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Strain Name:
C57BL/6J-MtgxR6526Btlr/Mmmh
Stock Number:
044652-MU
Citation ID:
RRID:MMRRC_044652-MU
Other Names:
R6526 (G1)
Major Collection:

Strain Information

Pitx2
Name: paired-like homeodomain transcription factor 2
Synonyms: Otlx2, Brx1b, Brx1a, Brx1, solurshin, Ptx2, Munc30, Pitx2c, Pitx2b, Pitx2a, Rieg
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18741
HGNC: HGNC:9005
Homologene: 55454
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Gtf2h3
Name: general transcription factor IIH, polypeptide 3
Synonyms: 5033417D07Rik, BTF2, D5Ertd679e, 34kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209357
HGNC: HGNC:4657
Homologene: 1160
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Trmt13
Name: tRNA methyltransferase 13
Synonyms: A930028L21Rik, 4631408H19Rik, Ccdc76
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229780
Homologene: 6875
Setd2
Name: SET domain containing 2
Synonyms: 4921524K10Rik, KMT3A
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235626
Homologene: 56493
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,284,172 bp
  • A to T, chromosome 1 at 80,586,351 bp
  • A to T, chromosome 1 at 82,340,659 bp
  • T to C, chromosome 1 at 87,676,250 bp
  • G to A, chromosome 1 at 88,361,998 bp
  • A to T, chromosome 1 at 128,300,478 bp
  • T to A, chromosome 1 at 131,841,149 bp
  • A to G, chromosome 1 at 133,711,729 bp
  • A to G, chromosome 2 at 103,439,233 bp
  • T to A, chromosome 2 at 128,672,135 bp
  • A to G, chromosome 2 at 155,851,423 bp
  • T to C, chromosome 3 at 15,379,020 bp
  • A to T, chromosome 3 at 80,692,469 bp
  • T to A, chromosome 3 at 116,592,215 bp
  • T to C, chromosome 3 at 129,214,783 bp
  • T to A, chromosome 3 at 157,567,502 bp
  • A to G, chromosome 4 at 49,383,497 bp
  • T to C, chromosome 4 at 56,798,812 bp
  • A to T, chromosome 4 at 106,734,004 bp
  • A to G, chromosome 4 at 143,852,814 bp
  • T to A, chromosome 5 at 48,304,167 bp
  • T to A, chromosome 5 at 88,154,050 bp
  • T to A, chromosome 5 at 110,204,895 bp
  • T to C, chromosome 5 at 110,848,690 bp
  • C to A, chromosome 5 at 122,961,469 bp
  • C to T, chromosome 5 at 124,584,297 bp
  • G to T, chromosome 5 at 135,835,234 bp
  • T to A, chromosome 5 at 137,290,644 bp
  • T to C, chromosome 5 at 138,163,900 bp
  • C to T, chromosome 5 at 145,455,895 bp
  • C to T, chromosome 6 at 24,097,612 bp
  • C to A, chromosome 6 at 71,929,443 bp
  • T to G, chromosome 6 at 73,074,704 bp
  • T to A, chromosome 6 at 131,221,876 bp
  • A to T, chromosome 7 at 8,378,099 bp
  • T to A, chromosome 7 at 10,447,671 bp
  • T to C, chromosome 7 at 56,157,330 bp
  • A to G, chromosome 7 at 67,735,583 bp
  • T to A, chromosome 7 at 81,361,669 bp
  • A to G, chromosome 7 at 107,967,462 bp
  • G to A, chromosome 8 at 9,987,777 bp
  • T to C, chromosome 8 at 12,864,999 bp
  • T to C, chromosome 8 at 83,319,457 bp
  • A to T, chromosome 8 at 91,096,839 bp
  • T to C, chromosome 8 at 110,881,786 bp
  • A to C, chromosome 8 at 122,576,816 bp
  • T to A, chromosome 9 at 20,593,871 bp
  • G to A, chromosome 9 at 110,532,717 bp
  • A to G, chromosome 10 at 59,429,640 bp
  • A to G, chromosome 10 at 80,232,279 bp
  • G to T, chromosome 10 at 107,542,653 bp
  • A to G, chromosome 10 at 127,056,689 bp
  • T to C, chromosome 10 at 128,911,489 bp
  • G to A, chromosome 11 at 35,661,292 bp
  • C to T, chromosome 11 at 35,853,437 bp
  • T to A, chromosome 11 at 61,509,097 bp
  • T to C, chromosome 11 at 68,949,994 bp
  • C to T, chromosome 11 at 68,973,688 bp
  • C to T, chromosome 11 at 94,359,372 bp
  • A to G, chromosome 11 at 94,494,932 bp
  • A to T, chromosome 12 at 13,405,425 bp
  • T to C, chromosome 12 at 73,702,775 bp
  • T to G, chromosome 12 at 84,313,952 bp
  • T to A, chromosome 13 at 64,781,888 bp
  • G to A, chromosome 15 at 44,498,089 bp
  • T to C, chromosome 15 at 58,344,957 bp
  • A to G, chromosome 16 at 38,447,276 bp
  • G to A, chromosome 16 at 45,239,094 bp
  • G to T, chromosome 16 at 48,587,124 bp
  • A to T, chromosome 17 at 46,164,111 bp
  • C to A, chromosome 17 at 73,900,551 bp
  • A to G, chromosome 18 at 86,498,748 bp
  • T to C, chromosome 19 at 4,604,495 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6526 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044652-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.