Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6849Btlr/Mmmh
Stock Number:
044953-MU
Citation ID:
RRID:MMRRC_044953-MU
Other Names:
R6849 (G1)
Major Collection:

Strain Information

Apoa5
Name: apolipoprotein A-V
Synonyms: RAP3, Apoav, 1300007O05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66113
Homologene: 14197
Itk
Name: IL2 inducible T cell kinase
Synonyms: Emt, Tsk, Tcsk
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16428
HGNC: HGNC:6171
Homologene: 4051
Csnk2a1
Name: casein kinase 2, alpha 1 polypeptide
Synonyms: Csnk2a1-rs4, CK2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12995
Homologene: 90874
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, seal, motor end-plate disease, nmf2, ataxia 3, mnd2, mnd-2, C630029C19Rik, nmf58, NaCh6, Nav1.6, nmf335, NMF335, nur14
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20273
Homologene: 7927
Pi4ka
Name: phosphatidylinositol 4-kinase alpha
Synonyms: Pik4ca
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224020
HGNC: HGNC:8983
Homologene: 11171
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: c-Cbl-associated protein, CAP, Sh3d5, 9530001P15Rik, 2310065E01Rik, Ponsin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Ubr7
Name: ubiquitin protein ligase E3 component n-recognin 7 (putative)
Synonyms: 5730410I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 66622
VEGA: 12
Homologene: 11998
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 91,254,213 bp
  • T to A, chromosome 1 at 131,977,964 bp
  • G to A, chromosome 1 at 136,092,694 bp
  • A to T, chromosome 1 at 181,808,945 bp
  • T to A, chromosome 2 at 76,914,343 bp
  • T to C, chromosome 2 at 125,321,691 bp
  • C to A, chromosome 2 at 152,250,564 bp
  • T to C, chromosome 3 at 109,521,466 bp
  • T to C, chromosome 3 at 129,829,389 bp
  • A to T, chromosome 4 at 152,378,538 bp
  • C to A, chromosome 5 at 76,857,010 bp
  • C to T, chromosome 5 at 76,857,157 bp
  • T to A, chromosome 5 at 86,496,632 bp
  • T to G, chromosome 6 at 113,302,118 bp
  • A to G, chromosome 7 at 5,480,807 bp
  • C to T, chromosome 7 at 72,211,718 bp
  • T to A, chromosome 7 at 83,586,357 bp
  • T to C, chromosome 7 at 86,307,040 bp
  • T to A, chromosome 7 at 100,406,927 bp
  • T to A, chromosome 8 at 69,302,651 bp
  • G to T, chromosome 8 at 124,639,522 bp
  • A to G, chromosome 8 at 125,861,210 bp
  • A to G, chromosome 9 at 44,387,264 bp
  • A to G, chromosome 9 at 46,270,000 bp
  • C to T, chromosome 9 at 51,138,714 bp
  • A to G, chromosome 9 at 56,619,616 bp
  • A to G, chromosome 9 at 70,818,847 bp
  • A to C, chromosome 9 at 75,009,312 bp
  • G to T, chromosome 9 at 108,975,053 bp
  • A to T, chromosome 9 at 114,631,936 bp
  • A to T, chromosome 10 at 77,770,910 bp
  • T to C, chromosome 11 at 46,331,935 bp
  • A to G, chromosome 11 at 66,525,501 bp
  • T to A, chromosome 11 at 74,085,450 bp
  • A to G, chromosome 12 at 84,443,787 bp
  • T to C, chromosome 12 at 102,758,083 bp
  • T to A, chromosome 13 at 34,050,269 bp
  • T to C, chromosome 13 at 49,067,358 bp
  • T to C, chromosome 13 at 95,047,799 bp
  • A to T, chromosome 14 at 63,986,238 bp
  • G to T, chromosome 14 at 63,986,239 bp
  • A to G, chromosome 15 at 28,278,624 bp
  • A to G, chromosome 15 at 35,905,309 bp
  • T to A, chromosome 15 at 82,079,945 bp
  • T to A, chromosome 15 at 92,305,246 bp
  • T to A, chromosome 15 at 100,955,587 bp
  • G to A, chromosome 16 at 17,303,421 bp
  • A to G, chromosome 16 at 20,680,745 bp
  • A to G, chromosome 16 at 37,821,159 bp
  • T to G, chromosome 17 at 18,443,919 bp
  • G to T, chromosome 17 at 18,443,920 bp
  • T to C, chromosome 17 at 36,189,805 bp
  • A to C, chromosome 17 at 80,630,413 bp
  • A to G, chromosome 18 at 67,812,435 bp
  • G to T, chromosome 19 at 13,838,839 bp
  • G to C, chromosome 19 at 40,376,800 bp
  • A to G, chromosome 19 at 46,317,746 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6849 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044953-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.