Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6816Btlr/Mmmh
Stock Number:
044928-MU
Citation ID:
RRID:MMRRC_044928-MU
Other Names:
R6816 (G1)
Major Collection:

Strain Information

Sulf2
Name: sulfatase 2
Synonyms: 2010004N24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 72043
Homologene: 10313
Hgs
Name: HGF-regulated tyrosine kinase substrate
Synonyms: Hrs, tn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15239
HGNC: HGNC:4897
Homologene: 37954
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Sema3b
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Synonyms: SemA, semaV, sema5, SemA, LUCA-1, Semaa
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20347
Homologene: 20972
Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194
Madcam1
Name: mucosal vascular addressin cell adhesion molecule 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17123
VEGA: 10
HGNC: HGNC:6765
Homologene: 8413
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 36,406,510 bp
  • A to T, chromosome 1 at 84,793,714 bp
  • C to A, chromosome 1 at 119,477,982 bp
  • T to A, chromosome 1 at 134,745,800 bp
  • T to A, chromosome 1 at 135,959,728 bp
  • A to G, chromosome 2 at 10,105,706 bp
  • T to C, chromosome 2 at 25,181,307 bp
  • C to CTCTA, chromosome 2 at 25,579,721 bp
  • T to C, chromosome 2 at 125,595,027 bp
  • G to T, chromosome 2 at 158,761,675 bp
  • G to T, chromosome 2 at 164,501,132 bp
  • T to C, chromosome 2 at 166,082,754 bp
  • T to A, chromosome 3 at 61,003,832 bp
  • A to G, chromosome 3 at 96,854,570 bp
  • T to C, chromosome 3 at 100,191,705 bp
  • A to T, chromosome 3 at 104,089,911 bp
  • G to A, chromosome 4 at 63,611,955 bp
  • A to C, chromosome 5 at 17,670,465 bp
  • T to C, chromosome 5 at 25,405,532 bp
  • A to T, chromosome 5 at 94,683,914 bp
  • T to A, chromosome 5 at 135,159,215 bp
  • C to T, chromosome 6 at 115,611,728 bp
  • A to C, chromosome 7 at 29,311,662 bp
  • A to G, chromosome 7 at 44,016,868 bp
  • A to G, chromosome 7 at 45,733,678 bp
  • A to G, chromosome 7 at 45,833,682 bp
  • A to T, chromosome 7 at 48,875,583 bp
  • T to A, chromosome 7 at 85,961,413 bp
  • G to T, chromosome 7 at 100,629,873 bp
  • T to C, chromosome 7 at 132,634,033 bp
  • C to T, chromosome 8 at 25,937,734 bp
  • A to G, chromosome 8 at 94,941,683 bp
  • G to T, chromosome 8 at 105,330,544 bp
  • T to A, chromosome 8 at 123,359,413 bp
  • A to G, chromosome 9 at 40,211,813 bp
  • C to T, chromosome 9 at 63,480,759 bp
  • T to A, chromosome 9 at 107,600,350 bp
  • T to C, chromosome 9 at 107,900,410 bp
  • A to T, chromosome 10 at 24,092,181 bp
  • A to G, chromosome 10 at 79,665,440 bp
  • T to G, chromosome 11 at 5,643,765 bp
  • T to C, chromosome 11 at 87,765,665 bp
  • T to G, chromosome 11 at 100,911,267 bp
  • T to A, chromosome 11 at 120,471,571 bp
  • G to A, chromosome 12 at 9,027,724 bp
  • A to G, chromosome 12 at 81,722,254 bp
  • C to T, chromosome 13 at 21,787,777 bp
  • A to T, chromosome 16 at 97,568,467 bp
  • T to C, chromosome 17 at 12,652,483 bp
  • A to T, chromosome 17 at 12,714,082 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6816 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044928-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.