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Strain Name:
C57BL/6J-MtgxR6552Btlr/Mmmh
Stock Number:
044677-MU
Citation ID:
RRID:MMRRC_044677-MU
Other Names:
R6552 (G1)
Major Collection:

Strain Information

Dnmt3a
Name: DNA methyltransferase 3A
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13435
HGNC: HGNC:2978
Homologene: 7294
Lcat
Name: lecithin cholesterol acyltransferase
Synonyms: D8Wsu61e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16816
HGNC: HGNC:6522
Homologene: 68042
Epb41l4a
Name: erythrocyte membrane protein band 4.1 like 4a
Synonyms: NBL4, Epb4.1l4a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13824
VEGA: 18
Homologene: 8398
Efr3a
Name: EFR3 homolog A
Synonyms: A130089M23Rik, D030063F01Rik, C920006C10Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76740
Homologene: 44222
Stam2
Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Synonyms: Hbp, 1200004O12Rik, 5730456G07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56324
Homologene: 68490
Golga4
Name: golgin A4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Sec24d
Name: SEC24 homolog D, COPII coat complex component
Synonyms: 2310020L09Rik, LOC383951
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69608
Homologene: 40986
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 24,005,606 bp
  • T to C, chromosome 2 at 52,708,227 bp
  • G to A, chromosome 2 at 153,987,158 bp
  • G to T, chromosome 2 at 180,181,154 bp
  • A to G, chromosome 3 at 54,782,746 bp
  • A to G, chromosome 3 at 123,290,552 bp
  • C to T, chromosome 4 at 6,272,361 bp
  • T to C, chromosome 4 at 112,290,285 bp
  • A to C, chromosome 4 at 113,067,490 bp
  • G to A, chromosome 5 at 3,623,953 bp
  • T to C, chromosome 5 at 111,420,887 bp
  • A to G, chromosome 6 at 53,685,384 bp
  • GATATATA to GATATATATA, chromosome 6 at 91,519,635 bp
  • A to G, chromosome 6 at 115,931,748 bp
  • A to T, chromosome 6 at 134,454,729 bp
  • C to T, chromosome 7 at 12,193,757 bp
  • G to A, chromosome 7 at 19,201,163 bp
  • TGAAGCC to T, chromosome 7 at 106,714,643 bp
  • C to T, chromosome 7 at 126,493,821 bp
  • T to C, chromosome 8 at 105,939,679 bp
  • T to A, chromosome 8 at 110,742,427 bp
  • T to C, chromosome 9 at 37,646,400 bp
  • C to T, chromosome 9 at 105,698,913 bp
  • T to C, chromosome 9 at 108,534,305 bp
  • T to A, chromosome 9 at 118,514,231 bp
  • T to C, chromosome 10 at 25,993,483 bp
  • G to T, chromosome 10 at 77,606,257 bp
  • A to T, chromosome 11 at 42,147,099 bp
  • A to G, chromosome 11 at 73,137,564 bp
  • A to G, chromosome 11 at 102,884,144 bp
  • T to C, chromosome 12 at 3,907,623 bp
  • T to G, chromosome 12 at 75,890,241 bp
  • A to G, chromosome 12 at 87,459,419 bp
  • A to G, chromosome 13 at 76,072,196 bp
  • A to T, chromosome 14 at 43,962,797 bp
  • A to T, chromosome 14 at 79,198,222 bp
  • T to C, chromosome 14 at 99,301,642 bp
  • A to G, chromosome 15 at 65,857,490 bp
  • A to G, chromosome 16 at 44,379,033 bp
  • G to A, chromosome 17 at 37,405,905 bp
  • G to T, chromosome 17 at 46,478,962 bp
  • G to T, chromosome 17 at 46,966,268 bp
  • G to A, chromosome 18 at 10,541,814 bp
  • A to G, chromosome 18 at 33,878,979 bp
  • C to A, chromosome 18 at 35,622,338 bp
  • A to T, chromosome 18 at 37,295,993 bp
  • A to G, chromosome 18 at 45,560,098 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6552 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044677-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.