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16324
HGNC: HGNC:6067
Homologene: 1654
Adam2
Name: a disintegrin and metallopeptidase domain 2
Synonyms: fertilin beta, Ftnb, Ph30-beta
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 11495
VEGA: 14
HGNC: HGNC:198
Homologene: 1127
Lrit2
Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 2
Synonyms: A930010E21Rik, Lrrc22
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239038
VEGA: 14
Homologene: 18292
Or7e168
Name: olfactory receptor family 7 subfamily E member 168
Synonyms: MOR146-3, GA_x6K02T2PVTD-13548326-13549255, MOR146-10_p, Olfr859
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258519
Homologene: 134093
Or5p79
Name: olfactory receptor family 5 subfamily P member 79
Synonyms: MOR204-7, GA_x6K02T2PBJ9-10951546-10952496, Olfr507
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258738
Homologene: 27249
Ldhb
Name: lactate dehydrogenase B
Synonyms: H-Ldh, lactate dehydrogenase-B, Ldh-2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16832
HGNC: HGNC:6541
Homologene: 55647
Cd163
Name: CD163 antigen
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 93671
HGNC: HGNC:1631
Homologene: 128811
Or1e1
Name: olfactory receptor family 1 subfamily E member 1
Synonyms: GA_x6K02T2P1NL-3514066-3515010, MOR135-11, MTPCR06, Olfr21, MTPCR55, Olfr20
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258925
Homologene: 128054
B4galt4
Name: UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 4
Synonyms: 9130402O08Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 56375
HGNC: HGNC:927
Homologene: 37848
Ifi214
Name: interferon activated gene 214
Synonyms: Pyhin-B, BC094916
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 545384
HGNC: HGNC:5395
Homologene: 115929
Stx17
Name: syntaxin 17
Synonyms: 9030425C21Rik, 6330411F21Rik, 4833418L03Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67727
Homologene: 9917
Dctn5
Name: dynactin 5
Synonyms: p25 dynactin subunit, b2b315Clo, 4930427E12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 59288
Homologene: 10998
Wdr55
Name: WD repeat domain 55
Synonyms: 2410080P20Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67936
VEGA: 18
Homologene: 9789
Vsx1
Name: visual system homeobox 1
Synonyms: CHX10-like
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 114889
Homologene: 8743
Or6c216
Name: olfactory receptor family 6 subfamily C member 216
Synonyms: Olfr812, MOR110-1, GA_x6K02T2PULF-11521598-11520666
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258791
Homologene: 73931
Tas2r114
Name: taste receptor, type 2, member 114
Synonyms: Tas2r14, T2R14, mGR14, mt2r46
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 387346
Homologene: 128355
Malrd1
Name: MAM and LDL receptor class A domain containing 1
Synonyms: Diet1, Gm13364, Gm13318
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 102635496
Homologene: 136214
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 119,417,339 bp
  • A to T, chromosome 1 at 146,901,693 bp
  • A to T, chromosome 1 at 162,313,595 bp
  • C to A, chromosome 1 at 173,525,031 bp
  • T to A, chromosome 2 at 15,931,689 bp
  • A to T, chromosome 2 at 112,480,480 bp
  • G to T, chromosome 2 at 150,688,521 bp
  • A to G, chromosome 2 at 161,534,587 bp
  • T to G, chromosome 3 at 36,570,963 bp
  • C to T, chromosome 3 at 138,068,800 bp
  • C to T, chromosome 4 at 41,765,412 bp
  • T to A, chromosome 4 at 48,183,478 bp
  • T to G, chromosome 4 at 113,539,355 bp
  • A to C, chromosome 4 at 143,437,978 bp
  • T to A, chromosome 5 at 144,752,414 bp
  • T to A, chromosome 5 at 144,857,002 bp
  • G to A, chromosome 6 at 124,304,744 bp
  • A to T, chromosome 6 at 131,689,136 bp
  • A to T, chromosome 6 at 142,494,121 bp
  • G to T, chromosome 7 at 108,622,506 bp
  • G to A, chromosome 7 at 122,135,097 bp
  • T to C, chromosome 8 at 18,694,517 bp
  • A to G, chromosome 9 at 19,808,551 bp
  • C to A, chromosome 9 at 75,023,648 bp
  • T to C, chromosome 9 at 95,726,437 bp
  • A to G, chromosome 9 at 95,754,745 bp
  • T to A, chromosome 10 at 27,031,158 bp
  • T to C, chromosome 10 at 69,991,744 bp
  • T to C, chromosome 10 at 129,842,584 bp
  • T to A, chromosome 11 at 73,354,185 bp
  • C to T, chromosome 12 at 80,168,417 bp
  • T to G, chromosome 12 at 84,650,316 bp
  • A to G, chromosome 12 at 116,321,170 bp
  • A to T, chromosome 13 at 67,052,336 bp
  • A to G, chromosome 13 at 100,221,594 bp
  • A to G, chromosome 14 at 31,061,509 bp
  • T to C, chromosome 14 at 37,068,810 bp
  • C to T, chromosome 14 at 66,058,790 bp
  • A to G, chromosome 14 at 68,084,585 bp
  • T to C, chromosome 16 at 37,060,827 bp
  • T to A, chromosome 16 at 38,757,822 bp
  • A to G, chromosome 16 at 95,988,097 bp
  • T to C, chromosome 18 at 36,762,178 bp
  • T to A, chromosome 18 at 42,337,011 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6499 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044631-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.