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Strain Name:
C57BL/6J-MtgxR6505Btlr/Mmmh
Stock Number:
044637-MU
Citation ID:
RRID:MMRRC_044637-MU
Other Names:
R6505 (G1)
Major Collection:

Strain Information

Lamb1
Name: laminin B1
Synonyms: Lamb-1, C81607, C80098, D130003D08Rik, Lamb1-1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16777
HGNC: HGNC:6486
Homologene: 1722
Prkacb
Name: protein kinase, cAMP dependent, catalytic, beta
Synonyms: cAMP-dependent protein kinase C beta, Pkacb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18749
HGNC: HGNC:9381
Homologene: 121718
Aldh7a1
Name: aldehyde dehydrogenase family 7, member A1
Synonyms: Atq1, D18Wsu181e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 110695
HGNC: HGNC:877
Homologene: 913
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: A330076K04Rik, 1100001C18Rik, 2500002E12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70097
Homologene: 69182
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Hcrtr1
Name: hypocretin (orexin) receptor 1
Synonyms: OX1R
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230777
HGNC: HGNC:4848
Homologene: 37492
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: p1.m, P1, Mcmd
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,803,544 bp
  • C to T, chromosome 1 at 24,014,872 bp
  • A to T, chromosome 1 at 40,489,707 bp
  • T to C, chromosome 1 at 75,406,684 bp
  • C to A, chromosome 1 at 75,429,523 bp
  • T to C, chromosome 1 at 123,336,851 bp
  • C to A, chromosome 1 at 132,316,394 bp
  • T to C, chromosome 1 at 151,360,993 bp
  • T to A, chromosome 1 at 169,933,023 bp
  • T to C, chromosome 1 at 183,086,512 bp
  • G to A, chromosome 2 at 32,222,320 bp
  • A to T, chromosome 2 at 62,546,603 bp
  • T to A, chromosome 2 at 87,457,927 bp
  • A to T, chromosome 2 at 87,943,174 bp
  • A to T, chromosome 2 at 93,668,559 bp
  • A to T, chromosome 2 at 119,451,441 bp
  • A to G, chromosome 2 at 120,534,502 bp
  • A to T, chromosome 3 at 60,086,723 bp
  • A to G, chromosome 3 at 90,267,611 bp
  • T to G, chromosome 3 at 106,822,322 bp
  • T to A, chromosome 3 at 144,825,259 bp
  • T to A, chromosome 3 at 146,732,646 bp
  • C to T, chromosome 3 at 152,481,669 bp
  • T to A, chromosome 4 at 104,512,264 bp
  • G to A, chromosome 4 at 126,463,835 bp
  • T to A, chromosome 4 at 130,137,586 bp
  • C to T, chromosome 5 at 24,390,577 bp
  • A to G, chromosome 5 at 43,820,590 bp
  • T to A, chromosome 5 at 87,980,663 bp
  • A to T, chromosome 5 at 107,403,656 bp
  • A to G, chromosome 5 at 117,351,759 bp
  • A to G, chromosome 5 at 127,784,438 bp
  • C to T, chromosome 6 at 24,954,921 bp
  • C to T, chromosome 6 at 116,540,600 bp
  • A to G, chromosome 6 at 122,850,640 bp
  • G to A, chromosome 7 at 3,661,212 bp
  • A to C, chromosome 7 at 24,744,246 bp
  • T to A, chromosome 7 at 47,235,136 bp
  • C to A, chromosome 7 at 80,393,617 bp
  • C to A, chromosome 7 at 83,951,597 bp
  • G to A, chromosome 7 at 97,579,910 bp
  • C to A, chromosome 7 at 103,309,793 bp
  • T to A, chromosome 7 at 103,492,651 bp
  • T to C, chromosome 7 at 108,103,567 bp
  • C to T, chromosome 7 at 143,565,007 bp
  • A to G, chromosome 8 at 15,042,304 bp
  • A to G, chromosome 8 at 71,355,857 bp
  • T to C, chromosome 9 at 19,374,941 bp
  • T to C, chromosome 9 at 42,071,234 bp
  • A to T, chromosome 9 at 64,307,513 bp
  • T to A, chromosome 9 at 79,647,605 bp
  • T to A, chromosome 9 at 108,496,883 bp
  • C to G, chromosome 10 at 8,729,527 bp
  • C to T, chromosome 10 at 76,449,281 bp
  • G to A, chromosome 10 at 77,559,673 bp
  • A to T, chromosome 10 at 80,543,011 bp
  • C to A, chromosome 11 at 50,199,852 bp
  • A to T, chromosome 11 at 65,693,529 bp
  • T to C, chromosome 11 at 68,213,199 bp
  • C to T, chromosome 11 at 68,492,789 bp
  • T to A, chromosome 11 at 70,250,204 bp
  • A to G, chromosome 11 at 77,691,613 bp
  • T to A, chromosome 11 at 87,106,124 bp
  • T to C, chromosome 11 at 100,757,085 bp
  • T to A, chromosome 11 at 101,523,541 bp
  • A to G, chromosome 11 at 102,276,477 bp
  • A to T, chromosome 12 at 31,323,462 bp
  • T to C, chromosome 12 at 64,966,590 bp
  • G to T, chromosome 13 at 27,773,793 bp
  • C to T, chromosome 13 at 43,668,671 bp
  • A to G, chromosome 14 at 44,331,371 bp
  • T to C, chromosome 14 at 55,973,779 bp
  • T to C, chromosome 14 at 59,277,537 bp
  • G to A, chromosome 15 at 66,759,558 bp
  • A to T, chromosome 16 at 16,673,920 bp
  • A to G, chromosome 16 at 46,448,821 bp
  • C to T, chromosome 16 at 91,499,537 bp
  • A to T, chromosome 17 at 9,001,940 bp
  • G to A, chromosome 17 at 44,085,264 bp
  • T to C, chromosome 18 at 12,495,348 bp
  • C to A, chromosome 18 at 33,464,305 bp
  • T to A, chromosome 18 at 37,320,880 bp
  • A to T, chromosome 18 at 46,505,875 bp
  • C to A, chromosome 18 at 52,906,537 bp
  • T to C, chromosome 18 at 56,526,996 bp
  • A to G, chromosome 18 at 61,129,733 bp
  • A to G, chromosome 18 at 86,498,574 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6505 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044637-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.