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Strain Name:
C57BL/6J-MtgxR6499Btlr/Mmmh
Stock Number:
044631-MU
Citation ID:
RRID:MMRRC_044631-MU
Other Names:
R6499 (G1)
Major Collection:

Strain Information

Angpt2
Name: angiopoietin 2
Synonyms: Ang2, Ang-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11601
HGNC: HGNC:485
Homologene: 22401
Ccna2
Name: cyclin A2
Synonyms: Cyca, Ccn1, Ccn-1, Ccna, CycA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12428
HGNC: HGNC:1578
Homologene: 55562
Rbm27
Name: RNA binding motif protein 27
Synonyms: Psc1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225432
VEGA: 18
Homologene: 35410
Esyt2
Name: extended synaptotagmin-like protein 2
Synonyms: 2410017M09Rik, 4921504I16Rik, 2310058N22Rik, D12Ertd551e, Fam62b
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 52635
VEGA: 12
Homologene: 32699
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100683
Homologene: 39246
Actn1
Name: actinin, alpha 1
Synonyms: 3110023F10Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 109711
VEGA: 12
HGNC: HGNC:163
Homologene: 55553
Nefl
Name: neurofilament, light polypeptide
Synonyms: NF-L, CMT2E, NF68, Nfl
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18039
HGNC: HGNC:7739
Homologene: 4487
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 119,417,339 bp
  • A to T, chromosome 1 at 146,901,693 bp
  • A to T, chromosome 1 at 162,313,595 bp
  • C to A, chromosome 1 at 173,525,031 bp
  • T to A, chromosome 2 at 15,931,689 bp
  • A to T, chromosome 2 at 112,480,480 bp
  • G to T, chromosome 2 at 150,688,521 bp
  • A to G, chromosome 2 at 161,534,587 bp
  • T to G, chromosome 3 at 36,570,963 bp
  • C to T, chromosome 3 at 138,068,800 bp
  • C to T, chromosome 4 at 41,765,412 bp
  • T to A, chromosome 4 at 48,183,478 bp
  • T to G, chromosome 4 at 113,539,355 bp
  • A to C, chromosome 4 at 143,437,978 bp
  • T to A, chromosome 5 at 144,752,414 bp
  • T to A, chromosome 5 at 144,857,002 bp
  • G to A, chromosome 6 at 124,304,744 bp
  • A to T, chromosome 6 at 131,689,136 bp
  • A to T, chromosome 6 at 142,494,121 bp
  • G to T, chromosome 7 at 108,622,506 bp
  • G to A, chromosome 7 at 122,135,097 bp
  • T to C, chromosome 8 at 18,694,517 bp
  • A to G, chromosome 9 at 19,808,551 bp
  • C to A, chromosome 9 at 75,023,648 bp
  • T to C, chromosome 9 at 95,726,437 bp
  • A to G, chromosome 9 at 95,754,745 bp
  • T to A, chromosome 10 at 27,031,158 bp
  • T to C, chromosome 10 at 69,991,744 bp
  • T to C, chromosome 10 at 129,842,584 bp
  • T to A, chromosome 11 at 73,354,185 bp
  • C to T, chromosome 12 at 80,168,417 bp
  • T to G, chromosome 12 at 84,650,316 bp
  • A to G, chromosome 12 at 116,321,170 bp
  • A to T, chromosome 13 at 67,052,336 bp
  • A to G, chromosome 13 at 100,221,594 bp
  • A to G, chromosome 14 at 31,061,509 bp
  • T to C, chromosome 14 at 37,068,810 bp
  • C to T, chromosome 14 at 66,058,790 bp
  • A to G, chromosome 14 at 68,084,585 bp
  • T to C, chromosome 16 at 37,060,827 bp
  • T to A, chromosome 16 at 38,757,822 bp
  • A to G, chromosome 16 at 95,988,097 bp
  • T to C, chromosome 18 at 36,762,178 bp
  • T to A, chromosome 18 at 42,337,011 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6499 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044631-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.