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Strain Name:
C57BL/6J-MtgxR6921Btlr/Mmmh
Stock Number:
045006-MU
Citation ID:
RRID:MMRRC_045006-MU
Other Names:
R6921 (G1)
Major Collection:

Strain Information

Fam117b
Name: family with sequence similarity 117, member B
Synonyms: 6330416D14Rik, 2810425F24Rik, Als2cr13
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72750
Homologene: 18307
Ip6k1
Name: inositol hexaphosphate kinase 1
Synonyms: InsP6, 1200016D08Rik, Ihpk1, InsP6k1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 27399
Homologene: 56602
Adgrl3
Name: adhesion G protein-coupled receptor L3
Synonyms: lectomedin 3, LEC3, D130075K09Rik, 5430402I23Rik, Lphn3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 319387
Homologene: 22878
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, A230102L03Rik, Atbf1, Sci
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Dis3l2
Name: DIS3 like 3'-5' exoribonuclease 2
Synonyms: 8030493P09Rik, 4930429A22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 208718
Homologene: 62417
Dmtf1
Name: cyclin D binding myb like transcription factor 1
Synonyms: Dmp1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 23857
Homologene: 8017
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 43,051,896 bp
  • A to G, chromosome 1 at 59,952,935 bp
  • T to C, chromosome 1 at 86,857,341 bp
  • T to A, chromosome 1 at 91,370,332 bp
  • A to T, chromosome 1 at 172,111,924 bp
  • G to A, chromosome 2 at 5,000,935 bp
  • T to C, chromosome 2 at 74,728,492 bp
  • C to T, chromosome 2 at 86,159,852 bp
  • C to T, chromosome 3 at 63,707,734 bp
  • A to G, chromosome 3 at 70,028,100 bp
  • A to T, chromosome 4 at 45,044,849 bp
  • C to T, chromosome 4 at 48,051,486 bp
  • A to G, chromosome 4 at 48,396,260 bp
  • T to A, chromosome 4 at 132,544,720 bp
  • T to C, chromosome 4 at 149,982,051 bp
  • T to C, chromosome 4 at 154,881,677 bp
  • T to C, chromosome 5 at 9,130,654 bp
  • T to G, chromosome 5 at 81,648,713 bp
  • T to A, chromosome 5 at 128,601,582 bp
  • A to T, chromosome 6 at 129,428,864 bp
  • A to T, chromosome 7 at 12,991,208 bp
  • T to A, chromosome 7 at 23,540,898 bp
  • A to G, chromosome 7 at 25,686,017 bp
  • A to G, chromosome 7 at 45,735,188 bp
  • A to C, chromosome 7 at 85,858,238 bp
  • T to C, chromosome 7 at 100,160,371 bp
  • G to A, chromosome 7 at 105,901,980 bp
  • T to C, chromosome 8 at 37,526,289 bp
  • A to G, chromosome 8 at 108,951,392 bp
  • T to A, chromosome 9 at 7,102,549 bp
  • A to G, chromosome 9 at 38,464,247 bp
  • A to T, chromosome 9 at 57,258,736 bp
  • A to G, chromosome 9 at 74,210,646 bp
  • A to G, chromosome 9 at 108,024,435 bp
  • A to T, chromosome 10 at 59,435,945 bp
  • T to A, chromosome 10 at 77,901,032 bp
  • T to C, chromosome 11 at 3,973,330 bp
  • T to C, chromosome 11 at 105,389,777 bp
  • C to A, chromosome 11 at 118,041,484 bp
  • A to G, chromosome 11 at 121,222,281 bp
  • C to G, chromosome 12 at 30,015,505 bp
  • A to G, chromosome 12 at 84,156,459 bp
  • T to C, chromosome 15 at 8,303,485 bp
  • T to C, chromosome 15 at 74,840,949 bp
  • C to T, chromosome 16 at 58,717,781 bp
  • C to T, chromosome 16 at 76,292,588 bp
  • A to G, chromosome 16 at 97,568,437 bp
  • A to T, chromosome 19 at 5,083,091 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6921 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045006-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.