Strain Name:
C57BL/6J-MtgxR6517Btlr/Mmmh
Stock Number:
044644-MU
Citation ID:
RRID:MMRRC_044644-MU
Other Names:
R6517 (G1)
Major Collection:

Strain Information

Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: bt, ADAMTS-20
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 4930431J08Rik, 9330107J05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Senp2
Name: SUMO/sentrin specific peptidase 2
Synonyms: 4930538C18Rik, 2310007L05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 75826
VEGA: 16
Homologene: 11005
Fbxo38
Name: F-box protein 38
Synonyms: SP329, 6030410I24Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 107035
VEGA: 18
Homologene: 34526
Slu7
Name: SLU7 splicing factor homolog (S. cerevisiae)
Synonyms: D3Bwg0878e, D11Ertd730e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 193116
Homologene: 4690
Actr8
Name: ARP8 actin-related protein 8
Synonyms: ARP8, 5730542K05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 56249
VEGA: 14
Homologene: 11286
Stt3b
Name: STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Synonyms: 1300006C19Rik, Simp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68292
VEGA: 9
Homologene: 7387
Taf1c
Name: TATA-box binding protein associated factor, RNA polymerase I, C
Synonyms: mTAFI95
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 21341
Homologene: 21163
Glul
Name: glutamate-ammonia ligase
Synonyms: GS, Glns
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14645
HGNC: HGNC:4341
Homologene: 37567
Tnks1bp1
Name: tankyrase 1 binding protein 1
Synonyms: TAB182
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228140
Homologene: 14117
Epha5
Name: Eph receptor A5
Synonyms: Rek7, Cek7, bsk, Hek7, Ehk1, Els1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13839
HGNC: HGNC:3389
Homologene: 55824
Ets1
Name: E26 avian leukemia oncogene 1, 5' domain
Synonyms: Tpl1, Ets-1, p51Ets-1, p42Ets-1, vs
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23871
HGNC: HGNC:3488
Homologene: 3837
Nos3
Name: nitric oxide synthase 3, endothelial cell
Synonyms: ecNOS, 2310065A03Rik, eNOS, Nos-3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18127
HGNC: HGNC:7876
Homologene: 504
Myo1g
Name: myosin IG
Synonyms: E430002D17Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 246177
Homologene: 27996
Zfp608
Name: zinc finger protein 608
Synonyms: 4932417D18Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 269023
VEGA: 18
Homologene: 18485
Sis
Name: sucrase isomaltase
Synonyms: sucrase-isomaltase, 2010204N08Rik, Si-s
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69983
Homologene: 37424
Fscn1
Name: fascin actin-bundling protein 1
Synonyms: Fan1, fascin-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14086
Homologene: 48164
Cep162
Name: centrosomal protein 162
Synonyms: 4922501C03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382090
Homologene: 8930
Alpk3
Name: alpha-kinase 3
Synonyms: Midori
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 116904
Homologene: 10813
Keg1
Name: kidney expressed gene 1
Synonyms: 0610008P16Rik, GS4059
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 64697
Homologene: 69461
Or8h8
Name: olfactory receptor family 8 subfamily H member 8
Synonyms: Olfr1098, MOR206-1, GA_x6K02T2Q125-48410458-48409511
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258842
Homologene: 37005
Zfp986
Name: zinc finger protein 986
Synonyms: Gm13235
Type: Gene
Species: Mouse
Chromosome: 4
Tkt
Name: transketolase
Synonyms: p68
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 21881
Homologene: 68154
Piwil2
Name: piwi-like RNA-mediated gene silencing 2
Synonyms: Miwi like, mili
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57746
VEGA: 14
Homologene: 23071
Ppm1l
Name: protein phosphatase 1 (formerly 2C)-like
Synonyms: 5930404J21Rik, PP2C-epsilon, Pp2ce
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242083
Homologene: 33158
Slc22a22
Name: solute carrier family 22 (organic cation transporter), member 22
Synonyms: BC026439, OAT-PG
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 210463
Homologene: 18166
Tle6
Name: transducin-like enhancer of split 6
Synonyms: 1810057E06Rik, Grg6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 114606
Homologene: 11701
Krt1
Name: keratin 1
Synonyms: Krt-2.1, Krt2-1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16678
VEGA: 15
HGNC: HGNC:6412
Homologene: 38146
Mdfic
Name: MyoD family inhibitor domain containing
Synonyms: Kdt1, clone 1.5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16543
Homologene: 18404
Tcirg1
Name: T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3
Synonyms: V-ATPase a3, OC-116, Atp6i, TIRC7, ATP6a3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 27060
Homologene: 4392
Stra6l
Name: STRA6-like
Synonyms: 1300002K09Rik, Rbpr2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74152
Homologene: 81924
Sgo2b
Name: shugoshin 2B
Synonyms: Gm4975, Sgol2b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244495
Homologene: 51867
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 63,305,520 bp
  • A to G, chromosome 1 at 153,908,033 bp
  • T to A, chromosome 2 at 65,497,563 bp
  • T to C, chromosome 2 at 85,059,345 bp
  • A to G, chromosome 2 at 86,923,097 bp
  • T to C, chromosome 3 at 69,317,583 bp
  • T to C, chromosome 3 at 72,907,142 bp
  • A to G, chromosome 4 at 45,879,473 bp
  • G to C, chromosome 4 at 145,899,300 bp
  • T to A, chromosome 5 at 24,383,624 bp
  • T to C, chromosome 5 at 84,156,501 bp
  • A to G, chromosome 5 at 142,971,986 bp
  • T to A, chromosome 6 at 15,770,325 bp
  • T to A, chromosome 7 at 81,078,579 bp
  • A to T, chromosome 8 at 63,931,494 bp
  • T to G, chromosome 8 at 119,604,247 bp
  • A to T, chromosome 9 at 32,752,797 bp
  • T to A, chromosome 9 at 87,222,174 bp
  • T to C, chromosome 9 at 115,267,342 bp
  • G to A, chromosome 10 at 81,591,976 bp
  • T to C, chromosome 11 at 6,512,509 bp
  • T to A, chromosome 11 at 43,438,148 bp
  • C to T, chromosome 14 at 29,982,716 bp
  • A to G, chromosome 14 at 30,549,323 bp
  • G to T, chromosome 14 at 70,374,336 bp
  • A to G, chromosome 15 at 57,250,969 bp
  • A to C, chromosome 15 at 94,283,104 bp
  • A to T, chromosome 15 at 101,850,267 bp
  • C to T, chromosome 16 at 22,026,724 bp
  • A to T, chromosome 18 at 54,899,078 bp
  • C to T, chromosome 18 at 62,533,563 bp
  • C to T, chromosome 19 at 3,901,933 bp
  • A to G, chromosome 19 at 12,715,910 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6517 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044644-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.