Strain Name:
C57BL/6J-MtgxR6659Btlr/Mmmh
Stock Number:
044779-MU
Citation ID:
RRID:MMRRC_044779-MU
Other Names:
R6659 (G1)
Major Collection:

Strain Information

Ppp1r37
Name: protein phosphatase 1, regulatory subunit 37
Synonyms: Lrrc68
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232947
Homologene: 17851
Add1
Name: adducin 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 11518
HGNC: HGNC:243
Homologene: 22758
Acap2
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Synonyms: Centb2, 9530039J15Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78618
VEGA: 16
Homologene: 8182
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: 4930523M17Rik, 1500002J22Rik, repro8, eIF4GII, G1-419-52
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Pkn2
Name: protein kinase N2
Synonyms: Prkcl2, 6030436C20Rik, PRK2, Stk7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Ptbp3
Name: polypyrimidine tract binding protein 3
Synonyms: Rod1, 5830471K22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230257
Homologene: 55872
Atxn2
Name: ataxin 2
Synonyms: Sca2, ATX2, 9630045M23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20239
Homologene: 2234
Zgrf1
Name: zinc finger, GRF-type containing 1
Synonyms: 4930422G04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71643
Homologene: 34708
Ddx46
Name: DEAD box helicase 46
Synonyms: 8430438J23Rik, 2200005K02Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 46
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 212880
VEGA: 13
Homologene: 5430
Tmem135
Name: transmembrane protein 135
Synonyms: 2810439K08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72759
Homologene: 11295
Iars2
Name: isoleucine-tRNA synthetase 2, mitochondrial
Synonyms: 2010002H18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381314
Homologene: 7118
Prmt2
Name: protein arginine N-methyltransferase 2
Synonyms: Hrmt1l1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15468
HGNC: HGNC:5186
Homologene: 55587
Map2k3
Name: mitogen-activated protein kinase kinase 3
Synonyms: MKK3, Prkmk3, MAP kinase kinase 3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26397
HGNC: HGNC:6843
Homologene: 56430
Hjurp
Name: Holliday junction recognition protein
Synonyms: 6430706D22Rik, C330011F01Rik, A730008H23Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381280
Homologene: 10184
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Palld
Name: palladin, cytoskeletal associated protein
Synonyms: 2410003B16Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 72333
Homologene: 75052
Kctd12
Name: potassium channel tetramerisation domain containing 12
Synonyms: Pfetin, Pfet1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 239217
Homologene: 16316
Washc5
Name: WASH complex subunit 5
Synonyms: strumpellin, E430025E21Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223593
VEGA: 15
Homologene: 8898
Bhlhe40
Name: basic helix-loop-helix family, member e40
Synonyms: cytokine response gene 8, Clast5, Stra13, Bhlhb2, C130042M06Rik, eip1 (E47 interaction protein 1), Stra14, Sharp2, DEC1, CR8
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20893
HGNC: HGNC:1046
Homologene: 2722
Srcap
Name: Snf2-related CREBBP activator protein
Synonyms: D030022P06Rik, F630004O05Rik, B930091H02Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043597
Homologene: 38213
Nek10
Name: NIMA (never in mitosis gene a)- related kinase 10
Synonyms: LOC238944
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 674895
Homologene: 130947
Gm4884
Name: predicted gene 4884
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233164
Neb
Name: nebulin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17996
HGNC: HGNC:7720
Homologene: 136285
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380698
Homologene: 70869
Prg4
Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)
Synonyms: MSF, DOL54, SZP, lubricin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 96875
HGNC: HGNC:9364
Homologene: 137352
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Itgad
Name: integrin, alpha D
Synonyms: Cd11d
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381924
HGNC: HGNC:6146
Homologene: 56919
Eif2b1
Name: eukaryotic translation initiation factor 2B, subunit alpha
