Strain Name:
C57BL/6J-MtgxR6854Btlr/Mmmh
Stock Number:
044957-MU
Citation ID:
RRID:MMRRC_044957-MU
Other Names:
R6854 (G1)
Major Collection:

Strain Information

Cdc25a
Name: cell division cycle 25A
Synonyms: D9Ertd393e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12530
HGNC: HGNC:1725
Homologene: 1355
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Pml
Name: promyelocytic leukemia
Synonyms: Trim19, 1200009E24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18854
HGNC: HGNC:9113
Homologene: 13245
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, Aspm, D330028K02Rik, MCPH5, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Zfp318
Name: zinc finger protein 318
Synonyms: TZF, 2610034E08Rik, D530032D06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 57908
Homologene: 22808
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 404710
Homologene: 26400
Ccnd3
Name: cyclin D3
Synonyms: 9230106B05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12445
HGNC: HGNC:1585
Homologene: 20419
Ppp6r1
Name: protein phosphatase 6, regulatory subunit 1
Synonyms: 2010309P17Rik, B430201G11Rik, Pp6r1, Saps1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243819
Homologene: 86982
St3gal3
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 3
Synonyms: ST3Gal III, Siat3, Siat6
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 20441
Homologene: 7539
Klrb1c
Name: killer cell lectin-like receptor subfamily B member 1C
Synonyms: Nkrp1-c, NK-RP1, Nk1.1, CD161, NK-1.1, Ly-59, Nk-1, Ly55c, Nk-1.2, NKR-P1, Nk1, Ly59, NKR-P1C
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17059
HGNC: HGNC:6373
Homologene: 84369
Sptb
Name: spectrin beta, erythrocytic
Synonyms: Spnb-1, spectrin R, D330027P03Rik, LOC383567, brain erythroid spectrin (235E), Spnb1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20741
Homologene: 295
Flrt3
Name: fibronectin leucine rich transmembrane protein 3
Synonyms: 5530600M07Rik, C430047I10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71436
HGNC: HGNC:3762
Homologene: 8322
Itsn2
Name: intersectin 2
Synonyms: Sh3p18, Ese2, Eh domain, SH3 domain regulator of endocytosis 2, Sh3d1B
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20403
VEGA: 12
HGNC: HGNC:6184
Homologene: 22627
Bicra
Name: BRD4 interacting chromatin remodeling complex associated protein
Synonyms: Gltscr1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243842
HGNC: HGNC:4332
Homologene: 9250
Serinc4
Name: serine incorporator 4
Synonyms: A930015D22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 574418
Homologene: 67424
Siglecf
Name: sialic acid binding Ig-like lectin F
Synonyms: mSiglec-F, Siglec5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233186
Homologene: 50482
Atp4a
Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
Synonyms: H+K+-transporting alpha 1, H+/K+-ATPase alpha
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11944
HGNC: HGNC:819
Homologene: 68081
Maml2
Name: mastermind like transcriptional coactivator 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270118
Homologene: 134147
Nlrp1b
Name: NLR family, pyrin domain containing 1B
Synonyms: Nalp1b
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 637515
Homologene: 19080
Pus7
Name: pseudouridylate synthase 7
Synonyms: C330017I15Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 78697
Homologene: 6998
Cfap44
Name: cilia and flagella associated protein 44
Synonyms: 6330444M21Rik, D16Ertd642e, Wdr52
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 212517
Homologene: 75085
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Sema4f
Name: sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain
Synonyms: Sema W
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20355
Homologene: 3147
Speer2
Name: spermatogenesis associated glutamate (E)-rich protein 2
Synonyms: SPEER-2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224318
Homologene: 69402
Ahnak
Name: AHNAK nucleoprotein
Synonyms: DY6, 2310047C17Rik, 1110004P15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Hivep1
Name: human immunodeficiency virus type I enhancer binding protein 1
Synonyms: Cryabp1, alphaA-CRYBP1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110521
VEGA: 13
HGNC: HGNC:4920
Homologene: 1596
Chd5
Name: chromodomain helicase DNA binding protein 5
Synonyms: B230399N07Rik, 4930532L22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269610
