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Strain Name:
C57BL/6J-MtgxR6888Btlr/Mmmh
Stock Number:
044982-MU
Citation ID:
RRID:MMRRC_044982-MU
Other Names:
R6888 (G1)
Major Collection:

Strain Information

Cdh1
Name: cadherin 1
Synonyms: E-cadherin, Ecad, UM, uvomorulin, L-CAM, E-cad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12550
HGNC: HGNC:1748
Homologene: 20917
Limch1
Name: LIM and calponin homology domains 1
Synonyms: 3732412D22Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77569
Homologene: 18953
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Secisbp2
Name: SECIS binding protein 2
Synonyms: 2210413N07Rik, SBP2, 2810012K13Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75420
Homologene: 11415
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 52184
Homologene: 12011
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 135,982,480 bp
  • A to T, chromosome 1 at 153,262,492 bp
  • G to A, chromosome 2 at 26,289,734 bp
  • A to G, chromosome 2 at 41,471,126 bp
  • A to T, chromosome 2 at 69,524,141 bp
  • G to A, chromosome 2 at 76,761,103 bp
  • A to G, chromosome 2 at 88,050,264 bp
  • A to G, chromosome 2 at 91,421,894 bp
  • A to G, chromosome 2 at 91,769,027 bp
  • A to G, chromosome 2 at 125,257,124 bp
  • A to G, chromosome 2 at 126,904,992 bp
  • A to G, chromosome 2 at 143,826,719 bp
  • A to T, chromosome 2 at 167,038,940 bp
  • T to C, chromosome 3 at 145,148,618 bp
  • A to G, chromosome 4 at 101,890,256 bp
  • T to C, chromosome 4 at 140,034,743 bp
  • A to G, chromosome 4 at 141,776,992 bp
  • T to C, chromosome 5 at 24,383,335 bp
  • C to T, chromosome 5 at 67,021,926 bp
  • T to C, chromosome 5 at 120,480,900 bp
  • T to C, chromosome 5 at 123,811,310 bp
  • G to A, chromosome 6 at 18,313,730 bp
  • C to A, chromosome 6 at 38,952,074 bp
  • T to G, chromosome 6 at 50,180,277 bp
  • G to T, chromosome 6 at 111,358,353 bp
  • T to C, chromosome 6 at 119,948,781 bp
  • G to A, chromosome 6 at 137,380,200 bp
  • A to T, chromosome 7 at 22,872,106 bp
  • G to A, chromosome 7 at 27,519,634 bp
  • T to C, chromosome 7 at 100,930,820 bp
  • A to G, chromosome 7 at 109,029,298 bp
  • A to T, chromosome 8 at 20,944,555 bp
  • G to A, chromosome 8 at 36,993,833 bp
  • G to T, chromosome 8 at 71,603,739 bp
  • G to A, chromosome 8 at 71,914,286 bp
  • A to G, chromosome 8 at 83,271,588 bp
  • A to G, chromosome 8 at 88,170,884 bp
  • A to G, chromosome 8 at 106,658,314 bp
  • A to G, chromosome 8 at 111,451,043 bp
  • C to T, chromosome 9 at 103,499,716 bp
  • C to A, chromosome 9 at 113,671,837 bp
  • T to A, chromosome 9 at 114,404,701 bp
  • T to C, chromosome 10 at 4,546,854 bp
  • A to G, chromosome 10 at 4,857,076 bp
  • A to G, chromosome 10 at 89,456,542 bp
  • A to T, chromosome 11 at 49,460,260 bp
  • T to C, chromosome 11 at 51,788,192 bp
  • T to A, chromosome 11 at 94,459,207 bp
  • T to C, chromosome 11 at 96,916,192 bp
  • T to C, chromosome 11 at 120,347,315 bp
  • A to G, chromosome 13 at 51,679,941 bp
  • A to G, chromosome 13 at 81,508,669 bp
  • A to T, chromosome 13 at 94,408,791 bp
  • A to G, chromosome 13 at 98,637,061 bp
  • T to C, chromosome 15 at 31,459,233 bp
  • T to C, chromosome 15 at 66,696,246 bp
  • T to C, chromosome 15 at 74,613,249 bp
  • A to T, chromosome 16 at 43,817,139 bp
  • T to C, chromosome 17 at 33,963,206 bp
  • A to G, chromosome 17 at 34,594,107 bp
  • A to G, chromosome 17 at 57,102,143 bp
  • C to T, chromosome 19 at 7,457,249 bp
  • A to T, chromosome 19 at 48,693,824 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6888 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044982-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.