Synonyms: EIF2BA, D5Ertd406e, EIF2B, 26kDa
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 209354
HGNC: HGNC:3257
Homologene: 1080
Megf8
Name: multiple EGF-like-domains 8
Synonyms: m687Ddg, b2b288Clo, b2b1702Clo, Egfl4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Fbrs
Name: fibrosin
Synonyms: Fbs1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14123
Homologene: 79554
Tbx18
Name: T-box18
Synonyms: 2810404D13Rik, 2810012F10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 76365
Homologene: 11384
Lipo3
Name: lipase, member O3
Synonyms: Lipo1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 381236
Homologene: 103863
Engase
Name: endo-beta-N-acetylglucosaminidase
Synonyms: D230014K01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217364
Homologene: 13666
AW551984
Name: expressed sequence AW551984
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244810
HGNC: HGNC:6658
Lgmn
Name: legumain
Synonyms: AEP, preprolegumain, Prsc1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19141
HGNC: HGNC:9472
Homologene: 38075
Tfpt
Name: TCF3 (E2A) fusion partner
Synonyms: 2400004F01Rik, Amida, FB1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69714
Homologene: 8349
Tmem132a
Name: transmembrane protein 132A
Synonyms: 6720481D13Rik, Hspa5bp1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 98170
Homologene: 75076
Plpbp
Name: pyridoxal phosphate binding protein
Synonyms: 2200002F22Rik, Prosc
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 114863
HGNC: HGNC:9457
Homologene: 5211
Ssr1
Name: signal sequence receptor, alpha
Synonyms: SSR, TRAPalpha
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107513
VEGA: 13
Homologene: 2368
Zfp24
Name: zinc finger protein 24
Synonyms: KOX17, Zfp191, 3526401F17Rik, 5033419P20Rik, ZF-12
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 59057
Homologene: 5068
Reep1
Name: receptor accessory protein 1
Synonyms: D6Ertd253e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 52250
Homologene: 41504
Kcnmb3
Name: potassium large conductance calcium-activated channel, subfamily M, beta member 3
Synonyms: Gm5707
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100502876
HGNC: HGNC:6287
Homologene: 18141
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • GT to GTT, chromosome 1 at 88,266,524 bp
  • C to A, chromosome 1 at 150,460,681 bp
  • T to A, chromosome 1 at 185,288,076 bp
  • A to T, chromosome 2 at 52,234,353 bp
  • A to G, chromosome 3 at 32,472,445 bp
  • T to C, chromosome 3 at 127,616,506 bp
  • A to T, chromosome 3 at 142,803,587 bp
  • T to A, chromosome 4 at 59,517,640 bp
  • A to G, chromosome 4 at 138,177,932 bp
  • C to T, chromosome 5 at 34,613,295 bp
  • A to G, chromosome 5 at 121,777,964 bp
  • G to A, chromosome 5 at 124,579,108 bp
  • T to A, chromosome 6 at 71,773,195 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • T to C, chromosome 7 at 3,620,836 bp
  • A to G, chromosome 7 at 19,532,123 bp
  • C to A, chromosome 7 at 25,358,734 bp
  • G to A, chromosome 7 at 41,044,622 bp
  • C to A, chromosome 7 at 89,307,163 bp
  • A to T, chromosome 7 at 89,307,164 bp
  • C to A, chromosome 7 at 127,487,919 bp
  • C to A, chromosome 7 at 127,542,391 bp
  • A to T, chromosome 7 at 128,185,948 bp
  • T to A, chromosome 8 at 27,052,279 bp
  • A to G, chromosome 8 at 61,533,443 bp
  • T to C, chromosome 9 at 39,589,099 bp
  • A to G, chromosome 9 at 87,707,811 bp
  • T to C, chromosome 10 at 76,217,374 bp
  • G to A, chromosome 11 at 59,039,009 bp
  • T to C, chromosome 11 at 60,942,324 bp
  • T to A, chromosome 11 at 118,481,316 bp
  • T to A, chromosome 12 at 102,402,692 bp
  • A to T, chromosome 13 at 37,987,690 bp
  • A to G, chromosome 13 at 55,669,724 bp
  • A to G, chromosome 14 at 14,861,684 bp
  • G to T, chromosome 14 at 102,982,186 bp
  • A to G, chromosome 15 at 59,340,890 bp
  • T to C, chromosome 16 at 31,131,315 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • G to A, chromosome 17 at 67,818,635 bp
  • T to C, chromosome 18 at 24,017,334 bp
  • C to A, chromosome 19 at 10,860,321 bp
  • A to G, chromosome 19 at 33,556,428 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6659 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044779-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.