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Strain Name:
C57BL/6J-MtgxR6812Btlr/Mmmh
Stock Number:
044924-MU
Citation ID:
RRID:MMRRC_044924-MU
Other Names:
R6812 (G1)
Major Collection:

Strain Information

Pald1
Name: phosphatase domain containing, paladin 1
Synonyms: paladin, X99384
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 27355
VEGA: 10
Homologene: 8453
Lias
Name: lipoic acid synthetase
Synonyms: MGC7254, mLip1, 4933425M12Rik, 2900022L22Rik, 7a5ex
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 79464
Homologene: 4997
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Kif2a
Name: kinesin family member 2A
Synonyms: Kns2, Kif2, M-kinesin
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16563
HGNC: HGNC:6318
Homologene: 3320
Gk5
Name: glycerol kinase 5
Synonyms: G630067D24Rik, C330018K18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235533
Homologene: 15141
Dcaf1
Name: DDB1 and CUL4 associated factor 1
Synonyms: B930007L02Rik, Vprbp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 321006
Homologene: 8805
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: eIF4GII, 1500002J22Rik, 4930523M17Rik, G1-419-52, repro8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 172,284,877 bp
  • T to A, chromosome 2 at 32,213,141 bp
  • C to T, chromosome 2 at 112,946,906 bp
  • C to A, chromosome 3 at 89,094,476 bp
  • T to A, chromosome 4 at 105,047,152 bp
  • A to G, chromosome 4 at 118,455,264 bp
  • G to T, chromosome 4 at 138,103,376 bp
  • A to G, chromosome 5 at 3,596,105 bp
  • T to A, chromosome 5 at 65,408,789 bp
  • A to G, chromosome 6 at 43,081,788 bp
  • A to G, chromosome 6 at 125,337,856 bp
  • A to G, chromosome 6 at 136,697,995 bp
  • C to T, chromosome 6 at 144,116,443 bp
  • T to C, chromosome 6 at 149,081,132 bp
  • T to C, chromosome 6 at 149,409,537 bp
  • T to C, chromosome 7 at 7,206,308 bp
  • A to T, chromosome 7 at 25,713,044 bp
  • G to T, chromosome 7 at 30,634,001 bp
  • G to T, chromosome 7 at 81,535,089 bp
  • T to A, chromosome 7 at 98,888,482 bp
  • T to A, chromosome 8 at 128,705,410 bp
  • T to G, chromosome 9 at 7,854,417 bp
  • C to T, chromosome 9 at 46,210,769 bp
  • T to C, chromosome 9 at 96,150,749 bp
  • T to C, chromosome 9 at 106,858,069 bp
  • A to G, chromosome 9 at 110,987,112 bp
  • G to T, chromosome 10 at 39,823,548 bp
  • A to T, chromosome 10 at 41,367,167 bp
  • A to G, chromosome 10 at 61,342,922 bp
  • A to G, chromosome 10 at 62,959,341 bp
  • A to G, chromosome 10 at 105,413,269 bp
  • T to A, chromosome 10 at 116,832,913 bp
  • C to T, chromosome 11 at 65,981,329 bp
  • T to A, chromosome 11 at 67,086,402 bp
  • T to C, chromosome 11 at 70,000,995 bp
  • A to T, chromosome 12 at 7,983,062 bp
  • A to G, chromosome 12 at 71,047,263 bp
  • C to A, chromosome 12 at 100,878,411 bp
  • T to C, chromosome 13 at 55,421,845 bp
  • A to T, chromosome 13 at 94,479,861 bp
  • A to T, chromosome 13 at 106,969,751 bp
  • T to A, chromosome 14 at 79,197,419 bp
  • A to G, chromosome 14 at 104,510,432 bp
  • G to T, chromosome 15 at 101,997,979 bp
  • C to T, chromosome 16 at 56,795,798 bp
  • G to A, chromosome 17 at 44,085,576 bp
  • G to GAAGAAT, chromosome 17 at 46,412,542 bp
  • T to A, chromosome 17 at 46,661,409 bp
  • T to A, chromosome 17 at 78,315,600 bp
  • T to A, chromosome 18 at 42,333,403 bp
  • T to C, chromosome 19 at 10,622,499 bp
  • T to C, chromosome 19 at 13,479,611 bp
  • A to C, chromosome 19 at 20,900,861 bp
  • T to C, chromosome 19 at 56,351,676 bp
  • G to A, chromosome X at 160,533,048 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6812 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044924-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.