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Strain Name:
C57BL/6J-MtgxR6724Btlr/Mmmh
Stock Number:
044842-MU
Citation ID:
RRID:MMRRC_044842-MU
Other Names:
R6724 (G1)
Major Collection:

Strain Information

Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467
Ppp2r3c
Name: protein phosphatase 2, regulatory subunit B'', gamma
Synonyms: 5730412A08Rik, G4-1, G5pr, 4930511A21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 59032
VEGA: 12
Homologene: 9915
Gcn1
Name: GCN1 activator of EIF2AK4
Synonyms: GCN1L, G431004K08Rik, Gcn1l1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231659
HGNC: HGNC:4199
Homologene: 5887
Ercc6
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
Synonyms: CS group B correcting gene, CSB, C130058G22Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319955
VEGA: 14
HGNC: HGNC:3438
Homologene: 133552
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 15,710,440 bp
  • G to A, chromosome 1 at 37,045,031 bp
  • C to T, chromosome 1 at 66,683,191 bp
  • T to C, chromosome 1 at 90,779,152 bp
  • C to T, chromosome 2 at 23,194,762 bp
  • C to A, chromosome 2 at 30,090,550 bp
  • T to A, chromosome 2 at 76,687,401 bp
  • A to T, chromosome 2 at 86,085,357 bp
  • T to G, chromosome 2 at 87,903,602 bp
  • T to G, chromosome 2 at 88,973,268 bp
  • A to T, chromosome 2 at 89,574,965 bp
  • T to C, chromosome 2 at 90,450,851 bp
  • A to C, chromosome 2 at 129,038,056 bp
  • G to A, chromosome 2 at 160,712,696 bp
  • T to A, chromosome 2 at 169,587,482 bp
  • T to C, chromosome 3 at 51,855,875 bp
  • T to A, chromosome 4 at 33,944,728 bp
  • A to G, chromosome 4 at 128,563,371 bp
  • G to A, chromosome 4 at 137,515,307 bp
  • C to A, chromosome 5 at 69,566,393 bp
  • T to A, chromosome 5 at 70,754,209 bp
  • T to G, chromosome 5 at 74,591,774 bp
  • T to G, chromosome 5 at 115,609,158 bp
  • T to A, chromosome 6 at 18,255,974 bp
  • A to G, chromosome 6 at 30,446,641 bp
  • T to C, chromosome 6 at 52,215,739 bp
  • T to A, chromosome 6 at 53,819,574 bp
  • A to T, chromosome 6 at 116,414,548 bp
  • T to A, chromosome 6 at 129,906,680 bp
  • T to C, chromosome 6 at 134,486,541 bp
  • A to T, chromosome 6 at 148,809,975 bp
  • A to T, chromosome 7 at 12,260,672 bp
  • T to C, chromosome 7 at 19,918,679 bp
  • A to G, chromosome 7 at 47,235,038 bp
  • A to T, chromosome 7 at 86,954,258 bp
  • A to G, chromosome 7 at 130,728,762 bp
  • G to A, chromosome 7 at 145,256,520 bp
  • A to T, chromosome 8 at 13,837,083 bp
  • G to A, chromosome 8 at 27,114,182 bp
  • A to G, chromosome 9 at 55,541,288 bp
  • G to A, chromosome 9 at 108,574,894 bp
  • C to G, chromosome 9 at 111,365,916 bp
  • A to G, chromosome 10 at 59,892,780 bp
  • T to C, chromosome 11 at 23,729,037 bp
  • A to G, chromosome 11 at 76,971,907 bp
  • A to T, chromosome 11 at 97,206,002 bp
  • T to A, chromosome 12 at 3,686,817 bp
  • A to T, chromosome 12 at 55,288,496 bp
  • A to T, chromosome 12 at 87,621,984 bp
  • C to T, chromosome 12 at 103,104,861 bp
  • G to T, chromosome 12 at 103,860,420 bp
  • A to T, chromosome 13 at 92,575,484 bp
  • A to T, chromosome 14 at 26,796,223 bp
  • A to T, chromosome 14 at 32,566,331 bp
  • A to G, chromosome 14 at 75,052,010 bp
  • A to G, chromosome 14 at 123,298,067 bp
  • C to T, chromosome 15 at 57,247,532 bp
  • G to A, chromosome 15 at 89,532,453 bp
  • A to T, chromosome 16 at 59,544,138 bp
  • T to A, chromosome 16 at 85,868,557 bp
  • G to A, chromosome 17 at 30,748,568 bp
  • T to A, chromosome 17 at 33,886,733 bp
  • A to G, chromosome 17 at 64,731,269 bp
  • A to G, chromosome 19 at 7,802,361 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6724 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044842-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.