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Strain Name:
C57BL/6J-MtgxR6962Btlr/Mmmh
Stock Number:
045072-MU
Citation ID:
RRID:MMRRC_045072-MU
Other Names:
R6962 (G1)
Major Collection:

Strain Information

Gsc2
Name: goosecoid homebox 2
Synonyms: 4930568H22Rik, Gscl
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 195333
HGNC: HGNC:4613
Homologene: 3884
Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Unc93b1
Name: unc-93 homolog B1, TLR signaling regulator
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54445
Homologene: 41325
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Fgd4
Name: FYVE, RhoGEF and PH domain containing 4
Synonyms: Frabin-gamma, Frabin-beta, Frabin-alpha, Frabin, 9330209B17Rik, ZFYVE6
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224014
Homologene: 26727
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
P4htm
Name: prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Synonyms: 4933406E20Rik, P4h-tm
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74443
Homologene: 41765
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 21,505,793 bp
  • A to G, chromosome 1 at 74,855,816 bp
  • T to A, chromosome 1 at 74,882,140 bp
  • T to G, chromosome 1 at 91,357,415 bp
  • A to G, chromosome 1 at 100,274,472 bp
  • G to A, chromosome 2 at 13,348,029 bp
  • A to T, chromosome 2 at 37,436,094 bp
  • A to G, chromosome 2 at 62,372,830 bp
  • A to G, chromosome 2 at 87,753,727 bp
  • G to A, chromosome 3 at 68,994,545 bp
  • A to C, chromosome 3 at 79,489,303 bp
  • A to G, chromosome 3 at 89,238,996 bp
  • A to T, chromosome 3 at 93,773,927 bp
  • A to G, chromosome 3 at 100,951,137 bp
  • T to C, chromosome 4 at 42,793,323 bp
  • G to T, chromosome 4 at 62,700,715 bp
  • A to G, chromosome 4 at 63,319,501 bp
  • T to G, chromosome 4 at 119,422,178 bp
  • C to T, chromosome 4 at 123,440,722 bp
  • G to A, chromosome 5 at 16,615,754 bp
  • G to T, chromosome 5 at 34,899,771 bp
  • T to A, chromosome 5 at 97,031,238 bp
  • A to G, chromosome 5 at 135,962,921 bp
  • A to G, chromosome 5 at 137,112,647 bp
  • C to T, chromosome 5 at 149,048,823 bp
  • C to T, chromosome 6 at 29,482,840 bp
  • A to T, chromosome 6 at 57,515,660 bp
  • G to T, chromosome 7 at 4,919,550 bp
  • A to T, chromosome 7 at 6,481,009 bp
  • C to T, chromosome 7 at 18,529,754 bp
  • A to T, chromosome 7 at 44,657,939 bp
  • T to G, chromosome 7 at 79,665,897 bp
  • T to G, chromosome 7 at 82,869,265 bp
  • T to C, chromosome 7 at 103,418,911 bp
  • T to A, chromosome 7 at 104,975,373 bp
  • C to T, chromosome 7 at 123,296,432 bp
  • C to A, chromosome 7 at 126,866,916 bp
  • A to T, chromosome 7 at 127,384,315 bp
  • A to G, chromosome 7 at 141,217,590 bp
  • A to G, chromosome 7 at 143,781,062 bp
  • G to A, chromosome 8 at 15,117,741 bp
  • A to G, chromosome 8 at 23,902,165 bp
  • C to A, chromosome 8 at 105,268,706 bp
  • T to C, chromosome 8 at 105,483,647 bp
  • T to C, chromosome 8 at 106,265,219 bp
  • A to G, chromosome 9 at 24,769,740 bp
  • T to C, chromosome 9 at 55,859,771 bp
  • T to C, chromosome 9 at 104,181,009 bp
  • C to A, chromosome 9 at 108,579,195 bp
  • A to G, chromosome 10 at 22,286,154 bp
  • A to G, chromosome 10 at 130,425,583 bp
  • T to A, chromosome 11 at 6,402,455 bp
  • T to C, chromosome 11 at 40,707,415 bp
  • C to A, chromosome 11 at 77,504,643 bp
  • A to G, chromosome 11 at 85,890,259 bp
  • G to A, chromosome 11 at 103,614,300 bp
  • G to T, chromosome 11 at 103,733,689 bp
  • T to A, chromosome 11 at 109,521,669 bp
  • A to T, chromosome 12 at 112,766,854 bp
  • A to T, chromosome 12 at 116,211,778 bp
  • G to T, chromosome 13 at 16,858,021 bp
  • G to T, chromosome 14 at 14,746,021 bp
  • G to C, chromosome 14 at 37,060,095 bp
  • A to G, chromosome 15 at 82,064,949 bp
  • T to A, chromosome 16 at 16,484,087 bp
  • T to C, chromosome 16 at 17,915,038 bp
  • T to A, chromosome 16 at 19,605,922 bp
  • T to C, chromosome 17 at 14,681,820 bp
  • T to G, chromosome 17 at 18,598,021 bp
  • C to G, chromosome 17 at 21,690,926 bp
  • T to C, chromosome 17 at 24,364,726 bp
  • T to A, chromosome 17 at 28,878,481 bp
  • T to C, chromosome 17 at 34,732,166 bp
  • G to T, chromosome 18 at 10,547,327 bp
  • G to A, chromosome 18 at 63,865,288 bp
  • T to G, chromosome 19 at 3,936,303 bp
  • G to T, chromosome 19 at 4,001,831 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6962 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045072-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.