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Strain Name:
C57BL/6J-MtgxR6862Btlr/Mmmh
Stock Number:
045026-MU
Citation ID:
RRID:MMRRC_045026-MU
Other Names:
R6862 (G1)
Major Collection:

Strain Information

Tk1
Name: thymidine kinase 1
Synonyms: Tk-1, D530002A18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 21877
Homologene: 37749
Hdgfl2
Name: HDGF like 2
Synonyms: HRP-2, Hdgfrp2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15193
VEGA: 17
Homologene: 7355
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Phf3
Name: PHD finger protein 3
Synonyms: 2310061N19Rik, AU020177
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213109
HGNC: HGNC:8921
Homologene: 9040
Washc4
Name: WASH complex subunit 4
Synonyms: A230046K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319277
VEGA: 10
Homologene: 123926
Nacc1
Name: nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
Synonyms: Nac1, 4930511N13Rik, 2010001H03Rik, Btbd14b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66830
VEGA: 8
Homologene: 12042
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 30,813,982 bp
  • C to T, chromosome 1 at 34,439,283 bp
  • C to A, chromosome 1 at 38,406,497 bp
  • T to A, chromosome 1 at 44,206,366 bp
  • T to A, chromosome 1 at 71,613,907 bp
  • T to A, chromosome 1 at 140,102,362 bp
  • T to C, chromosome 1 at 171,601,155 bp
  • A to T, chromosome 2 at 36,850,222 bp
  • T to A, chromosome 3 at 79,832,099 bp
  • C to A, chromosome 3 at 97,767,024 bp
  • C to T, chromosome 3 at 138,186,188 bp
  • A to G, chromosome 4 at 52,855,695 bp
  • A to G, chromosome 4 at 73,687,384 bp
  • C to A, chromosome 4 at 83,012,014 bp
  • T to C, chromosome 5 at 8,989,641 bp
  • A to C, chromosome 5 at 25,310,517 bp
  • C to A, chromosome 5 at 30,147,979 bp
  • A to G, chromosome 5 at 32,827,759 bp
  • T to G, chromosome 5 at 34,288,105 bp
  • T to G, chromosome 5 at 129,903,185 bp
  • T to C, chromosome 6 at 39,111,736 bp
  • A to T, chromosome 6 at 50,227,398 bp
  • C to T, chromosome 7 at 18,521,398 bp
  • T to A, chromosome 7 at 49,770,918 bp
  • T to C, chromosome 7 at 56,773,311 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • T to A, chromosome 7 at 104,363,753 bp
  • T to C, chromosome 7 at 123,321,901 bp
  • A to G, chromosome 7 at 133,587,273 bp
  • C to T, chromosome 7 at 141,809,744 bp
  • A to G, chromosome 8 at 11,202,926 bp
  • C to A, chromosome 8 at 27,113,436 bp
  • T to A, chromosome 8 at 27,113,437 bp
  • G to A, chromosome 8 at 84,673,215 bp
  • G to A, chromosome 8 at 105,328,230 bp
  • T to C, chromosome 9 at 27,030,809 bp
  • T to A, chromosome 9 at 38,930,476 bp
  • T to C, chromosome 10 at 14,130,583 bp
  • G to T, chromosome 10 at 50,849,646 bp
  • T to A, chromosome 10 at 83,558,893 bp
  • T to A, chromosome 10 at 107,686,225 bp
  • A to T, chromosome 10 at 118,205,810 bp
  • G to A, chromosome 11 at 46,407,591 bp
  • C to A, chromosome 11 at 54,055,848 bp
  • G to T, chromosome 11 at 58,995,453 bp
  • A to T, chromosome 11 at 82,719,959 bp
  • G to A, chromosome 11 at 91,575,915 bp
  • A to T, chromosome 11 at 104,721,458 bp
  • A to G, chromosome 11 at 117,816,494 bp
  • A to T, chromosome 12 at 4,216,539 bp
  • G to T, chromosome 12 at 100,061,689 bp
  • T to C, chromosome 13 at 104,150,152 bp
  • T to C, chromosome 14 at 36,940,081 bp
  • A to G, chromosome 15 at 50,831,605 bp
  • T to C, chromosome 15 at 54,588,357 bp
  • A to T, chromosome 15 at 82,390,138 bp
  • A to T, chromosome 16 at 49,763,804 bp
  • C to A, chromosome 17 at 56,099,211 bp
  • T to A, chromosome 18 at 58,124,321 bp
  • T to C, chromosome 19 at 4,150,771 bp
  • C to T, chromosome 19 at 10,916,513 bp
  • T to A, chromosome 19 at 45,582,748 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6862 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045026-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.