Strain Name:
C57BL/6J-MtgxR6514Btlr/Mmmh
Stock Number:
044641-MU
Citation ID:
RRID:MMRRC_044641-MU
Other Names:
R6514 (G1)
Major Collection:

Strain Information

Irf1
Name: interferon regulatory factor 1
Synonyms: Irf-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16362
HGNC: HGNC:6116
Homologene: 1658
Plcb4
Name: phospholipase C, beta 4
Synonyms: A930039J07Rik, C230058B11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18798
HGNC: HGNC:9059
Homologene: 8471
Add1
Name: adducin 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11518
HGNC: HGNC:243
Homologene: 22758
Cdc6
Name: cell division cycle 6
Synonyms: CDC18(S.pombe), CDC18L, cell division cycle 18 homolog (S.pombe)-like
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 23834
HGNC: HGNC:1744
Homologene: 68172
Eif4enif1
Name: eukaryotic translation initiation factor 4E nuclear import factor 1
Synonyms: Clast4, D11Ertd166e, 2610509L04Rik, A930019J01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74203
Homologene: 10522
Arrdc3
Name: arrestin domain containing 3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 105171
Homologene: 69318
Ufl1
Name: UFM1 specific ligase 1
Synonyms: Maxer, 1810074P20Rik, Rcad
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67490
Homologene: 9093
Pgd
Name: phosphogluconate dehydrogenase
Synonyms: 0610042A05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110208
HGNC: HGNC:8891
Homologene: 68268
Pdcd6ip
Name: programmed cell death 6 interacting protein
Synonyms: AIP1, Alix
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18571
HGNC: HGNC:8766
Homologene: 22614
Mmp16
Name: matrix metallopeptidase 16
Synonyms: MT3-MMP, Membrane type 3-MMP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17389
HGNC: HGNC:7162
Homologene: 55939
Mme
Name: membrane metallo endopeptidase
Synonyms: NEP, CD10, 6030454K05Rik, neprilysin, neutral endopeptidase
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17380
HGNC: HGNC:7154
Homologene: 5275
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: Ip3r3, Itpr-3, tf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Skor2
Name: SKI family transcriptional corepressor 2
Synonyms: Fussel18, Corl2, Gm7348
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 664805
VEGA: 18
Homologene: 64797
Ryr1
Name: ryanodine receptor 1, skeletal muscle
Synonyms: skrr, Ryr, calcium release channel isoform 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20190
Homologene: 68069
Vav1
Name: vav 1 oncogene
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22324
Homologene: 3961
Cntnap5c
Name: contactin associated protein-like 5C
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 620292
VEGA: 17
Homologene: 128806
Capn7
Name: calpain 7
Synonyms: PalBH
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12339
HGNC: HGNC:1484
Homologene: 7651
Gm10801
Name: predicted gene 10801
Type: Gene
Species: Mouse
Chromosome: 2
Mfsd13a
Name: major facilitator superfamily domain containing 13a
Synonyms: Tmem180, 4930449A08Rik, 4930538D17Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 75146
Homologene: 12592
Crybg1
Name: crystallin beta-gamma domain containing 1
Synonyms: Aim1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11630
HGNC: HGNC:356
Homologene: 18168
Ngp
Name: neutrophilic granule protein
Synonyms: clone B6, bectenecin, myeloid granule protein
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18054
Homologene: 49180
Erbb2
Name: erb-b2 receptor tyrosine kinase 2
Synonyms: c-neu, HER-2, Neu, Neu oncogene, ErbB-2, l11Jus8, c-erbB2, HER2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13866
HGNC: HGNC:3430
Homologene: 3273
Serpine2
Name: serine (or cysteine) peptidase inhibitor, clade E, member 2
Synonyms: PN-1, Spi4, nexin, PI7, B230326M24Rik, protease nexin 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20720
HGNC: HGNC:8951
Homologene: 21247
Or2q1
Name: olfactory receptor family 2 subfamily Q member 1
Synonyms: GA_x6K02T2P3E9-4742413-4741481, Olfr450, MOR257-3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258437
Homologene: 51720
Gfm1
Name: G elongation factor, mitochondrial 1
Synonyms: D3Wsu133e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 28030
Homologene: 6449
Tle6
Name: transducin-like enhancer of split 6
Synonyms: Grg6, 1810057E06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 114606
Homologene: 11701
H2-M11
Name: histocompatibility 2, M region locus 11
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224754
Homologene: 52241
Apol7b
Name: apolipoprotein L 7b
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 278679
HGNC: HGNC:619
Homologene: 40940
Duoxa1
Name: dual oxidase maturation factor 1
Synonyms: Nip1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 213696
Homologene: 16043
Ppl
Name: periplakin
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19041
VEGA: 16
HGNC: HGNC:9273
Homologene: 2026
Ly6g
Name: lymphocyte antigen 6 family member G
Synonyms: Ly-6G, Gr1, Gr-1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 546644
Homologene: 113718
Ech1
Name: enoyl coenzyme A hydratase 1, peroxisomal
Synonyms: dienoyl-CoA isomerase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 51798
HGNC: HGNC:3149
Homologene: 1069
Egr3
Name: early growth response 3
Synonyms: Pilot
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13655
VEGA: 14
HGNC: HGNC:3240
Homologene: 37923
4933414I15Rik
Name: RIKEN cDNA 4933414I15 gene
Synonyms: ENSMUSG00000051192
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74408
Fer1l5
Name: fer-1 like family member 5
Synonyms: 4930533C12Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100534273
Homologene: 85232
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,403,616 bp
  • A to C, chromosome 1 at 79,821,570 bp
  • T to A, chromosome 2 at 98,663,869 bp
  • A to G, chromosome 2 at 122,304,713 bp
  • T to A, chromosome 2 at 135,954,996 bp
  • T to A, chromosome 3 at 63,364,844 bp
  • T to C, chromosome 3 at 67,473,546 bp
  • T to C, chromosome 4 at 18,116,123 bp
  • T to A, chromosome 4 at 25,262,238 bp
  • C to T, chromosome 4 at 149,160,752 bp
  • T to A, chromosome 5 at 34,605,973 bp
  • T to A, chromosome 6 at 42,817,996 bp
  • A to G, chromosome 7 at 28,826,015 bp
  • A to G, chromosome 7 at 29,046,841 bp
  • A to T, chromosome 9 at 110,419,949 bp
  • G to A, chromosome 9 at 113,689,694 bp
  • A to T, chromosome 10 at 43,997,215 bp
  • G to A, chromosome 10 at 81,591,976 bp
  • T to A, chromosome 11 at 3,240,996 bp
  • G to A, chromosome 11 at 50,942,742 bp
  • C to G, chromosome 11 at 53,771,322 bp
  • A to G, chromosome 11 at 98,420,146 bp
  • A to G, chromosome 11 at 98,919,292 bp
  • T to C, chromosome 12 at 115,593,120 bp
  • A to G, chromosome 13 at 80,889,190 bp
  • T to G, chromosome 14 at 31,344,554 bp
  • T to C, chromosome 14 at 70,078,917 bp
  • C to T, chromosome 15 at 75,156,732 bp
  • C to T, chromosome 15 at 77,423,926 bp
  • A to G, chromosome 16 at 5,087,317 bp
  • C to T, chromosome 17 at 27,091,370 bp
  • A to T, chromosome 17 at 36,548,947 bp
  • T to C, chromosome 17 at 57,327,660 bp
  • A to G, chromosome 17 at 58,330,170 bp
  • T to A, chromosome 18 at 76,862,694 bp
  • T to C, chromosome 19 at 46,374,625 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6514 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044641-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.