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Strain Name:
C57BL/6J-MtgxR6815Btlr/Mmmh
Stock Number:
044927-MU
Citation ID:
RRID:MMRRC_044927-MU
Other Names:
R6815 (G1)
Major Collection:

Strain Information

Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Dnajc21
Name: DnaJ heat shock protein family (Hsp40) member C21
Synonyms: 9930116P15Rik, 4930461P20Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78244
Homologene: 6752
Prkd2
Name: protein kinase D2
Synonyms: PKD2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101540
Homologene: 9516
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, Aspm, D330028K02Rik, MCPH5, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Heatr5a
Name: HEAT repeat containing 5A
Synonyms: D930036F22Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320487
VEGA: 12
Homologene: 19635
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 34,228,369 bp
  • C to A, chromosome 1 at 74,679,349 bp
  • C to T, chromosome 1 at 80,538,859 bp
  • A to G, chromosome 1 at 139,480,142 bp
  • T to C, chromosome 2 at 35,149,491 bp
  • T to C, chromosome 2 at 66,226,790 bp
  • T to A, chromosome 2 at 101,630,555 bp
  • A to C, chromosome 2 at 132,809,858 bp
  • A to G, chromosome 2 at 152,441,055 bp
  • A to G, chromosome 3 at 122,824,924 bp
  • A to C, chromosome 3 at 131,298,010 bp
  • T to A, chromosome 4 at 113,717,127 bp
  • T to A, chromosome 4 at 117,229,937 bp
  • G to T, chromosome 5 at 31,134,080 bp
  • T to A, chromosome 5 at 92,612,486 bp
  • T to C, chromosome 5 at 129,692,987 bp
  • A to T, chromosome 6 at 34,412,334 bp
  • G to T, chromosome 6 at 38,818,842 bp
  • A to G, chromosome 6 at 94,625,029 bp
  • A to G, chromosome 6 at 120,521,209 bp
  • G to A, chromosome 6 at 129,516,742 bp
  • T to C, chromosome 7 at 16,843,793 bp
  • T to A, chromosome 7 at 19,222,635 bp
  • T to A, chromosome 7 at 35,749,847 bp
  • C to T, chromosome 7 at 80,766,884 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • C to T, chromosome 7 at 119,971,727 bp
  • A to G, chromosome 8 at 45,941,987 bp
  • A to T, chromosome 8 at 47,193,101 bp
  • A to T, chromosome 8 at 117,113,522 bp
  • G to T, chromosome 9 at 19,477,765 bp
  • T to A, chromosome 9 at 57,117,540 bp
  • A to G, chromosome 9 at 104,081,327 bp
  • A to G, chromosome 10 at 128,990,322 bp
  • A to C, chromosome 11 at 69,232,001 bp
  • A to T, chromosome 11 at 95,749,404 bp
  • T to C, chromosome 11 at 96,934,607 bp
  • C to T, chromosome 11 at 105,997,258 bp
  • T to G, chromosome 12 at 51,955,508 bp
  • T to A, chromosome 12 at 71,017,082 bp
  • T to A, chromosome 12 at 83,979,727 bp
  • C to A, chromosome 12 at 109,594,503 bp
  • A to T, chromosome 13 at 95,018,174 bp
  • C to T, chromosome 13 at 102,705,429 bp
  • T to G, chromosome 14 at 54,591,153 bp
  • A to G, chromosome 14 at 113,314,860 bp
  • C to T, chromosome 15 at 10,447,691 bp
  • A to G, chromosome 15 at 44,562,655 bp
  • T to C, chromosome 16 at 13,123,435 bp
  • T to C, chromosome 16 at 20,333,630 bp
  • A to G, chromosome 16 at 39,008,881 bp
  • A to G, chromosome 18 at 36,975,121 bp
  • A to T, chromosome 19 at 4,116,066 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6815 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044927-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.