Homologene: 56712
Vsig10
Name: V-set and immunoglobulin domain containing 10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231668
Homologene: 10414
Palmd
Name: palmdelphin
Synonyms: PALML, 4631423C22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 114301
Homologene: 9804
Or5h19
Name: olfactory receptor family 5 subfamily H member 19
Synonyms: GA_x54KRFPKG5P-55265713-55264787, MOR183-8, Olfr187
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258319
Homologene: 133069
Phyhd1
Name: phytanoyl-CoA dioxygenase domain containing 1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227696
Homologene: 14939
Rdh7
Name: retinol dehydrogenase 7
Synonyms: CRAD2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 54150
Homologene: 137324
Catsperg1
Name: cation channel sperm associated auxiliary subunit gamma 1
Synonyms: A230107C01Rik, Catsperg
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 320225
Homologene: 10915
Plcd1
Name: phospholipase C, delta 1
Synonyms: PLC-delta 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 18799
VEGA: 9
HGNC: HGNC:9060
Homologene: 21252
Ttll3
Name: tubulin tyrosine ligase-like family, member 3
Synonyms: 4833441J24Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101100
Homologene: 134361
Repin1
Name: replication initiator 1
Synonyms: AP4, E430037F08Rik, Zfp464
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58887
Homologene: 22810
Slc66a3
Name: solute carrier family 66 member 3
Synonyms: E030024M05Rik, Pqlc3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217430
Homologene: 16454
Tmem25
Name: transmembrane protein 25
Synonyms: 0610039J01Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71687
Homologene: 12403
Mycl
Name: v-myc avian myelocytomatosis viral oncogene lung carcinoma derived
Synonyms: Lmyc-1, Lmyc1, L-myc, bHLHe38, Mycl1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16918
Homologene: 3921
Prr23a1
Name: proline rich 23A, member 1
Synonyms: Gm6400, Prr23a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 623166
Homologene: 67036
BC035947
Name: cDNA sequence BC035947
Type: Gene
Species: Mouse
Chromosome: 1
Or4f14c
Name: olfactory receptor family 4 subfamily F member 14C
Synonyms: GA_x6K02T2Q125-73157028-73156524, MOR245-1, Olfr1315
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 404497
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 78,498,488 bp
  • G to A, chromosome 1 at 88,243,950 bp
  • G to A, chromosome 1 at 139,463,182 bp
  • A to C, chromosome 2 at 30,269,761 bp
  • T to C, chromosome 2 at 112,110,647 bp
  • T to A, chromosome 2 at 121,456,550 bp
  • C to T, chromosome 2 at 140,660,718 bp
  • T to C, chromosome 3 at 88,096,951 bp
  • A to G, chromosome 3 at 93,398,123 bp
  • A to G, chromosome 3 at 116,923,463 bp
  • C to A, chromosome 4 at 117,958,530 bp
  • G to A, chromosome 4 at 123,000,246 bp
  • C to A, chromosome 4 at 152,382,938 bp
  • T to C, chromosome 5 at 23,768,847 bp
  • G to A, chromosome 5 at 117,338,407 bp
  • G to T, chromosome 6 at 48,593,891 bp
  • A to G, chromosome 6 at 82,918,002 bp
  • CAAAGTAA to CAAAGTAAAGTAA, chromosome 6 at 113,399,157 bp
  • A to G, chromosome 6 at 128,788,418 bp
  • C to A, chromosome 7 at 4,632,396 bp
  • A to T, chromosome 7 at 15,988,762 bp
  • A to T, chromosome 7 at 29,181,702 bp
  • T to C, chromosome 7 at 30,715,008 bp
  • T to C, chromosome 7 at 43,352,180 bp
  • C to T, chromosome 9 at 13,705,835 bp
  • T to C, chromosome 9 at 44,796,008 bp
  • G to C, chromosome 9 at 58,219,906 bp
  • G to T, chromosome 9 at 98,842,935 bp
  • A to G, chromosome 9 at 109,879,927 bp
  • T to C, chromosome 9 at 119,074,321 bp
  • T to C, chromosome 10 at 127,888,381 bp
  • G to A, chromosome 11 at 71,228,433 bp
  • A to G, chromosome 12 at 4,652,382 bp
  • G to A, chromosome 12 at 16,999,829 bp
  • G to A, chromosome 12 at 76,603,480 bp
  • A to G, chromosome 13 at 42,156,507 bp
  • C to T, chromosome 16 at 15,651,538 bp
  • A to G, chromosome 16 at 44,449,028 bp
  • A to C, chromosome 16 at 59,036,065 bp
  • T to A, chromosome 16 at 69,858,887 bp
  • T to A, chromosome 17 at 36,057,218 bp
  • G to GAAGAAA, chromosome 17 at 46,412,542 bp
  • T to C, chromosome 17 at 47,578,720 bp
  • T to C, chromosome 19 at 9,015,235 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6854 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044957-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.