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Strain Name:
C57BL/6J-MtgxR6735Btlr/Mmmh
Stock Number:
044853-MU
Citation ID:
RRID:MMRRC_044853-MU
Other Names:
R6735 (G1)
Major Collection:

Strain Information

Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Nts
Name: neurotensin
Synonyms: neuromedin N, NT/N, NTS1, NMN-125, 5033428E16Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67405
VEGA: 10
HGNC: HGNC:8038
Homologene: 4506
Thbs4
Name: thrombospondin 4
Synonyms: TSP-4, TSP4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21828
Homologene: 20691
Cnot2
Name: CCR4-NOT transcription complex, subunit 2
Synonyms: 2600016M12Rik, 2810470K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72068
HGNC: HGNC:7878
Homologene: 40953
Erbin
Name: Erbb2 interacting protein
Synonyms: 1700028E05Rik, Erbin, Erbb2ip
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 59079
Homologene: 41282
Usp15
Name: ubiquitin specific peptidase 15
Synonyms: Gcap18, 4921514G19Rik, E430033I05Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14479
VEGA: 10
Homologene: 101542
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 21,229,581 bp
  • T to G, chromosome 2 at 36,091,378 bp
  • C to T, chromosome 2 at 45,110,016 bp
  • C to A, chromosome 2 at 76,798,908 bp
  • T to C, chromosome 2 at 83,923,761 bp
  • A to C, chromosome 2 at 85,975,434 bp
  • T to A, chromosome 3 at 98,134,586 bp
  • A to G, chromosome 3 at 127,724,449 bp
  • A to G, chromosome 4 at 147,941,817 bp
  • A to T, chromosome 5 at 38,201,058 bp
  • C to A, chromosome 5 at 86,309,300 bp
  • A to G, chromosome 5 at 100,562,619 bp
  • A to G, chromosome 5 at 104,480,329 bp
  • A to T, chromosome 5 at 139,293,145 bp
  • T to C, chromosome 6 at 43,275,032 bp
  • T to C, chromosome 6 at 145,160,893 bp
  • T to C, chromosome 7 at 10,447,211 bp
  • A to T, chromosome 7 at 13,665,058 bp
  • T to C, chromosome 7 at 24,261,772 bp
  • T to C, chromosome 7 at 97,382,982 bp
  • A to G, chromosome 7 at 121,742,263 bp
  • A to G, chromosome 8 at 68,120,746 bp
  • A to G, chromosome 9 at 25,303,752 bp
  • A to G, chromosome 10 at 24,807,453 bp
  • A to G, chromosome 10 at 61,741,267 bp
  • A to T, chromosome 10 at 94,074,320 bp
  • T to A, chromosome 10 at 102,484,998 bp
  • G to A, chromosome 10 at 116,498,153 bp
  • T to C, chromosome 10 at 123,168,367 bp
  • T to A, chromosome 11 at 31,924,700 bp
  • T to A, chromosome 12 at 83,594,844 bp
  • A to G, chromosome 13 at 46,752,746 bp
  • T to C, chromosome 13 at 59,625,564 bp
  • T to A, chromosome 13 at 92,755,166 bp
  • A to T, chromosome 13 at 103,884,210 bp
  • A to T, chromosome 15 at 6,489,906 bp
  • G to A, chromosome 15 at 75,756,634 bp
  • A to T, chromosome 15 at 98,909,240 bp
  • C to T, chromosome 15 at 99,314,903 bp
  • T to A, chromosome 16 at 8,855,900 bp
  • A to G, chromosome 16 at 31,945,543 bp
  • A to G, chromosome 17 at 56,585,169 bp
  • T to G, chromosome 17 at 88,486,795 bp
  • T to C, chromosome 18 at 32,123,648 bp
  • T to C, chromosome 18 at 42,688,588 bp
  • A to G, chromosome 19 at 8,736,222 bp
  • C to A, chromosome X at 8,062,899 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6735 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044853-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.